Quantitative relationship between yeasts and bacteria in denture‐induced stomatitis

Budtz-Jørgensen, E; Theilade, Else; Theilade, J.

doi:10.1111/j.1600-0722.1983.tb00790.x

Cited by 58 publications

(66 citation statements)

References 20 publications

(16 reference statements)

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“…Symptoms of Candida-associated denture stomatitis range from mild to severe, including palatal edema, painful inflammation, and papillary hyperplasia (small pebble-like sores) (49). Denture stomatitis can have a negative impact on the quality of life for those affected, with very high recurrence rates despite treatment with antifungal therapy (2,3,5,7,12,38,47). Despite its prevalence, the pathogenesis of DS and the role of the immune response are not understood.…”

Section: Enture Stomatitis (Ds) Is An Inflammatory Fungal Infectionmentioning

confidence: 99%

Development of a Contemporary Animal Model of Candida albicans-Associated Denture Stomatitis Using a Novel Intraoral Denture System

et al. 2012

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bDenture stomatitis (DS) is a fungal infection characterized by inflammation of the oral mucosa in direct contact with the denture and affects up to 50% of denture wearers. Despite the prevalence, very little is known about the role of fungal or host factors that contribute to pathogenesis. Recently, we developed a novel intraoral denture system for rodent research. This denture system consists of custom-fitted fixed and removable parts to allow repeated sampling and longitudinal studies. The purpose of this study was to use this denture system to develop a clinically relevant animal model of DS. To establish DS, rats were inoculated with pelleted Candida albicans, which resulted in sustained colonization of the denture and palate for 8 weeks postinoculation. Biofilm formation on the denture was observed by week 4 and on the palate by week 6 postinoculation. Rats were monitored for clinical signs of disease by assigning a clinical score after macroscopic examination of the palate tissue according to Newton's method. By week 4 postinoculation, the majority of inoculated rats with dentures exhibited a clinical score of 1 (pinpoint erythema). By week 6 and week 8 postinoculation, increasing percentages of rats exhibited a clinical score of 2 (diffuse erythema/ edema). Histological analysis of palate tissue demonstrated progressively increasing inflammatory cell recruitment throughout the time course of the infection. Palatal biofilm formation was commensurate with development of palatal erythema, which suggests a role for biofilm in the inflammatory response.

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Section: Enture Stomatitis (Ds) Is An Inflammatory Fungal Infectionmentioning

confidence: 99%

Development of a Contemporary Animal Model of Candida albicans-Associated Denture Stomatitis Using a Novel Intraoral Denture System

et al. 2012

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“…Plaque scores on denture fi tting surfaces were signifi cantly higher than on polished surfaces from the blind image scores, in agreement with previous studies. 15,24 Greater plaque coverage on the fi tting surface was expected since this surface is more diffi cult to clean due to the uneven surface features allowing plaque retention. Indeed, the control of fi tting surface plaque is important for clinical reasons, while for tooth surfaces aesthetics have a role to play.…”

Section: Assessment Of Denture Cleanser Effi Cacy In a Clinical Studymentioning

confidence: 99%

Evaluation of in vivo denture plaque assessment methods

Coulthwaite

2009

Br Dent J

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epidemiological studies of periodontal disease. [8][9][10] For clinical trials with denture cleansing products however, a broader approach can be taken, in that scores per denture surface are suffi cient and appropriate to judge the effi cacy of products. Current methods tend to rely on subjective or image analysis derived estimates of area coverage of dentures by stained plaque. 5,7,[11][12][13] For denture plaque assessment most methods assess plaque coverage by staining with a disclosing agent, most commonly erythrosine. Budtz-Jörgensen and Bertram 11 proposed a simple index to grade plaque coverage on the denture fi tting surface: excellent -none or only a few spots of plaque; fair -<50% denture base covered; and poor ->50% denture base covered. A slightly more sensitive method was described in 1978 14 where the amount of denture plaque on the fi tting surface was graded 0 = non-visible, 1 + = less than one third covered, 2 + = one third to two thirds covered, and 3 + = more than two thirds covered. Augsberger and Elahi 12 developed a well-used index for INTRODUCTIONMeasurement and assessment of denture plaque can provide valuable information regarding oral health status associated with denture wearing and assessment of new treatments or products. The potential pathogenic aspects of denture plaque have been recently reviewed. 1 Methods for assessing plaque quantity have included dry or wet weight measurement, biochemical assays, oxygen consumption assays, microbiological counts and visual indices or planimetric assessments (area measurement) of plaque coverage or biofi lm thickness in situ.2-7 Many scoring systems used for dentures have derived from studies on natural teeth, in which individual tooth plaque measurements have been used in Background Measurement and assessment of denture plaque can provide valuable information regarding an individual's oral health status and assessment of new treatments or products. Current methods tend to rely on subjective indices or image analysis derived planimetric (area measurement) assessment of stained plaque on dentures. Plaque indices are most commonly used to assess plaque coverage without image capture. This is not ideal because the methods are subjective, examiner bias may occur, there is no reproducibility between studies, the methods have lower accuracy and sensitivity than image analysis, and there is no record. To the authors' knowledge, no standardised published method of denture plaque assessment is currently employed for product development and testing. Method In this study visual and planimetric plaque assessment methods were compared using reference dentures. In addition, an in vivo study compared these methods for evaluating denture cleanser effi cacy. Results and conclusions The results show that blinded image scoring is more representative of the true plaque area coverage than 'live' denture scoring, detecting signifi cant decreases in plaque coverage. Planimetric analysis provides a more sensitive and less subjective technique with greater differentia...

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“…In the human mouth, C. albicans colonizes mucosal and prosthetic surfaces while interacting with a wide range of oral Streptococcus species, which can lead to the formation of complex microbial communities (17,18). Pathological changes in the oral mucosa under prostheses, known as denture stomatitis, are primarily caused by trauma, a lack of access of saliva, and C. albicans colonization of both soft-tissue and acrylic surfaces (9). However, oral streptococci, particularly Streptococcus gordonii and Streptococcus sanguinis, are also frequently detected in addition to C. albicans (9,17,57).…”

mentioning

confidence: 99%

“…Pathological changes in the oral mucosa under prostheses, known as denture stomatitis, are primarily caused by trauma, a lack of access of saliva, and C. albicans colonization of both soft-tissue and acrylic surfaces (9). However, oral streptococci, particularly Streptococcus gordonii and Streptococcus sanguinis, are also frequently detected in addition to C. albicans (9,17,57). Although much of the prior research has focused on the role of the fungus in soft-tissue complications and its interactions with S. gordonii, C. albicans may also influence the pathogenesis of dental caries disease (24), particularly in early childhood caries (ECC) (12,37,39,50).…”

mentioning

confidence: 99%

Role of Glucosyltransferase B in Interactions of Candida albicans with Streptococcus mutans and with an Experimental Pellicle on Hydroxyapatite Surfaces

Gregoire

Xiao

Silva

et al. 2011

Appl Environ Microbiol

166

218

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Candida albicans and mutans streptococci are frequently detected in dental plaque biofilms from toddlers afflicted with early childhood caries. Glucosyltransferases (Gtfs) secreted by Streptococcus mutans bind to saliva-coated apatite (sHA) and to bacterial surfaces, synthesizing exopolymers in situ, which promote cell clustering and adherence to tooth enamel. We investigated the potential role Gtfs may play in mediating the interactions between C. albicans SC5314 and S. mutans UA159, both with each other and with the sHA surface. GtfB adhered effectively to the C. albicans yeast cell surface in an enzymatically active form, as determined by scintillation spectroscopy and fluorescence imaging. The glucans formed on the yeast cell surface were more susceptible to dextranase than those synthesized in solution or on sHA and bacterial cell surfaces (P < 0.05), indicating an elevated ␣-1,6-linked glucose content. Fluorescence imaging revealed that larger numbers of S. mutans cells bound to C. albicans cells with glucans present on their surface than to yeast cells without surface glucans (uncoated). The glucans formed in situ also enhanced C. albicans interactions with sHA, as determined by a novel single-cell micromechanical method. Furthermore, the presence of glucan-coated yeast cells significantly increased the accumulation of S. mutans on the sHA surface (versus S. mutans incubated alone or mixed with uncoated C. albicans; P < 0.05). These data reveal a novel cross-kingdom interaction that is mediated by bacterial GtfB, which readily attaches to the yeast cell surface. Surface-bound GtfB promotes the formation of a glucan-rich matrix in situ and may enhance the accumulation of S. mutans on the tooth enamel surface, thereby modulating the development of virulent biofilms.

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Quantitative relationship between yeasts and bacteria in denture‐induced stomatitis

Cited by 58 publications

References 20 publications

Development of a Contemporary Animal Model of Candida albicans-Associated Denture Stomatitis Using a Novel Intraoral Denture System

Development of a Contemporary Animal Model of Candida albicans-Associated Denture Stomatitis Using a Novel Intraoral Denture System

Evaluation of in vivo denture plaque assessment methods

Role of Glucosyltransferase B in Interactions of Candida albicans with Streptococcus mutans and with an Experimental Pellicle on Hydroxyapatite Surfaces

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