Quantitative proteomics reveal the alterations in the spinal�cord after myocardial ischemia‑reperfusion injury in rats

Li, Shun‐Yuan; Li, Zhixiao; He, Zhigang; Wang, Qian; Li, Yu‐Juan; Yang, Qing; Wu, Duozhi; Zeng, Hao‐Long; Xiang, Hong‐Bing

doi:10.3892/ijmm.2019.4341

Cited by 9 publications

(10 citation statements)

References 68 publications

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“…AKT3 is involved in cardiomyocyte cell death ( 71 ). In rats, the expression of AKT3 is decreased in the spinal cord following myocardial ischemia-reperfusion injury ( 72 ). In addition, AKT3 deficiency is related to an increase in atherosclerosis ( 73 ).…”

Section: Discussionmentioning

confidence: 99%

Uncovering potential differentially expressed miRNAs and targeted mRNAs in myocardial infarction based on integrating analysis

Wang¹,

Cao²

2020

Mol Med Rep

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Myocardial infarction (Mi) is one of the leading causes of death globally. The aim of the present study was to find valuable micrornas (mirnas/mirs) and target mrnas in order to contribute to our understanding of the pathology of Mi. mirna and mrna data were downloaded for differential expression analysis. Then, a regulatory network between mirnas and mrnas was established, followed by function annotation of target mrnas. Thirdly, prognosis and diagnostic analysis of differentially methylated target mrnas were performed. Finally, an in vitro experiment was used to validate the expression of selected mirnas and target mrnas. a total of 19 differentially expressed mirnas and 1,007 differentially expressed mRNAs were identified. Several regulatory interaction pairs between mirna and mrnas were identified, such as hsa-mir-142-2p-long-chain-fattyacid-coa ligase 1 (acSl1), hsa-mir-15a-3p-nicotinamide phosphoribosyltransferase (naMPT), hsa-mir-33b-5p-regulator of G-protein signaling 2 (rGS2), hsa-mir-17-3p-Jun dimerization protein 2 (JdP2), hsa-mir-24-1-5p-aquaporin-9 (aQP9) and hsa-mir-34a-5p-STaT1/aKT3. of note, it was demonstrated that acSl1, naMPT, rGS2, JdP2, aQP9, STaT1 and aKT3 had diagnostic and prognostic values for patients with Mi. in addition, STaT1 was involved in the 'chemokine signaling pathway' and 'Jak-STaT signaling pathway'. aKT3 was involved in both the 'MaPK signaling pathway' and 'T cell receptor signaling pathway'. reverse transcription-quantitative Pcr validation of hsa-mir-142-3p, hsa-mir-15a-3p, hsa-mir-33b-5p, acSl1, naMPT, rGS2 and JdP2 expression was consistent with the bioinformatics analysis. In conclusion, the identified miRNAs and mRNAs may be involved in the pathology of Mi.

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Section: Discussionmentioning

confidence: 99%

Uncovering potential differentially expressed miRNAs and targeted mRNAs in myocardial infarction based on integrating analysis

Wang¹,

Cao²

2020

Mol Med Rep

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“…No curative treatment yet exists for SCI, and rehabilitation is the only therapeutic option that can contribute to functional improvement in patients. Mass spectrometry proteomics has become a powerful tool in biomedical research over the past 20 years [ 37 ], including its applications for studying the differential proteome in SCI [ 38 , 39 ]. In this context, here, we utilized an unbiased proteomic approach with two independent but complementary techniques (iTRAQ and 2D-DIGE) to search for protein biomarkers as indicators of improvement associated with the administration of GH in patients with SCI.…”

Section: Discussionmentioning

confidence: 99%

Effects of Growth Hormone Treatment and Rehabilitation in Incomplete Chronic Traumatic Spinal Cord Injury: Insight from Proteome Analysis

Martín-Rojas

Sastre-Oliva

Esclarín-Ruz

et al. 2020

JPM

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Despite promising advances in the medical management of spinal cord injury (SCI), there is still no available effective therapy to repair the neurological damage in patients who experience this life-transforming condition. Recently, we performed a phase II/III placebo-controlled randomized trial of safety and efficacy of growth hormone (GH) treatment in incomplete chronic traumatic spinal cord injury. The main findings were that the combined treatment of GH plus rehabilitation treatment is feasible and safe, and that GH but not placebo slightly improves the SCI individual motor score. Moreover, we found that an intensive and long-lasting rehabilitation program per se increases the functional outcome of SCI individuals. To understand the possible mechanisms of the improvement due to GH treatment (motor score) and due to rehabilitation (functional outcome), we used a proteomic approach. Here, we used a multiple proteomic strategy to search for recovery biomarkers in blood plasma with the potential to predict response to somatropin treatment and to delayed intensive rehabilitation. Forty-six patients were recruited and followed for a minimum period of 1 year. Patients were classified into two groups based on their treatment: recombinant somatropin (0.4 mg) or placebo. Both groups received rehabilitation treatment. Our strategy allowed us to perform one of the deepest plasma proteomic analyses thus far, which revealed two proteomic signatures with predictive value: (i) response to recombinant somatropin treatment and (ii) response to rehabilitation. The proteins implicated in these signatures are related to homeostasis, inflammation, and coagulation functions. These findings open novel possibilities to assess and therapeutically manage patients with SCI, which could have a positive impact on their clinical response.

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“…Rats were randomly divided into MIRI (model, n=13) and sham (control, n=13) groups. MIRI experiments were performed according to previously published protocols (18,(26)(27)(28)(29). The rats were maintained in deep anesthetic state on a heating pad using sodium pentobarbital (50 mg/kg; intraperitoneal), which was indicated by disappearance of the foot withdrawal reflex.…”

Section: Methodsmentioning

confidence: 99%

Characterization of novel lncRNAs in upper thoracic spinal cords of rats with myocardial ischemia‑reperfusion injuries

Wang

et al. 2021

Exp Ther Med

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Myocardial ischemia-reperfusion injury (MIRI) is a significant problem in clinical cardiology, and refers to a more serious myocardial injury caused by blood recanalization after a period of myocardial ischemia, as compared with injury caused by vascular occlusion. The spinal cord, as the primary afferent and efferent center of cardiac sensory and sympathetic nerve fibres, has received increased attention in recent years with regards to the regulation of MIRIs. Previous studies have revealed that MIRI has a strong correlation with the abnormal expression of long non-coding (lnc)RNAs in the myocardium; however, there are limited reports on the effects of the altered expression of lncRNAs in the spinal cord following MIRI. To investigate the expression patterns of lncRNAs in the spinal cord after MIRI and their potential role in the early stage of reperfusion, a MIRI model was established in rats. After 30 min of myocardial ischemia and 2 h of reperfusion, the upper thoracic spinal cord tissues were immediately dissected and isolated. lncRNAs and mRNAs in spinal cord tissues were screened using transcriptome sequencing technology, and the expression of several highly deregulated mRNAs, including Frs3, Zfp52, Dnajc6, Nedd4l, Tep1, Myef2, Tgfbr1, Fgf12, Mef2c, Tfdp1 and lncRNA, including ENSRNOT00000080713, ENSRNOT00000090564, ENSRNOT00000082588, ENSRNOT00000091080, ENSRNOT00000091570, ENSRNOT00000087777, ENSRNOT00000082061, ENSRNOT00000091108, ENSRNOT00000087028, ENSRNOT00000086475, were further validated via reverse transcription-quantitative PCR. The number of altered expressed lncRNAs was 126, among which there were 41 upregulated probe sets and 85 downregulated sets. A total of 470 mRNAs were differentially expressed, in which 231 probe sets were upregulated and 239 were downregulated. Gene Ontology analysis indicated that dysregulated transcripts related to biological processes were mainly associated with ‘cell-cell signaling’. Moreover, pathway analysis demonstrated significant changes in the ‘PI3K/Akt signaling pathway’ and the ‘p53 signaling pathway’. Thus, the altered expression of lncRNAs in the spinal cord may be of considerable importance in the process of MIRI. The present results could provide an insight into the potential roles and mechanism of lncRNAs during the early stage of reperfusion.

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Quantitative proteomics reveal the alterations in the spinal�cord after myocardial ischemia‑reperfusion injury in rats

Cited by 9 publications

References 68 publications

Uncovering potential differentially expressed miRNAs and targeted mRNAs in myocardial infarction based on integrating analysis

Uncovering potential differentially expressed miRNAs and targeted mRNAs in myocardial infarction based on integrating analysis

Effects of Growth Hormone Treatment and Rehabilitation in Incomplete Chronic Traumatic Spinal Cord Injury: Insight from Proteome Analysis

Characterization of novel lncRNAs in upper thoracic spinal cords of rats with myocardial ischemia‑reperfusion injuries

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