Quantitative proteomic analysis of sub-MIC erythromycin inhibiting biofilm formation of S. suis in vitro

Zhao, Yue; Zhou, Yonggang; Huang, Qiong; Han, Qinghua; Liu, Bing; Cheng, Guangdong; Li, Yanhua

doi:10.1016/j.jprot.2014.12.019

Cited by 46 publications

(57 citation statements)

References 86 publications

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“…It can cause meningitis, arthritis, septicemia, bronchopneumonia, and other pathological conditions (Zhao et al, 2015). At present, at least 29 S. suis serotypes have been identified.…”

Section: Introductionmentioning

confidence: 99%

Sub-Minimum Inhibitory Concentrations of Rhubarb Water Extracts Inhibit Streptococcus suis Biofilm Formation

Ding

Lian

et al. 2017

Front. Pharmacol.

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Streptococcus suis is one of the most important swine pathogens, which can cause persistent infection by forming biofilms. In this study, sub-minimum inhibitory concentration (sub-MIC) of rhubarb water extracts were found to inhibit biofilm formation. Two-component signal transduction systems (TCSs), transcriptional regulators, and DNA binding proteins were compared under two conditions: (1) cells treated with sub-MIC rhubarb water extracts and (2) untreated cells. Using an isobaric tags for relative and absolute quantitation (iTRAQ) strategy, we found that TCSs constituent proteins of histidine kinase and response regulator were significantly down-regulated. This down-regulation can affect the transfer of information during biofilm formation. The transcriptional regulators and DNA binding proteins that can interact with TCSs and interrupt gene transcription were also significantly altered. For these reasons, the levels of protein expressions varied in different parts of the treated vs. untreated cells. In summary, rhubarb water extracts might serve as potential inhibitor for the control of S. suis biofilm formation. The change in TCSs, transcriptional regulators, and DNA binding proteins may be important factors in S. suis biofilm inhibition.

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“…It can cause meningitis, arthritis, septicemia, bronchopneumonia, and other pathological conditions (Zhao et al, 2015). At present, at least 29 S. suis serotypes have been identified.…”

Section: Introductionmentioning

confidence: 99%

Sub-Minimum Inhibitory Concentrations of Rhubarb Water Extracts Inhibit Streptococcus suis Biofilm Formation

Ding

Lian

et al. 2017

Front. Pharmacol.

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“…Due to the high antibiotic resistance of biofilms, novel drugs are required to treat biofilm infections. Previous studies have shown that the formation of biofilm can be inhibited with sub-minimum inhibitory concentrations of certain antibiotics, such as imipenem and erythromycin (36,37). However, the long-term use of antibiotics will give rise to drug-resistant bacteria.…”

Section: Discussionmentioning

confidence: 99%

Hyperoside inhibits biofilm formation of Pseudomonas aeruginosa

Sun

Feng

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Pseudomonas aeruginosa (P. aeruginosa) is a common pathogen in hospital-acquired infection and is readily able to form biofilms. Due to its high antibiotic resistance, traditional antibacterial treatments exert a limited effect on P. aeruginosa biofilm infections. It has been indicated that hyperoside inhibits P. aeruginosa PAO1 (PAO1) biofilm formation without affecting growth. Therefore, the current study examined the biofilm formation and quorum sensing (QS) system of PAO1 in the presence of hyperoside.Confocal laser scanning microscopy analysis demonstrated that hyperoside significantly inhibited biofilm formation. It was also observed that hyperoside inhibited twitching motility in addition to adhesion. Data from reverse transcription-quantitative polymerase chain reaction indicated that hyperoside inhibited the expression of lasR, lasI, rhlR and rhlI genes. These results suggest that the QS-inhibiting effect of hyperoside may lead to a reduction in biofilm formation. However, the precise mechanism of hyperoside on P. aeruginosa pathogenicity remains unclear and requires elucidation in additional studies. IntroductionPseudomonas aeruginosa (P. aeruginosa) is a common pathogen in hospital-acquired infections (1). Due to increasing multidrug resistance, P. aeruginosa infection is an increasingly common cause of mortality and morbidity (2). The mechanisms of antibiotic resistance in P. aeruginosa include the expression of multiple antibiotic modifying enzymes, antibiotic efflux pumps and acquisition of chromosomally or plasmid encoded antibiotic resistance genes. Additionally, chromosomal mutations and lower membrane permeability for the antibiotics also contribute to antibiotic resistance (3).P. aeruginosa is a biofilm-forming pathogen and is difficult to eradicate due to its high antibiotic resistance and the ability of the biofilm to evade the immune system (4-7). Quorum sensing (QS) is a system of stimuli and response correlated to population density. P. aeruginosa uses the QS system to coordinate gene expression according to the density of its local population. Thus, it can coordinate certain behaviors such as biofilm formation, virulence and antibiotic resistance. QS inhibitors (QSIs) are the most well reported alternative therapeutics that can be used to overcome the problem of increasing antibiotic resistance in P. aeruginosa. QSIs target the virulence of the organism and therefore are also termed antipathogenic drugs. The virulence of P. aeruginosa depends on its cell-to-cell communication system, or QS system that uses diffusible signaling molecules that accumulate with increasing cell density and allows P. aeruginosa to trigger coordinated responses and achieve outcomes that would otherwise remain impossible to achieve by individual bacterium (8). Previous studies have demonstrated that traditional treatments for bacteria exert some effect on biofilm infections (9,10). Therefore, the effects of constituents from marine organisms, traditional Chinese herbs and plants (11-13) on biofilm infe...

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“…Cultures were diluted to 1 × 10 6 colony-forming units (CFU)/mL using THB. Finally, 100 μL of cell suspensions were inoculated into the wells of a 96-well plates (Corning Costar® 3599 Corning, NY, USA) containing serial dilutions of tylosin culture medium as previously reported [32,33]. At the same time, negative control was set up as outlined in a previous study [33].…”

Section: Minimum Inhibitory Concentrations and Growth Ratesmentioning

confidence: 99%

“…Finally, 100 μL of cell suspensions were inoculated into the wells of a 96-well plates (Corning Costar® 3599 Corning, NY, USA) containing serial dilutions of tylosin culture medium as previously reported [32,33]. At the same time, negative control was set up as outlined in a previous study [33]. The inoculated microplates were incubated at 37°C for 24 h. The MICs of tylosin-resistant mutants to a variety of antibiotics, terramycin, chlortetracycline, ofloxacin, enrofloxacin, florfenicol and penicillin K (98% purity, Solaibao Biotechnology Co., Ltd, Beijing, China) were measured using the same procedure.…”

Section: Minimum Inhibitory Concentrations and Growth Ratesmentioning

confidence: 99%

“…The growth rates of S. suis ATCC 700794 treated with and without 1/4 MIC (0.125 µg/mL) tylosin were analyzed [33]. Briefly, S. suis ATCC 700794 treated without tylosin and with 1/4 MIC tylosin (0.125 µg/mL) were incubated at 37℃ for 12 h. Then, the samples were taken every hour and applied to the blood plate (THA with 5% (v/v) fetal bovine serum).…”

Section: Minimum Inhibitory Concentrations and Growth Ratesmentioning

confidence: 99%

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Analysis of multidrug resistance in Streptococcus suis ATCC 700794 under tylosin stress

et al. 2019

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Streptococcus suis is an important zoonotic pathogen. The massive use of tylosin and other antibiotics in swine production has led to the emergence of resistant phenotypes of S. suis. However, there are no adequate measures available to address the problem of bacterial resistance. This study involved the use of 1/4 MIC (0.125 µg/mL) of tylosin to investigate resistance-related proteins by S. suis ATCC 700794. Our results showed that 171 proteins were differentially expressed in S. suis tested with 1/4 MIC (0.125 µg/mL) of tylosin using iTRAQ-based quantitative proteomic methods. TCS, heat shock protein and elongation factors were differentially expressed at 1/4 MIC (0.125 µg/mL) of tylosin compared to non treated, control cells. Using quantitative RT-PCR analysis, we verified the relationship between the differentially expressed proteins in S. suis with different MIC values. The data showed that expression profile for elongation factor G (fusA), elongation factor Ts (tsf), elongation factor Tu (tuf), putative histidine kinase of the competence regulon, ComD (comD), putative competence-damage inducible protein (cinA) and protein GrpE (grpE), observed in tylosin-resistant S. suis, correlated with that of S. suis ATCC 700794 at 1/4 MIC (0.125 µg/mL). The MIC of tylosin-resistant showed high-level resistance in terramycin, chlortetracycline, ofloxacin and enrofloxacin. Our findings demonstrated the importance of elongation factors, TCS and heat shock protein during development of tylosin resistance in S. suis. Thus, our study will provide insight into new drug targets and help reduce bacterial multidrug resistance through development of corresponding inhibitors.

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Quantitative proteomic analysis of sub-MIC erythromycin inhibiting biofilm formation of S. suis in vitro

Cited by 46 publications

References 86 publications

Sub-Minimum Inhibitory Concentrations of Rhubarb Water Extracts Inhibit Streptococcus suis Biofilm Formation

Sub-Minimum Inhibitory Concentrations of Rhubarb Water Extracts Inhibit Streptococcus suis Biofilm Formation

Hyperoside inhibits biofilm formation of Pseudomonas aeruginosa

Analysis of multidrug resistance in Streptococcus suis ATCC 700794 under tylosin stress

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