Systematic proteomics analysis of lysine acetylation reveals critical features of placental proteins in pregnant women with preeclampsia

Abstract

Preeclampsia (PE) is a dangerous hypertensive disorder that occurs during pregnancy. The specific aetiology and pathogenesis of PE have yet to be clarified. To better reveal the specific pathogenesis of PE, we characterized the proteome and acetyl proteome (acetylome) profile of placental tissue from PE and normal‐term pregnancy by label‐free quantification proteomics technology and PRM analysis. In this research, 373 differentially expressed proteins (DEPs) were identified by proteome analysis. Functional enrichment analysis revealed significant enrichment of DEPs related to angiogenesis and the immune system. COL12A1, C4BPA and F13A1 may be potential biomarkers for PE diagnosis and new therapeutic targets. Additionally, 700 Kac sites were identified on 585 differentially acetylated proteins (DAPs) by acetylome analyses. These DAPs may participate in the occurrence and development of PE by affecting the complement and coagulation cascades pathway, which may have important implications for better understand the pathogenesis of PE. In conclusion, this study systematically analysed the reveals critical features of placental proteins in pregnant women with PE, providing a resource for exploring the contribution of lysine acetylation modification to PE.

Integrated proteome and acetylome analyses unveil protein features of gestational diabetes mellitus and preeclampsia

“…GSEA results revealed that the complement and coagulation cascades pathway was up-regulated in the proteome and acetylome analyses of placental tissues for GDM and PE pregnant women. Many studies demonstrate the pathological effect of the complement and coagulation cascades pathway in GDM and PE [13,21,22]. The significantly regulated proteins present only in the GDM group were mainly enriched in the phospholipid metabolic pathway.…”

“…All pregnant women with GDM meet ADA diagnostic criteria [19]. Exclusion criteria for all pregnant women were consistent with our previous study [13]. The study was approved by the Medical Ethics Committee of Shenzhen People's Hospital, and written informed consent was obtained from all participants.…”

“…We divide 18 placental tissue samples into three groups: the GDM group, PE group, and control group. The proteome and acetylome analysis between placental tissues from PE patients and control groups has been studied previously [13]. Herein, we further explored the characteristics of proteome and acetylome among GDM, PE, and control groups.…”

“…Enhanced histone acetylation has been found in the disease process of GDM and PE [11,12]. Shangguan et al [13] characterized the acetylome profile of placental tissue from PE. In addition, studies have described the modulation of PE-related histone acetylation as a molecular therapeutic target [14,15].…”

Integrated Proteomic and N-Glycoproteomic Profiling of Placental Tissues of Patients with Preeclampsia

“… 23 , 24 For instance, Tang et al used label-free quantification proteomic technology to compare placental proteomes of normal-term pregnant women and PE patients, and found that DEPs were involved in multiple biological processes associated with immunity and angiogenesis. 25 In the present study, 2260 highly reliable proteins were identified in nine placental tissues, and 60 proteins were differentially expressed between PE and normal placentas. These altered proteins are mainly enriched in biological processes, such as platelet degranulation, cell migration and adhesion, blood coagulation, and redox process, which are essential for the development of placenta.…”