Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation

Dondero, Francesco; Piacentini, Luciana; Bannı, Mohamed; Rebelo, Mauro de Freitas; Burlando, Bruno; Viarengo, Aldo

doi:10.1016/j.gene.2004.11.031

Cited by 161 publications

(90 citation statements)

References 29 publications

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“…Recently, gene expression has been acknowledged as an effective approach to biomonitor environmental contamination in many marine mollusks (Boutet et al, 2003;Dondero et al, 2005;Wan et al, 2008bWan et al, , 2009. Based on the mRNA expression profi le data, it was possible to estimate chemical exposure and evaluate the risk of exposed contaminants (Lee et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Zhang

Liu

et al. 2011

Chin. J. Ocean. Limnol.

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Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxifi cation of xenobiotics, and also play important roles in antioxidant defense. We identifi ed two glutathione S-transferase isoforms (VpGSTS, sigma GST; VpGSTO, omega GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp, encoding 203 and 242 amino acids with an estimated molecular mass of 22.88 and 27.94 kDa, respectively. The expression profi les of VpGSTS and VpGSTO responded to heavy metals and benzo[a]pyrene (B[a]P) exposure were investigated by quantitative real-time RT-PCR. The expression of VpGSTS and VpGSTO were both rapidly up-regulated, however, they showed differential expression patterns to different toxicants. Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum 6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For B[a]P exposure, the maximum induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether, these fi ndings implied the involvement of VpGSTS and VpGSTO in host antioxidant responses, and highlighted their potential as a biomarker to Cd and B[a]P exposure.

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Section: Discussionmentioning

confidence: 99%

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Zhang

Liu

et al. 2011

Chin. J. Ocean. Limnol.

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“…MTs play a role in protection against oxidative stress in different organisms (Vergani et al 2005);14 in Mytilus, the MT-20 isoform is preferentially induced by oxidant agents (such as hydrogen peroxide) and cadmium, whereas MT-10 is the constitutive isoform that preferentially responds to essential metals, such as zinc (Lemoine and Laulier, 2003;Dondero et al, 2005). The effects of BPA were distinct from those previously observed in the same experimental conditions with E 2 , that induced significant increases in the expression of both catalase and MT20 (Canesi et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Bisphenol-A alters gene expression and functional parameters in molluscan hepatopancreas

Canesi

Borghi

Ciacci

et al. 2007

Molecular and Cellular Endocrinology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.A c c e p t e d M a n u s c r i p t A c c e p t e d M a n u s c r i p t 2 AbstractBisphenol A (BPA) is a well-known xenoestrogen in mammalian systems that can affect reproduction also in aquatic organisms. In this work the possible effects of BPA were investigated in the hepatopancreas of the bivalve mollusc Mytilus galloprovincialis: mussels were injected with different amounts of BPA (3-60 ng/g dw tissue) and tissues sampled at 24 h post-injection. Overall, these data indicate that BPA, at environmentally relevant concentrations, can have both estrogen-like and distinct effects in invertebrates like in vertebrates. Expression of different

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“…It also has been proposed that different MT isoforms possibly carry out distinct functions during the processes of metal homeostasis and detoxification (Sadhu and Gedamu, 1988;Dondero et al, 2005). In mammals, MTs are speculated to be the predominant single liganding species for Zn in cells that have not been treated with other heavy metals, and they can release Zn in response to induction with Cd or Cu (Suzuki et al, 1990).…”

Section: Induction Of Tpig-mt1 Transcription By Heavy Metals and Hydrmentioning

confidence: 99%

“…In animals, multiple MT genes with distinct biological properties have been demonstrated in different tissues, but it is not clear what important physiological functions they possess (Palmiter, 1998). Invertebrate species have also been shown to have multiple isoforms of MTs, which can exhibit differential expression and regulation (Dondero et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Cloning, characterization, and gene expression analysis of a novel cadmium metallothionein gene in Tetrahymena pigmentosa

Guo

Miao

2008

Gene

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Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation

Cited by 161 publications

References 29 publications

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Bisphenol-A alters gene expression and functional parameters in molluscan hepatopancreas

Cloning, characterization, and gene expression analysis of a novel cadmium metallothionein gene in Tetrahymena pigmentosa

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