Quantitative protein changes in metastatic versus primary epithelial ovarian carcinoma

Lawson, Stephen R.; Latter, G; Miller, David S.; Goldstein, David J.; Naps, Michelle; Burbeck, Stephen; Teng, Nelson N.H.; Zuckerkandl, Emile

doi:10.1016/0090-8258(91)90248-4

Cited by 7 publications

(2 citation statements)

References 20 publications

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“…For each distinct field, novel technologies and methodologies have provided important insights. However, exciting and emerging technologies such as in vivo microscopy of cell motility (Farina et al, ) or proteomics combined with laser capture microdissection (Lawson et al, ; Emmert‐Buck et al, ; see UNIT here) will provide valuable insights into the field. For example, it is feasible to microdissect patient‐matched normal, tumor, and metastatic tissue cells and analyze their corresponding DNA, mRNA, and protein levels, giving a true cellular snapshot.…”

Section: Resultsmentioning

confidence: 99%

Overview of Metastasis Assays

2001

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During tumor progression, cells acquire genetic and proteomic changes as they transform from normal to hyperplastic, through dysplasia, to carcinoma in situ, and finally to invasive and metastatic. The time course of progression may extend as far back as 10 years prior to diagnosis. Discerning the mechanism whereby tumor cells execute metastatic dissemination may provide the foundation necessary for successful treatment of the disease. For example, direct genetic evidence has linked in situ breast cancer to invasive carcinoma of the breast supporting the generally accepted assumption that carcinoma in situ of the breast is a clonal expansion of hyperproliferating cells. This in turn may provide a more comprehensive and/or functionally directed target strategy for intervention and prevention of breast cancer. This overview provides a picture of the processes related to metastasis and the experimental approaches used to study these processes.

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Section: Resultsmentioning

confidence: 99%

Overview of Metastasis Assays

2001

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“…In the past, many investigators have analyzed lysates from cell lines [25][26][27][28] and human tissue [29][30][31][32][33][34][35] by 2D-PAGE to look at tumor specific alterations in protein expression for new marker and target discovery. Image databases were developed to map proteins expressed in specific cell types and at defined stages of tumor progression [36][37][38].…”

mentioning

confidence: 99%

Cancer Proteomics: The State of the Art

2001

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Now that the human genome has been determined, the field of proteomics is ramping up to tackle the vast protein networks that both control and are controlled by the information encoded by the genome. The study of proteomics should yield an unparalleled understanding of cancer as well as an invaluable new target for therapeutic intervention and markers for early detection. This rapidly expanding field attempts to track the protein interactions responsible for all cellular processes. By careful analysis of these systems, a detailed understanding of the molecular causes and consequences of cancer should emerge. A brief overview of some of the cutting edge technologies employed by this rapidly expanding field is given, along with specific examples of how these technologies are employed. Soon cellular protein networks will be understood at a level that will permit a totally new paradigm of diagnosis and will allow therapy tailored to individual patients and situations.

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An approach to proteomic analysis of human tumors

et al. 2000

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A strategy for proteomic analysis of microdissected cells derived from human tumor specimens is described and demonstrated by using esophageal cancer as an example. Normal squamous epithelium and corresponding tumor cells from two patients were procured by laser-capture microdissection and studied by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Fifty thousand cells resolved approximately 675 distinct proteins (or isoforms) with molecular weights ranging between 10 and 200 kDa and isoelectric points of pH 3-10. Comparison of the microdissected protein profiles showed a high degree of similarity between the matched normal-tumor samples (98% identical). However, 17 proteins showed tumor-specific alterations, including 10 that were uniquely present in the tumors and seven that were observed only in the normal epithelium. Two of the altered proteins were characterized by mass spectrometry and immunoblot analysis and were identified as cytokeratin 1 and annexin I. Acquisition of 2D-PAGE protein profiles, visualization of disregulated proteins, and subsequent determination of the identity of selected proteins through high-sensitivity MS-MS microsequencing are possible from microdissected cell populations. These separation and analytical techniques are uniquely capable of detecting tumor-specific alterations. Continued refinement of techniques and methodologies to determine the abundance and status of proteins in vivo holds great promise for future study of normal cells and associated neoplasms. Mol.

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Quantitative protein changes in metastatic versus primary epithelial ovarian carcinoma

Cited by 7 publications

References 20 publications

Overview of Metastasis Assays

Overview of Metastasis Assays

Cancer Proteomics: The State of the Art

An approach to proteomic analysis of human tumors

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