Quantitative radioligand assays using de novo–synthesized recombinant autoantigens in connective tissue diseases: New tools to approach the pathogenic significance of anti-RNP antibodies in rheumatic diseases

Yamamoto, Ana Maria; Amoura, Zahir; Johannet, C.; Jerônimo, Antônio Luiz C.; Campos, Henry de Holanda; Koutouzov, Sophie; Piette, Jean‐Charles; Bach, Jean‐François

doi:10.1002/1529-0131(200003)43:3<689::aid-anr27>3.0.co;2-u

Cited by 41 publications

(31 citation statements)

References 46 publications

(23 reference statements)

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“…Radioligand assay detects natural conformational epitope of antigens, while the immunoblotting method may detect denatured antigen. It has already been shown that radioligand assay detects autoantibodies even against conformational antigens [17,18]. O'Dwyer et al [6] purified enolase using goat polyclonal antienolase antibodies (C-19) (Santa Cruz Biotechnology, Inc., Santa Cruz, CA), but in our study these antibodies could not detect recombinant alpha-enolase by radioligand assay (data not shown).…”

Section: Discussioncontrasting

confidence: 59%

Anti-Alpha-Enolase Antibodies in Pituitary Disease

et al. 2003

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Abstract.A previous study reported a high prevalence of autoantibodies to alpha-enolase in lymphocytic hypophysitis and these antibodies efficiently distinguished lymphocytic hypophysitis from pituitary tumors. To confirm this, we examined autoantibodies to alpha-enolase in patients with lymphocytic hypophysitis (n = 17), pituitary non-functioning adenoma (n = 13), other pituitary diseases (n = 17) and other autoimmune diseases (n = 30), and compared to healthy controls (n = 46). Autoantibodies were found in 41.2%, 46.2%, 23.5%, 20.0% and 4.3%, respectively. Our findings indicate that detection of anti-alpha-enolase antibodies is not suitable for specific diagnosis of lymphocytic hypophysitis.

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Section: Discussioncontrasting

confidence: 59%

Anti-Alpha-Enolase Antibodies in Pituitary Disease

et al. 2003

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“…Because 9% of mothers who are seronegative at the time of fetal diagnosis later become seropositive, 10 and once they are detected, the antibodies permanently remain in the maternal serum, the mothers of all patients included in the present study systematically underwent, on inclusion, a screening for antibodies to soluble nuclear antigens 48-kd SSB/La, 52-kd SSA/Ro, and 60-kd SSA/Ro by use of previously described high-sensitivity, quantitative radioligand assays. 11 AV block was classified as congenital if it was diagnosed in utero, at birth, or during the first month of life and as childhood AV block if diagnosed between the first month and the fifteenth year of life. 12 The methodology and available data on the clinical characteristics of these patients have been reported previously.…”

Section: Clinical Investigationmentioning

confidence: 99%

Parental Electrocardiographic Screening Identifies a High Degree of Inheritance for Congenital and Childhood Nonimmune Isolated Atrioventricular Block

et al. 2012

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Background-The origin of congenital or childhood nonimmune isolated atrioventricular (AV) block remains unknown.We hypothesized that this conduction abnormality in the young may be a heritable disease. Methods and Results-A multicenter retrospective study (13 French referral centers, from 1980(13 French referral centers, from -2009 included 141 children with AV block diagnosed in utero, at birth, or before 15 years of age without structural heart abnormalities and without maternal antibodies. Parents and matched control subjects were investigated for family history and for ECG screening. In parents, a family history of sudden death or progressive cardiac conduction defect was found in 1.4% and 11.1%, respectively. Screening ECGs from 130 parents (mean age 42.0Ϯ6.8 years, 57 couples) were compared with those of 130 matched healthy control subjects. All parents were asymptomatic and in sinus rhythm, except for 1 with undetected complete AV block. Conduction abnormalities were more frequent in parents than in control subjects, found in 50.8% versus 4.6%, respectively (PϽ0.001). A long PR interval was found in 18.5% of the parents but never in control subjects (PϽ0.0001). Complete or incomplete right bundle-branch block was observed in 39.2% of the parents and 1.5% of the control subjects (PϽ0.0001). Complete or incomplete left bundle-branch block was found in 15.4% of the parents and 3.1% of the control subjects (PϽ0.0006). Estimated heritability for isolated conduction disturbances was 91% (95% confidence interval, 80%-100%). SCN5A mutation screening identified 2 mutations in 2 patients among 97 children. Conclusions-ECG screening in parents of children affected by idiopathic AV block revealed a high prevalence of conduction abnormalities. These results support the hypothesis of an inheritable trait in congenital and childhood nonimmune isolated AV block.

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“…Radioligand assay of the 75-kDa autoantigen reactivity was performed as described earlier 12 in the serum of each IPEX patient.…”

Section: Detection Of Anti-enterocyte Antibodiesmentioning

confidence: 99%

Digestive histopathological presentation of IPEX syndrome

et al. 2009

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Immunodysregulation, polyendocrinopathy, enteropathy, and X-linked inheritance (IPEX) syndrome is a well recognized and particularly severe form of autoimmune enteropathy. It has an X-linked recessive transmission, and is caused by mutations in the FOXP3 gene. We studied the intestinal morphological changes characterizing IPEX syndrome in a series of 12 children with a molecularly confirmed diagnosis. Histological examination of duodenal, gastric and colonic biopsies were retrospectively reviewed and compared by two independent experienced pathologists. In parallel, the presence of circulating anti-enterocyte antibodies was analysed using an indirect immunofluorescence technique and a quantitative radioligand assay against the 75-kDa autoantigen. The morphology of the inflammatory gut lesions could be categorized into three different entities, namely graft-vs-host disease-like changes (9/12 patients), a coeliac disease-like pattern (2/12) and an enteropathy with a complete depletion of goblet cells (1/12). Our results do not suggest any phenotypegenotype correlation. Circulating antibodies were detected in all 12 patients, with an anti-brush border pattern (11/12) and anti-goblet cell antibodies (1/12), as well as by a radioligand assay. The histological presentation of autoimmune enteropathy is rather variable. However, a graft-vs-host disease-like pattern associated with positive anti-enterocyte antibodies is the most frequent intestinal presentation of IPEX syndrome, and constitutes a very valuable tool for pathologists to suspect this diagnosis.

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Quantitative radioligand assays using de novo–synthesized recombinant autoantigens in connective tissue diseases: New tools to approach the pathogenic significance of anti-RNP antibodies in rheumatic diseases

Abstract: These highly sensitive, specific, and quantitative RLAs represent new tools for the detection of autoantibodies to RNP antigens in rheumatic diseases, and may be useful for (differential) diagnosis in clinical practice.

Cited by 41 publications

References 46 publications

Anti-Alpha-Enolase Antibodies in Pituitary Disease

Anti-Alpha-Enolase Antibodies in Pituitary Disease

Parental Electrocardiographic Screening Identifies a High Degree of Inheritance for Congenital and Childhood Nonimmune Isolated Atrioventricular Block

Digestive histopathological presentation of IPEX syndrome

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