Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Abstract

Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxifi cation of xenobiotics, and also play important roles in antioxidant defense. We identifi ed two glutathione S-transferase isoforms (VpGSTS, sigma GST; VpGSTO, omega GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp, encoding 203 and 242 amino acids with an estimated molecular mass of 22.88 and 27.94 kDa, respectively. The expression profi les of VpGSTS and VpGSTO responded to heavy metals and benzo[a]pyrene (B[a]P) exposure were investigated by quantitative real-time RT-PCR. The expression of VpGSTS and VpGSTO were both rapidly up-regulated, however, they showed differential expression patterns to different toxicants. Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum 6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For B[a]P exposure, the maximum induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether, these fi ndings implied the involvement of VpGSTS and VpGSTO in host antioxidant responses, and highlighted their potential as a biomarker to Cd and B[a]P exposure.

Transcriptional response of four C1q domain containing protein (C1qDC) genes from Venerupis philippinarum exposed to the water soluble fraction of No.0 diesel oil

“…Liu et al, 2014). More or less compatible transcriptional modulation patterns were also observed in our previous studies (C. Li et al, 2011;Zhang et al, 2012b).…”

Molecular Cloning and Expression of MnGST-1 and MnGST-2 from Oriental River Prawn, Macrobrachium nipponense, in Response to Hypoxia and Reoxygenation

“…Delta and Epsilon groups are only present in insect species, and Omega, Theta, Sigma, and Zeta, while microsomal groups are also present in other species. The GSTs that have been well characterized in Venerupis philippinarum are belong to Omega and Sigma class [ 20 ], while the GST that has been characterized in Litopenaeus vannamei belongs to the Mu class [ 17 ]. However, GSTs in oriental river prawn, Macrobrachium nipponense , have not been investigated.…”