Quantitative Proteomics Reveals that Plasma Membrane Microdomains From Poplar Cell Suspension Cultures Are Enriched in Markers of Signal Transduction, Molecular Transport, and Callose Biosynthesis

Srivastava, Vaibhav; Malm, Erik; Sundqvist, Gustav; Bulone, Vincent

doi:10.1074/mcp.m113.029033

Cited by 45 publications

(50 citation statements)

References 81 publications

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“…Error bars indicate SD. represent 15% of total proteins, PMA2 showed enrichment in the DIM fraction by a factor of 3.7. These results are consistent with previous studies mostly showing a preferential association of PM-associated H + -ATPases with DIM fractions isolated from plant tissues (Mongrand et al, 2004;Borner et al, 2005;Morel et al, 2006;Raffaele et al, 2009;Srivastava et al, 2013). From these data, we concluded that PDCB1 and PDLP1 may reside in different membrane lipid environments and that the GPIanchored protein PDCB1 is likely to reside in locally confined membrane environments enriched in sterols and sphingolipids.…”

Section: The Localization Of Pdcb1 and Pdbg2 Gpi-anchored Proteins Tosupporting

confidence: 82%

Specific Membrane Lipid Composition Is Important for Plasmodesmata Function in Arabidopsis

et al. 2015

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Plasmodesmata (PD) are nano-sized membrane-lined channels controlling intercellular communication in plants. Although progress has been made in identifying PD proteins, the role played by major membrane constituents, such as the lipids, in defining specialized membrane domains in PD remains unknown. Through a rigorous isolation of "native" PD membrane fractions and comparative mass spectrometry-based analysis, we demonstrate that lipids are laterally segregated along the plasma membrane (PM) at the PD cell-to-cell junction in Arabidopsis thaliana. Remarkably, our results show that PD membranes display enrichment in sterols and sphingolipids with very long chain saturated fatty acids when compared with the bulk of the PM. Intriguingly, this lipid profile is reminiscent of detergent-insoluble membrane microdomains, although our approach is valuably detergent-free. Modulation of the overall sterol composition of young dividing cells reversibly impaired the PD localization of the glycosylphosphatidylinositolanchored proteins Plasmodesmata Callose Binding 1 and the b-1,3-glucanase PdBG2 and altered callose-mediated PD permeability. Altogether, this study not only provides a comprehensive analysis of the lipid constituents of PD but also identifies a role for sterols in modulating cell-to-cell connectivity, possibly by establishing and maintaining the positional specificity of callosemodifying glycosylphosphatidylinositol proteins at PD. Our work emphasizes the importance of lipids in defining PD membranes.

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Section: The Localization Of Pdcb1 and Pdbg2 Gpi-anchored Proteins Tosupporting

confidence: 82%

Specific Membrane Lipid Composition Is Important for Plasmodesmata Function in Arabidopsis

et al. 2015

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“…Proteomic techniques bridge the gap between gene expression and physiological changes and provide a crucial tool to shed more light on the molecular mechanisms underlying plants’ adaptation to nutrient limitation . As an alternative of traditional 2DE, iTRAQ combined with MS/MS provides an easier access to low‐abundance proteins, as well as to membrane proteins .…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies

et al. 2016

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Male and female poplars (Populus cathayana Rehd.) respond differently to nitrogen (N) and phosphorus (P) deficiencies. In this study, an iTRAQ-based quantitative proteomic analysis was performed. N and P deficiencies caused 189 and 144 proteins to change in abundance in males and 244 and 464 in females, respectively. Compared to N- and P-deficient males, both N- and P-deficient females showed a wider range of changes in proteins that are involved in amino acid, carbohydrate and protein metabolism, and the sexual differences were significant. When comparing the effects of N- and P-deficiencies, N-deficient females expressed more changes in proteins that are involved in stress responses and gene expression regulation, while P-deficient females showed more changes in proteins that are involved in energy and lipid metabolism, stress responses and gene expression regulation. The quantitative RT-PCR analysis of stress-related proteins showed that males have a better expression correlation between mRNA and protein levels than do females. This study shows that P. cathayana females are more sensitive and have more rapid metabolic mechanisms when responding to N and P deficiencies than do males, and P deficiency has a wider range of effects on females than does N deficiency.

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“…The function of the remaining four GSLs has not been clarified. Similar to the CesAs, GSLs are integrated into the plasma membrane, with a putative preference for detergent-resistant membrane regions [56], and are predicted to contain 14 to 16 transmembrane domains [57].…”

Section: Callose Synthesismentioning

confidence: 99%

Cellulose and callose synthesis and organization in focus, what's new?

Schneider

Hanak

Persson

et al. 2016

Current Opinion in Plant Biology

102

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Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/) eprints@whiterose.ac.uk https://eprints.whiterose.ac.uk/ Reuse Unless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. Abstract Plant growth and development are supported by plastic but strong cell walls. These walls consist largely of polysaccharides that vary in content and structure. Most of the polysaccharides are produced in the Golgi apparatus and are then secreted to the apoplast and built into the growing walls. However, the two glucan polymers cellulose and callose are synthesized at the plasma membrane by cellulose or callose synthase complexes, respectively. Cellulose is the most common cell wall polymer in land plants and provides strength to the walls to support directed cell expansion. In contrast, callose is integral to specialized cell walls, such as the cell plate that separates dividing cells and growing pollen tube walls, and maintains important functions during abiotic and biotic stress responses. The last years have seen a dramatic increase in our understanding of how cellulose and callose are manufactured, and new factors that regulate the synthases have been identified. Much of this knowledge has been amassed via various microscopy-based techniques, including various confocal techniques and super-resolution imaging. Here, we summarize and synthesize recent findings in the fields of cellulose and callose synthesis in plant biology. Title

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Quantitative Proteomics Reveals that Plasma Membrane Microdomains From Poplar Cell Suspension Cultures Are Enriched in Markers of Signal Transduction, Molecular Transport, and Callose Biosynthesis

Abstract: The plasma membrane (PM) is a highly dynamic interface that contains detergent-resistant microdomains (DRMs

Cited by 45 publications

References 81 publications

Specific Membrane Lipid Composition Is Important for Plasmodesmata Function in Arabidopsis

Specific Membrane Lipid Composition Is Important for Plasmodesmata Function in Arabidopsis

iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies

Cellulose and callose synthesis and organization in focus, what's new?

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