Quantitative rapid steady state T₁ magnetic resonance imaging for cerebral blood volume mapping in mice: Lengthened measurement time window with intraperitoneal Gd‐DOTA injection

Abstract

This work demonstrates how the rapid steady state T1 MRI technique for cerebral blood volume fraction (BVf) quantification can be used with intraperitoneal Gd-DOTA injections in mice at 4.7 T. The peak signal amplitude after intravenous administration (0.7 mmol/kg) and the steady state signal amplitude reached 15 min after intraperitoneal administration (6 mmol/kg) in the same mice lead to equivalent BVf measures in the order of 0.02 in the brain. The resulting time window for BVf quantification is ≈30 min and allows for cerebral BVf mapping with increased spatial resolution or signal-to-noise ratio, or for monitoring functional BVf changes. A cerebral BVf increase of up to 25% induced by the vasodilator acetazolamide was observed, validating the vascular origin of the signal. The noninvasive and quantitative rapid steady state T1 technique can be used in serial studies to evaluate new drugs or disease models, such as antiangiogenic therapies in tumors.

Rapid-Steady-State-T1signal modeling during contrast agent extravasation: Toward tumor blood volume quantification without requiring the arterial input function

“…The ability to derive BVf and the degree of BBB permeability can aid in improving diagnostic sensitivity and specificity in neuro-oncology imaging and in monitoring the tumor tissue response to antiangiogenic therapies. A good sensitivity of the RSST 1 method to cerebral BVf changes has already been shown under hypercapnia (10) and the vasodilator acetazolamide (11). This work also demonstrates that the tumor BVf can be quantified with a clinically approved low-molecular size CA simplifying the clinical transfer of the method.…”

3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats

“…Consequently, most of these studies are performed ex-vivo . On the contrary, perfusion MRI is a clinical routine technique and is often applied for small animal imaging using 2D T1-weighed MR sequences 19, 20 . In our case, 3D high spatial resolution was necessary in order to discriminate differences of perfusion within the defects.…”

Microscopic DTI accurately identifies early glioma cell migration: correlation with multimodal imaging in a new glioma stem cell model

“…The RSST 1 method consists of a 3D inversion recovery sequence ( T R / T inv = 750/303 ms) and a series of RF pulses ( α = 10°, T R / T E = 6.5/1.2 ms,). The RSST 1 signal was acquired for 5 min before ( S pre ) and for 55 min after ( S post ) intraperitoneal Gd‐DOTA injection (6 mmol/kg, 12 ml/kg) with a temporal resolution of 6 s (matrix 8 × 32 × 32).…”

“…The RSST 1 signal was acquired for 5 min before ( S pre ) and for 55 min after ( S post ) intraperitoneal Gd‐DOTA injection (6 mmol/kg, 12 ml/kg) with a temporal resolution of 6 s (matrix 8 × 32 × 32). In this case, blood volume fraction (BVf) quantification is derived from the second period after injection (20–35 min), since the steady state signal is established 15 min later compared with intravenous injection . The last 25 min was here used especially to follow the signal increase in the case of permeable tumor vessels.…”