Quantitative proteomic analysis of ibuprofen-degrading Patulibacter sp. strain I11

Almeida, Barbara; Kjeldal, Henrik; Lolas, Ihab Bishara Yousef; Knudsen, Anders Dahl; Carvalho, Gilda; Nielsen, Kåre Lehmann; Crespo, Maria Teresa Barreto; Stensballe, Allan; Nielsen, Jeppe Lund

doi:10.1007/s10532-012-9610-5

Cited by 63 publications

(44 citation statements)

References 55 publications

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“…Many authors have reported the ability of microorganisms to degrade NSAIDs. A degradative potential has been confirmed for bacterial strains from the genera Bacillus (Marchlewicz et al, 2016), Delftia (De Gusseme et al, 2011), Nocardia (Chen and Rosazza, 1994), Patulibacter (Almeida et al, 2013), Planococcus (Domaradzka et al, 2015), Pseudomonas (Ahmed et al, 2001; De Gusseme et al, 2011; Zhang et al, 2013), Rhodococcus (Ivshina et al, 2006), Sphingomonas (Murdoch and Hay, 2015), Stenotrophomonas (Zhang et al, 2013; Wojcieszyńska et al, 2014), Variovorax (Murdoch and Hay, 2015) and fungal strains from the genera Bjerkandera (Rodarte-Morales et al, 2012), Cunninghamella (Zhong et al, 2003), Ganoderma, Irpex (Marco-Urrea et al, 2010c), Phanerochaete (Hata et al, 2010; Rodarte-Morales et al, 2012), and Trametes versicolor (Marco-Urrea et al, 2010a,b,c; Rodríguez-Rodríguez et al, 2010). …”

Section: Introductionmentioning

confidence: 95%

Variable Effects of Non-steroidal Anti-inflammatory Drugs (NSAIDs) on Selected Biochemical Processes Mediated by Soil Microorganisms

et al. 2016

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Non-steroidal anti-inflammatory drugs (NSAIDs) are the most frequently used group of pharmaceuticals. The high consumption and the uncontrolled disposal of unused drugs into municipal waste or their deposit in landfills can result in an increased concentration of these compounds in soils. Moreover, these drugs can affect the microbial activity. However, there is a lack of knowledge about these effects or it is very limited. Therefore, the objective of this study was to compare the impact of selected commercially available NSAIDs, i.e., diclofenac (DCF), naproxen (NPX), ibuprofen (IBF) and ketoprofen (KTP), applied at concentrations of 1 and 10 mg/kg soil, on the activity of soil microorganisms during the 90-day experiment. To ascertain this impact, substrate-induced respiration (SIR), soil enzyme activities, i.e., dehydrogenase (DHA), acid and alkaline phosphatases (PHOS-H and PHOS-OH) and urease (URE) as well as changes in the rates of nitrification and ammonification processes were determined. In addition, the number of culturable bacteria and fungi were enumerated. In general, the obtained data showed a significant stimulatory effect of NSAIDs on the microbial activity. Higher concentrations of NSAIDs caused a greater effect, which was observed for SIR, PHOS-H, PHOS-OH, URE, N-NO3- and N-NH4+, even during the whole incubation period. Moreover, the number of heterotrophic bacteria and fungi increased significantly during the experiment, which was probably a consequence of the evolution of specific microorganisms that were capable of degrading NSAIDs and used them as an additional source of carbon and energy. However, an inhibitory effect of NPX, IBF or KTP for SIR, DHA, on both phosphatases and culturable bacteria and fungi was observed at the beginning of the experiment. At lower concentrations of NSAIDs, in turn, the effects were negligible or transient. In conclusion, the application of NSAIDs altered the biochemical and microbial activity of soil what may cause the disturbance in soil functioning. It is reasonable to assume that some components of the NSAID formulations could stimulate soil microorganisms, thus resulting in an increase in biochemical activities of the soil.

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Section: Introductionmentioning

confidence: 95%

Variable Effects of Non-steroidal Anti-inflammatory Drugs (NSAIDs) on Selected Biochemical Processes Mediated by Soil Microorganisms

et al. 2016

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“…Almeida et al (2013) investigated the Patulibacter sp. strain I11 isolated from activated sludge to identify the optimal proteins responsible for IBP degradation.…”

Section: Rhizodegradationmentioning

confidence: 99%

Phytoextraction, phytotransformation and rhizodegradation of ibuprofen associated with Typha angustifolia in a horizontal subsurface flow constructed wetland

Zhang

Zhu

et al. 2016

Water Research

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a b s t r a c tWidespread occurrence of trace pharmaceutical residues in aquatic environments is of great concerns due to the potential chronic toxicity of certain pharmaceuticals including ibuprofen on aquatic organisms even at environmental levels. In this study, the phytoextraction, phytotransformation and rhizodegradation of ibuprofen associated with Typha angustifolia were investigated in a horizontal subsurface flow constructed wetland system. The experimental wetland system consisted of a planted bed with Typha angustifolia and an unplanted bed (control) to treat ibuprofen-loaded wastewater (~107.2 mg L À1 ).Over a period of 342 days, ibuprofen was accumulated in leaf sheath and lamina tissues at a mean concentration of 160.7 ng g À1 , indicating the occurrence of the phytoextraction of ibuprofen. Root-uptake ibuprofen was partially transformed to ibuprofen carboxylic acid, 2-hydroxy ibuprofen and 1-hydroxy ibuprofen which were found to be 1374.9, 235.6 and 301.5 ng g À1 in the sheath, respectively, while they were 1051.1, 693.6 and 178.7 ng g À1 in the lamina. The findings from pyrosequencing analysis of the rhizosphere bacteria suggest that the Dechloromonas sp., the Clostridium sp. (e.g. Clostridium saccharobutylicum), the order Sphingobacteriales, and the Cytophaga sp. in the order Cytophagales were most probably responsible for the rhizodegradation of ibuprofen.

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“…The degradation time was extended up to 232 hours for 9 mg L -1 . Almeida et al (2013) described the bacterial strain, Patulibacter sp. I11, which also did not grow on ibuprofen as the sole carbon source.…”

Section: Ibuprofen Degradation In Monosubstrate Culturementioning

confidence: 99%

“…Supplementation of the culture with growth substrates such as glucose, acetate or glutamate is necessary for biomass enhancement, and may activate some non-specifi c enzymes (Greń et al 2010). Nowadays, very few microorganisms have been described as capable of removing ibuprofen in the presence of other carbon sources (Almeida et al 2013, Marco-Urrea et al 2009, Murdoch and Hay 2005.…”

Section: Ibuprofen Degradation In the Presence Of Glucosementioning

confidence: 99%

“…So far, very few bacteria have been described as capable of degrading or biotransforming ibuprofen (Almeida et al 2013, Chen and Rosazza 1994, Murdoch and Hay 2005. Some degradative potential was also found in white-rot fungi such as Trametes versicolor, Irpex lacteus, Ganoderma lucidum, Phanerochaete chrysosporium, Bjerkandera adusta and Bjerkandera sp.…”

Section: Introductionmentioning

confidence: 99%

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Dynamics of ibuprofen biodegradation by Bacillus sp. B1(2015b)

Marchlewicz

Wojcieszyńska

2017

Archives of Environmental Protection

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High intake of over-the-counter, non-steroidal anti-inflammatory drugs, such as ibuprofen, has resulted in their presence in wastewaters and surface waters. The potentially harmful effect of ibuprofen present in the waters has led to a search for new methods of drugs’ removal from the environment. One of the most important technological and economical solutions comprises microbiological degradation of these resistant pollutants. Searching for new strains able to degrade ibuprofen could be one of the answers for increasing the detection of pharmaceuticals in the waters. In this study, the ability of bacterial strain Bacillus thuringiensis B1(2015b) to remove ibuprofen is described. Bacteria were cultured in both monosubstrate and cometabolic systems with 1, 3, 5, 7 and 9 mg L-1 ibuprofen and 1 g L-1 glucose as a carbon source. Bacillus thuringiensis B1(2015b) removed ibuprofen up to 9 mg L-1 in 232 hours in the monosubstrate culture, whereas in the cometabolic culture the removal of the drug was over 6 times faster. That is why the examined strain could be used to enhance the bioremediation of ibuprofen.

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Quantitative proteomic analysis of ibuprofen-degrading Patulibacter sp. strain I11

Cited by 63 publications

References 55 publications

Variable Effects of Non-steroidal Anti-inflammatory Drugs (NSAIDs) on Selected Biochemical Processes Mediated by Soil Microorganisms

Variable Effects of Non-steroidal Anti-inflammatory Drugs (NSAIDs) on Selected Biochemical Processes Mediated by Soil Microorganisms

Phytoextraction, phytotransformation and rhizodegradation of ibuprofen associated with Typha angustifolia in a horizontal subsurface flow constructed wetland

Dynamics of ibuprofen biodegradation by Bacillus sp. B1(2015b)

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