Transcriptional changes in oysters  Crassostrea brasiliana  exposed to phenanthrene at different salinities

Zacchi, Flávia L.; Lima, Daína; Flores-Nunes, Fabrício; Mattos, Jacó Joaquim; Lüchmann, Karim Hahn; Gomes, Carlos Henrique Araújo de Miranda; Bı́cego, Márcia Caruso; Taniguchi, Satie; Sasaki, Silvio Tarou; Bainy, Afonso Celso Dias

doi:10.1016/j.aquatox.2016.12.016

Cited by 28 publications

(11 citation statements)

References 86 publications

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“…NPR1 has been found to be putatively involved in kidney and brain sodium excretion responses in mice and ducks [48,49] and was strongly associated with abiotic environmental variation in three coastal populations (FRY, Woolnorth and Forestier) in a previous landscape genomics study [21]. Other genes in this module have been implicated in living in variable salinity environments, including FOXA1 [50], MGST1 [51], and TSPAN13 [52]. These findings may reflect a greater role for salinity as an environmental pressure in coastal environments than previously appreciated.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics of Tasmanian Devil (Sarcophilus Harrisii) Ear Tissue Reveals Homogeneous Gene Expression Patterns across a Heterogeneous Landscape

Fraik

Quackenbush

Margres

et al. 2019

Genes

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In an era of unprecedented global change, exploring patterns of gene expression among wild populations across their geographic range is crucial for characterizing adaptive potential. RNA-sequencing studies have successfully characterized gene expression differences among populations experiencing divergent environmental conditions in a wide variety of taxa. However, few of these studies have identified transcriptomic signatures to multivariate, environmental stimuli among populations in their natural environments. Herein, we aim to identify environmental and sex-driven patterns of gene expression in the Tasmanian devil (Sarcophilus harrisii), a critically endangered species that occupies a heterogeneous environment. We performed RNA-sequencing on ear tissue biopsies from adult male and female devils from three populations at the extremes of their geographic range. There were no transcriptome-wide patterns of differential gene expression that would be suggestive of significant, environmentally-driven transcriptomic responses. The general lack of transcriptome-wide variation in gene expression levels across the devil’s geographic range is consistent with previous studies that documented low levels of genetic variation in the species. However, genes previously implicated in local adaptation to abiotic environment in devils were enriched for differentially expressed genes. Additionally, three modules of co-expressed genes were significantly associated with either population of origin or sex.

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Section: Discussionmentioning

confidence: 99%

Transcriptomics of Tasmanian Devil (Sarcophilus Harrisii) Ear Tissue Reveals Homogeneous Gene Expression Patterns across a Heterogeneous Landscape

Fraik

Quackenbush

Margres

et al. 2019

Genes

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“…salinity stress, but were down-regulated under long-term salinity stress in white shrimp (Gao et al 2012). Thirty-three salinity-and oxidative stress-induced genes, including LEA2, HSPs, GST, coagulation factor genes, and P450 enzyme, were differentially expressed in Haliotis discus, Eriocheir sinensis megalopae, and Crassostrea brasiliana (DeZoysa et al 2009;Hui et al 2014;Zacchi et al 2017). The Na + /K + -ATPase (NKA) α-subunit, intracellular fatty acid-binding protein, and innexin 2 showed the highest gene expression levels under low-salinity stress in the shrimp Penaeus monodon (Shekhar et al 2013).…”

Section: Introductionmentioning

confidence: 98%

Salinity stress-induced differentially expressed miRNAs and target genes in sea cucumbers Apostichopus japonicus

Tian

Shang

Guo

et al. 2019

Cell Stress and Chaperones

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Environmental salinity is an important abiotic factor influencing normal physiological functions and productive performance in the sea cucumber Apostichopus japonicus. It is therefore important to understand how changes in salinity affect sea cucumbers in the face of global climate change. In this study, we investigated the responses to salinity stress in sea cucumbers using mRNA and miRNA sequencing. The regulatory network of mRNAs and miRNAs involved in salinity stress was examined, and the metabolic pathways enriched for differentially expressed miRNAs and target mRNAs were identified. The top 20 pathways were involved in carbohydrate metabolism, fatty acid metabolism, degradation, and elongation, amino acid metabolism, genetic information processing, metabolism of cofactors and vitamins, transport and catabolism, and environmental information processing. A total of 22 miRNAs showed differential expression during salinity acclimation. The predicted 134 target genes were enriched in functions consistent with the results of gene enrichment based on transcriptome analysis. These results suggested that sea cucumbers deal with salinity stress via changes in amino acid metabolism, ion channels, transporters, and aquaporins, under stimulation by environmental signals, and that this process requires energy from carbohydrate and fatty acid metabolism. Salinity challenge also induced miRNA expression. These results provide a valuable genomic resource that extends our understanding of the unique biological characteristics of this economically important species under conditions of salinity stress.

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“…In a thus far rare experiment testing the effects of salinity on Phe exposure on C. brasiliana, strictly increasing bioaccumulation was not observed with decreasing salinity (Zacchi et al, 2017). Instead, the intermediate salinity ( 25) accumulated the highest amount of Phe (88.4 µg/g dry weight) followed by low salinity (10, 81.5 µg/g dry weight) and then high salinity (35, 72.0 µg/g dry weight) (Zacchi et al, 2017).…”

Section: Salinity and Pahsmentioning

confidence: 91%

“…However, low salinities still carry the burden of hypoosmotic stress or other mechanisms that negatively affect the health of oysters. The intermediate amount of Phe accumulated at the lowest salinity was associated with elevated CYP isoforms (CYP2AU1 and CYP2-like1) after 24 h of exposure with high levels of CYP2-like1 remaining after 96 h when compared to the control (Zacchi et al, 2017). As CYP proteins are involved in stage I metabolism of PAHs, this shows an increased effort to detoxify Phe at low salinities, possibly due to increased toxicity.…”

Section: Salinity and Pahsmentioning

confidence: 91%

“…As a result, the antioxidant enzymes CAT-like, SOD-like, and two GST proteins GSTm-like and GST -like were upregulated in the low salinity treatment compared to the two higher salinity treatments as expected. Other than oxidative stress; however, Phe did not seem to inhibit osmoregulation activities and gene expression since amino acidmetabolizing proteins were upregulated with no impact from Phe (Zacchi et al, 2017).…”

Section: Salinity and Pahsmentioning

confidence: 91%

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Impact of Polycyclic Aromatic Hydrocarbon Accumulation on Oyster Health

Gan

Martin

2021

Front. Physiol.

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In the past decade, the Deepwater Horizon oil spill triggered a spike in investigatory effort on the effects of crude oil chemicals, most notably polycyclic aromatic hydrocarbons (PAHs), on marine organisms and ecosystems. Oysters, susceptible to both waterborne and sediment-bound contaminants due to their filter-feeding and sessile nature, have become of great interest among scientists as both a bioindicator and model organism for research on environmental stressors. It has been shown in many parts of the world that PAHs readily bioaccumulate in the soft tissues of oysters. Subsequent experiments have highlighted the negative effects associated with exposure to PAHs including the upregulation of antioxidant and detoxifying gene transcripts and enzyme activities such as Superoxide dismutase, Cytochrome P450 enzymes, and Glutathione S-transferase, reduction in DNA integrity, increased infection prevalence, and reduced and abnormal larval growth. Much of these effects could be attributed to either oxidative damage, or a reallocation of energy away from critical biological processes such as reproduction and calcification toward health maintenance. Additional abiotic stressors including increased temperature, reduced salinity, and reduced pH may change how the oyster responds to environmental contaminants and may compound the negative effects of PAH exposure. The negative effects of acidification and longer-term salinity changes appear to add onto that of PAH toxicity, while shorter-term salinity changes may induce mechanisms that reduce PAH exposure. Elevated temperatures, on the other hand, cause such large physiological effects on their own that additional PAH exposure either fails to cause any significant effects or that the effects have little discernable pattern. In this review, the oyster is recognized as a model organism for the study of negative anthropogenic impacts on the environment, and the effects of various environmental stressors on the oyster model are compared, while synergistic effects of these stressors to PAH exposure are considered. Lastly, the understudied effects of PAH photo-toxicity on oysters reveals drastic increases to the toxicity of PAHs via photooxidation and the formation of quinones. The consequences of the interaction between local and global environmental stressors thus provide a glimpse into the differential response to anthropogenic impacts across regions of the world.

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Transcriptional changes in oysters Crassostrea brasiliana exposed to phenanthrene at different salinities

Cited by 28 publications

References 86 publications

Transcriptomics of Tasmanian Devil (Sarcophilus Harrisii) Ear Tissue Reveals Homogeneous Gene Expression Patterns across a Heterogeneous Landscape

Transcriptomics of Tasmanian Devil (Sarcophilus Harrisii) Ear Tissue Reveals Homogeneous Gene Expression Patterns across a Heterogeneous Landscape

Salinity stress-induced differentially expressed miRNAs and target genes in sea cucumbers Apostichopus japonicus

Impact of Polycyclic Aromatic Hydrocarbon Accumulation on Oyster Health

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