Characterization of novel lncRNAs in upper thoracic spinal cords of rats with myocardial ischemia‑reperfusion injuries

Abstract

Myocardial ischemia-reperfusion injury (MIRI) is a significant problem in clinical cardiology, and refers to a more serious myocardial injury caused by blood recanalization after a period of myocardial ischemia, as compared with injury caused by vascular occlusion. The spinal cord, as the primary afferent and efferent center of cardiac sensory and sympathetic nerve fibres, has received increased attention in recent years with regards to the regulation of MIRIs. Previous studies have revealed that MIRI has a strong correlation with the abnormal expression of long non-coding (lnc)RNAs in the myocardium; however, there are limited reports on the effects of the altered expression of lncRNAs in the spinal cord following MIRI. To investigate the expression patterns of lncRNAs in the spinal cord after MIRI and their potential role in the early stage of reperfusion, a MIRI model was established in rats. After 30 min of myocardial ischemia and 2 h of reperfusion, the upper thoracic spinal cord tissues were immediately dissected and isolated. lncRNAs and mRNAs in spinal cord tissues were screened using transcriptome sequencing technology, and the expression of several highly deregulated mRNAs, including Frs3, Zfp52, Dnajc6, Nedd4l, Tep1, Myef2, Tgfbr1, Fgf12, Mef2c, Tfdp1 and lncRNA, including ENSRNOT00000080713, ENSRNOT00000090564, ENSRNOT00000082588, ENSRNOT00000091080, ENSRNOT00000091570, ENSRNOT00000087777, ENSRNOT00000082061, ENSRNOT00000091108, ENSRNOT00000087028, ENSRNOT00000086475, were further validated via reverse transcription-quantitative PCR. The number of altered expressed lncRNAs was 126, among which there were 41 upregulated probe sets and 85 downregulated sets. A total of 470 mRNAs were differentially expressed, in which 231 probe sets were upregulated and 239 were downregulated. Gene Ontology analysis indicated that dysregulated transcripts related to biological processes were mainly associated with ‘cell-cell signaling’. Moreover, pathway analysis demonstrated significant changes in the ‘PI3K/Akt signaling pathway’ and the ‘p53 signaling pathway’. Thus, the altered expression of lncRNAs in the spinal cord may be of considerable importance in the process of MIRI. The present results could provide an insight into the potential roles and mechanism of lncRNAs during the early stage of reperfusion.

MYEF2: an immune infiltration-related prognostic factor in IDH-wild-type glioblastoma

“…It binds to the promoter of the myelin basic protein gene (MBP) and represses its transcription, leading to oligodendrocyte progenitor cell (OPC) differentiation disorder and demyelination disease [ 27 , 28 ]. MYEF2 has also been reported to be associated with haematopoietic stem cell generation [ 29 ] and myocardial ischemia–reperfusion injury [ 30 ]. However, little is known about the role of MYEF2 in tumor.…”

The Preventive Effect of Cardiac Sympathetic Denervation Induced by 6‐OHDA on Myocardial Ischemia–Reperfusion Injury: The Changes of lncRNA/circRNAs‐miRNA‐mRNA Network of the Upper Thoracic Spinal Cord in Rats

“…Recently, significant efforts have been made to understand the alterations of ncRNAs in different spinal cord segments and their contributions to specific outcomes of diseases [ 16 , 26 , 42 – 44 ]. The spinal cord is a complex and dynamic neural structure.…”

“…There is accumulating evidence of the interaction between the spinal cord and the heart [ 51 – 55 ]. We previously demonstrated the changes of novel lncRNAs in the upper thoracic spinal cord of rats with MIRI [ 42 ]. In recent years, there has been considerable effort to explore the relationship between cardiac sympathetic activity and cardiovascular diseases.…”

Effects of Resistance Training on Oxidative Stress Markers and Muscle Damage in Spinal Cord Injured Rats

“…Furthermore, concerning spinal cord injury and the surgical procedure to which the animals were submitted, some aspects should be considered. In this sense, the different segments of the spinal cord end up by interfering in specific disease outcomes [ 56 , 57 ]. The spinal cord is a complex and dynamic neural structure, whose neurons, when injured, tend to interfere with the generation of sympathetic activity in many autonomic targets, including the heart and blood vessels [ 58 , 59 ], with evidence of the interaction between the spinal cord.…”