Quantitative real-time PCR detection and analysis of a lumpy skin disease outbreak in Inner Mongolia Autonomous Region, China

Li, Lin; Qi, Chuanxiang; Li, Jinming; Nan, Wenlong; Wang, Ying; Xing, Chao; Chi, Tianying; Gong, Maolian; Ha, Da; De, Jide; Wu, Xiaodong

doi:10.3389/fvets.2022.936581

Cited by 9 publications

(6 citation statements)

References 19 publications

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“…The con rmed cases of LSDV revealed 14.28% of positivity in blood, 95.04% of positivity in skin scab and 66.66% of positivity in nasal swab by speci c conventional PCR. Similarly the higher percentage of scab samples were found positive for LSDV in this study are in agreement with result of recent reports [40,41,42,43,44,27]. Moreover, skin nodule/scab samples are the most preferred sample types for LSD diagnosis and LSDV genome can be detected in skin lesions up to 92 days after infection [40].…”

Section: Discussionsupporting

confidence: 92%

Molecular characterization, phylogenetic analysis and viral load quantification of Lumpy Skin Disease Virus in Cattle

Nagraja,

Sajjanar

et al. 2023

Preprint

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Lumpy skin disease is an emerging, remerging and trans-boundary disease caused by lumpy skin disease virus (LSDV) belongs Capripoxvirus genus and Poxviridae family in cattle. The recent episodes of disease outbreak in last 4 years from 2019 to 2023 caused huge socio-economic loss. In the present study, clinical samples from LSD outbreaks were collected from different districts of Karnataka, India for virus isolation and molecular characterization. Preliminary confirmation of LSD virus was performed by targeting envelope protein p32 (192 bp) gene by conventional Polymerase chain reaction (PCR). Real-Time qPCR was developed for absolute quantification of viral copy numbers in the infected samples. The viral copy number Log10 (Mean ± SD) in majority of the representative samples ranged from 4.962 to 12.432. The partial genes of GPCR and P32 were sequenced and submitted to GenBank. Phylogenetic analysis was performed by comparing sequences with other LSDV, Sheep pox virus (SPPV) and Goat pox virus (GPPV). The results indicated that our isolates have similarity with the LSDV sequences from Nepal, Bangladesh, Botswana, Egypt, Kenya Turkey and Saudi Arabia. It was also found that one of our isolate (OQ383668) clustered with the GPPV with 100% similarity. Our results highlight the necessity of ongoing surveillance and molecular characterization of circulating LSDV strains required for perspective development of suitable LSD vaccine to control the disease.

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Section: Discussionsupporting

confidence: 92%

Molecular characterization, phylogenetic analysis and viral load quantification of Lumpy Skin Disease Virus in Cattle

Nagraja,

Sajjanar

et al. 2023

Preprint

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“…In outbreaks these viruses display variability in morbidity, mortality and severity of clinical disease. The patterns of virus replication and secretion between experimental infection of classical LSDV (Babiuk et al, 2008b) and recombinant LSDV observed in the field are similar (Li et al, 2022).…”

Section: Similarities and Differences Between Classical And Recombina...mentioning

confidence: 69%

Lumpy skin disease: history, current understanding and research gaps in the context of recent geographic expansion

Mazloum,

Van Schalkwyk,

Babiuk

et al. 2023

Front. Microbiol.

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Lumpy skin disease is recognized as a transboundary and emerging disease of cattle, buffaloes and other wild ruminants. Being initially restricted to Africa, and since 1989 the Middle East, the unprecedented recent spread across Eurasia demonstrates how underestimated and neglected this disease is. The initial identification of the causative agent of LSD as a poxvirus called LSD virus, was well as findings on LSDV transmission and epidemiology were pioneered at Onderstepoort, South Africa, from as early as the 1940s by researchers such as Weiss, Haig and Alexander. As more data emerges from an ever-increasing number of epidemiological studies, previously emphasized research gaps are being revisited and discussed. The currently available knowledge is in agreement with the previously described South African research experience that LSDV transmission can occur by multiple routes, including indirect contact, shared water sources and arthropods. The virus population is prone to molecular evolution, generating novel phylogenetically distinct variants resulting from a diverse range of selective pressures, including recombination between field and homologous vaccine strains in cell culture that produce virulent recombinants which pose diagnostic challenges. Host restriction is not limited to livestock, with certain wild ruminants being susceptible, with unknown consequences for the epidemiology of the disease.

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“…In recent years, Taqman-Based qPCR methods have been developed, and widely applied for detecting LSDV [20][21][22], since Taqman-Based qPCR method showed higher sensitivity than the SYBR Green qPCR methods and PCR methods [23]. In this study, a Taqman-Based qPCR approach was successfully established for LSDV detection, the ampli cation e ciency (E) and correlation coe cient (R 2 ) of the standard curve linearity was 0.9968 and 98.48%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology and genetic characteristics of the lumpy skin disease virus in Yunnan province, China from 2019 to 2023

Yao,

Lin,

Zhu

et al. 2024

Preprint

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Background Lumpy skin disease (LSD) is caused by the lumpy skin disease virus (LSDV) and has emerged as a significant threat to the cattle and water buffalo industries in China. Investigating the epidemiological characteristics of LSDV will be beneficial to the control and prevention of this disease. In this study, a Taqman-Probe-Based real-time PCR (qPCR) assay was developed for detecting LSDV nucleotides, 2495 tissue or blood samples were collected from Yunnan province and tested using the established qPCR assay. The GPCR and p32 genes of the LSDV-positive samples were amplified, sequenced, and analyzed. Additionally, an infectious LSDV strain was isolated from one positive sample, and its biological characteristics were explored. Results The established qPCR assay demonstrated high sensitivity with a limit of detection of 4.83 copies/µL. Out of the 2495 collected specimens, six (0.24%) samples were positive for LSDV nucleotides, and all positive samples belonged to the skin scab samples. Molecular and phylogenetic analysis based on the P32 and GPCR genes revealed that six LSDV strains were genetically similar to field LSDV strains from China, Russia, and Thailand. One LSDV strain was isolated and replicated well in PST cells, while which was not sensitive to the MDBK cells. Conclusions This study provides the first report on the epidemiological characteristics of LSDV in Yunnan province of China, which confirmed the prevalence of LSDV in Cattle in this province. These findings will contribute to the development of effective strategies for the prevention and control of LSDV.

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Quantitative real-time PCR detection and analysis of a lumpy skin disease outbreak in Inner Mongolia Autonomous Region, China

Cited by 9 publications

References 19 publications

Molecular characterization, phylogenetic analysis and viral load quantification of Lumpy Skin Disease Virus in Cattle

Molecular characterization, phylogenetic analysis and viral load quantification of Lumpy Skin Disease Virus in Cattle

Lumpy skin disease: history, current understanding and research gaps in the context of recent geographic expansion

Epidemiology and genetic characteristics of the lumpy skin disease virus in Yunnan province, China from 2019 to 2023

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