Quantitative Proteomic Analyses To Reveal the Key Features of Proteins in New Onset Ankylosing Spondylitis Patients

Abstract

Ankylosing spondylitis (AS) is a chronic immune-mediated disease. Various immune cells play an essential role in the AS pathogenesis. However, the specific pathogenesis of AS has not been well understood. Proteomic profiles of peripheral blood mononuclear cells (PBMCs) were applied to reveal the specific pathogenesis of AS. Quantitative proteomic analyses were performed using liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based methods to investigate the protein profiling of PBMCs from new-onset AS patients ( n = 9) and healthy controls ( n = 9). We identified 782 differentially expressed proteins (DEPs) and 527 differentially phosphorylated proteins (DPPs) between AS patients and healthy controls. The subcellular location of DEPs and DPPs showed that most of the DEPs were from the cytoplasm ( n = 296, 38%), were extracellular ( n = 141, 18%), and from the nucleus ( n = 114, 15%); most of the DPPs were from the cytoplasm ( n = 37, 34%), nucleus ( n = 35, 32%), and plasma membrane ( n = 10, 9%). We further identified 89 proteins with both expression and phosphorylation differences. The functional annotation of the 89 differentially expressed and phosphorylated proteins enriched in the antigen processing and presentation pathway. Four DEPs with six phosphorylated positions were found in the antigen processing and presentation pathway. The differentially expressed and phosphorylated proteins may be helpful to uncover the pathogenesis of AS. The six AS-specific proteins may serve as candidate markers for AS diagnosis and new treatment targets.

Systematic proteomics analysis of lysine acetylation reveals critical features of placental proteins in pregnant women with preeclampsia

“…Previous researchers have used iTRAQ proteomics to identify differentially expressed proteins (DEPs) in PE and normal pregnancy placental tissues to look for potential biomarkers of PE 6 . Quantitative analysis and PTMs of proteins are essential for understanding biological complexity 12,18 . To better understand the pathogenesis of PE, we integrated proteome and acetyl proteome (acetylome) systematically to analyse the vital features of placental proteins in pregnant women with PE and provided a landscape of acetylation in PE, which may contribute to developing valuable novel biomarkers for PE.…”

Global Proteomic Analyses Reveals Abnormal Immune Regulation in Patients With New Onset Ankylosing Spondylitis

“…The clinical and laboratory results between AS patients and healthy controls have been reported in our previous study ( 16 ).…”

“…The expression of HLA-B27 is highly linked with the presence of AS ( 38 , 39 ), and other genes related to AS have been discovered ( 40 , 41 ). In our previous studies, we identified differences in the expression and phosphorylation of HLA-E, calnexin (CANX), and two heat-shock proteins (HSPs) (HSP90AA1 and HSP90AB1) in PBMCs of AS patients and healthy controls ( 16 ). In the present study, we further verified these proteins by PRM and demonstrated that HSP90AA1 and HSP90AB1 were highly expressed, indicating that HSP90AA1 and HSP90AB1 may be involved in the development of AS.…”

“…The clinical information of the participants has been reported in our previous study ( 16 ). This observational study was conducted at the Department of Nephrology, The Affiliated Hospital of Jinan University (Guangzhou, China).…”