Sub-Minimum Inhibitory Concentrations of Rhubarb Water Extracts Inhibit Streptococcus suis Biofilm Formation

Ding, Wenya; Li, Yanhua; Lian, He; Ai, Xiaoyu; Zhao, Yue; Yang, Yanbei; Han, Qinghua; Liu, Xin; Chen, Xueying; Zhang, He

doi:10.3389/fphar.2017.00425

Cited by 23 publications

(20 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous study from our laboratory observed that the water extract of R. o cinale can intervene with the bio lm formation of S.suis [37]. However, it is not clear which ingredients from R. o cinale played the major role in this process.…”

Section: Introductionmentioning

confidence: 87%

Intervene effect on Streptococcus suis biofilm formation of emodin extracted from Rheum officinale Baill

Tang¹,

Bai²,

Yang³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Streptococcus suis is a zoonotic pathogen that causes serious systemic infections in pigs and humans. It is a great threat to pig breeding and public health safety. The formation of biofilm was one of the main reasons that it’s difficult to cure. Rhubarb water extract can inhibit the formation of biofilm of Streptococcus suis, but the main functional ingredient was not clear. And what’s the potential mechanism of emodin intervene the biofilm formation? Importantly, the gene expression of luxS of Streptococcus suis was significantly decreased with emodin treatment, if there is a possibility that emodin could combine with LuxS protein which in turn inhibit the formation of biofilm? Methods Optimization of green ultrasonic extraction of emodin from R. officinale by Response Surface Methodology. The activity of antibacterial was evaluated by MIC assay. And the ability that intervene biofilm formation was evaluated through Crystal violet staining and SEM. The combination mode between emodin and LuxS protein was identified through molecular docking. Results The optimum extraction conditions (ethanol concentration of 80%, extraction time of 21 min, and liquid to solid ratio of 12.5:1 mL/g) were determined through the interaction analysis of different influencing factors and model verification experiment. The minimal inhibitory concentration (MIC) of RUEP against S.suis was 15.625 µg·mL-1, and sub-inhibitory concentrations of RUEP (1/2, 1/4, 1/8 and 1/16 MIC) significantly inhibited the biofilm formation of S. suis. Moreover, scanning electron microscopy analysis confirmed that RUEP can damage the layered colony community structure in biofilm and effectively intervene in the biofilm formation of Streptococcus suis. Through molecular docking analyzed, the inhibition of biofilm formation could be realized through the formation of hydrogen bond with residues His14 and Glu 60, π-π staking with His61、Phe80 and hydrophobic interaction with Cys127. Conclusion Through our study, we found emodin have good antibacterial and antibiofilm formation properties and the potential mechanism of emodin intervention on biofilm formation of Streptococcus suis was inferred.

show abstract

Section: Introductionmentioning

confidence: 87%

Intervene effect on Streptococcus suis biofilm formation of emodin extracted from Rheum officinale Baill

Tang¹,

Bai²,

Yang³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Specific methods of iTRAQ-based quantitative proteomic analyses were conducted using a method similar to that described in the literature [22]. In brief, the sample was lysed[23] followed by homogenisation; proteins in the sample were run on a sodium dodecyl sulphate polyacrylamide gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

Powerful solution for experimental cerebral malaria treatment: artesunate and tetramethylpyrazine

Jiang

Chen

Zheng

et al. 2019

Preprint

View full text Add to dashboard Cite

BackgroundCerebral malaria (CM) is a kind of serious neurological complication caused by the acute Plasmodium falciparum infection. About 300000 patients including children under 5 years old died from this disease every year. Even intravenous artesunate (Art) is employed as the most effictive drug in the treatment of CM, high incidence of death and neurological sequelae are still inevitable. Therefore, we assessed the combination of Art and tetramethylpyrazine (TMP), to treat experimental CM (ECM) in C57BL/6 mice infected with Plasmodium berghei ANKA (PbA). A non-biased whole brain quantitative proteomic analysis was also conducted to get some insight of the mechanism of the combinational treatment.ResultsTreatment of (ECM)-C57BL/6 mice with the combination of Art and TMP increased the survival, improved clinical signs and prevented neurological manifestations. These effects were related to reduction of parasitised red blood cells (pRBC) adhesion, sequestration, maintaining brain microvascular integrity, increasing nerve growth factor, neurotrophin levels, and alleviating hippocampal neuronal damage and astrocyte activation. The pharmacological effects of Art-TMP combination therapy were analyzed by ECM mice brain proteomic function enrichment. Based on an isobaric tag for relative and absolute quantitation (iTRAQ) fold-change of 1.2 (P-value < 0.05), 217 down-regulated and 177 up-regulated proteins were identified, presenting a significantly altered proteome profile of the combined Art and TMP group as compared to the group treated with Art or TMP alone. These results suggested that the Art-TMP combination could be used as a powerful solution for CM and its neurologic damage.ConclusionsAn effictive therapy for CM with low mortality rate and protect against ECM-induced neurocognitive impairment has been proposed through the combination of Art and TMP, which can provide an effective adjuvant treatment in the clinic. iTRAQ proteomics provide a resource for further mechanistic studies to examine the synergistic effects of Art and TMP and their potential to serve as an adjunctive treatment method and intervention targets.Author SummaryCerebral malaria (CM) is the most serious neurological complication caused by Plasmodium falciparum infection. Even after antimalarial treatment, severe neurological sequelae still exist. We used tetramethylpyrazine (TMP), the main ingredient of the traditional Chinese medicine Chuanxiong, and artesunate (Art) as a combination of drugs. We found that Art-TMP combination could improve the clinical symptoms of CM and protect the nervous system. At the same time, proteomics was used to analyze the protective mechanism of Art-TMP combination administration on ECM mice. This study suggests that the combination of Art and TMP may be used as an adjuvant therapy for clinical CM and iTRAQ proteomics provides resources for further study of Art-TMP combination and provides potential prognostic biomarkers for this therapeutic intervention.

show abstract

“…In addition, control (with TSB alone) and negative control (with bacteria alone) were included after incubation at 37°C for 24 h without shaking. The supernatant was removed, the wells were rinsed three times with phosphate-buffered saline (PBS; pH 7.2), 200 μL of 99% methanol was added to the wells to fix the biofilms, and then, the plates were emptied after 15 min and stained for 5 min with 200 μL of 2% crystal violet per well ( Ding et al, 2017 ). The wells were rinsed with PBS (pH 7.2), and the dye was resolubilized with 200 μL of 33% (v/v) glacial acetic acid per well ( Ding et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

“…The supernatant was removed, the wells were rinsed three times with phosphate-buffered saline (PBS; pH 7.2), 200 μL of 99% methanol was added to the wells to fix the biofilms, and then, the plates were emptied after 15 min and stained for 5 min with 200 μL of 2% crystal violet per well ( Ding et al, 2017 ). The wells were rinsed with PBS (pH 7.2), and the dye was resolubilized with 200 μL of 33% (v/v) glacial acetic acid per well ( Ding et al, 2017 ). All wells were then measured using a Tecan GENios Plus microplate reader (Tecan, Austria) at 595 nm ( Ding et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Azithromycin Inhibits Biofilm Formation by Staphylococcus xylosus and Affects Histidine Biosynthesis Pathway

Ding

Zhou

et al. 2018

Front. Pharmacol.

View full text Add to dashboard Cite

Staphylococcus xylosus, a coagulase-negative, non-pathogenic bacterium, responsible for opportunistic infections in humans and bovine mastitis, has the ability to form biofilms, which are responsible for persistent infections and antibiotic resistance. In our study, azithromycin significantly inhibited biofilm formation by altering protein expression. Of the 1764 proteins measured by the isobaric Tag for Relative and Absolute Quantification (iTRAQ) technique, only 148 proteins showed significantly different expression between the azithromycin-treated and untreated cells. Most ribosomal proteins were markedly up-regulated, and the expression of the proteins involved in histidine biosynthesis, which, in turn, influence biofilm formation, was down-regulated, particularly imidazole glycerophosphate dehydratase (IGPD). Previously, we had observed that IGPD plays an important role in biofilm formation by S. xylosus. Therefore, hisB expression was studied by real-time PCR, and the interactions between azithromycin and IGPD were predicted by molecular docking analysis. hisB was found to be significantly down-regulated, and six bond interactions were observed between azithromycin and IGPD. Many active atoms of azithromycin did not interact with the biologically active site of IGPD. Surface plasmon resonance analysis used to further study the relationship between IGPD and azithromycin showed minimum interaction between them. Histidine content in the azithromycin-treated and untreated groups was determined. We noted a slight difference, which was not consistent with the expression of the proteins involved in histidine biosynthesis. Therefore, histidine degradation into glutamate was also studied, and we found that all proteins were down-regulated. This could be the reason why histidine content showed little change between the treated and untreated groups. In summary, we found that azithromycin is a potential inhibitor of S. xylosus biofilm formation, and the underlying mechanism was preliminarily elucidated in this study.

show abstract

Sub-Minimum Inhibitory Concentrations of Rhubarb Water Extracts Inhibit Streptococcus suis Biofilm Formation

Cited by 23 publications

References 45 publications

Intervene effect on Streptococcus suis biofilm formation of emodin extracted from Rheum officinale Baill

Intervene effect on Streptococcus suis biofilm formation of emodin extracted from Rheum officinale Baill

Powerful solution for experimental cerebral malaria treatment: artesunate and tetramethylpyrazine

Azithromycin Inhibits Biofilm Formation by Staphylococcus xylosus and Affects Histidine Biosynthesis Pathway

Contact Info

Product

Resources

About