Quantitative Methods for the Analysis of Hemicellulose in Apple Wood.

Tottingham, W. E.; Gerhardt, Fisk

doi:10.1021/ie50170a013

Cited by 3 publications

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“…H2SO4 and refluxed for 2 hours. The clarified hydrolyzates from both pro- (31) has reported the presence of polyhexosans in the base wood of the apple. His technique, however, did not include the removal of the pectic constituents prior to acid hydrolysis.…”

Section: Carbohydrate Recovery In the Acid And Alkaline Hydrolyzatesmentioning

confidence: 99%

Effect of Acid and Alkaline Hydrolysis on the Estimation of Hemicellulose and Associated Groups in Young Apple Wood

Gerhardt¹

1929

Plant Physiol.

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Section: Carbohydrate Recovery In the Acid And Alkaline Hydrolyzatesmentioning

confidence: 99%

Effect of Acid and Alkaline Hydrolysis on the Estimation of Hemicellulose and Associated Groups in Young Apple Wood

Gerhardt¹

1929

Plant Physiol.

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“…Although HORTON (12) questions its reliability in some cases, it is generally conceded to be a trustworthy and convenient hydrolysant for analytical work. Salivary diastase was used by TOTTINGHAM and GERHARDT (23). The most commonly used source of diastase is barley malt.…”

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“…(22). With some tissues, however, sulphuric acid seems to be preferable (23). The length of time of hydrolysis varies, but is usually from 1 to 4 hours.…”

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“…The first, or phloroglucinol method, is admittedly subject to many errors; but the only improvement on it, the method of PERVIER and GORTNER (17), is rather cumbersome to carry out. The fermentation method has been apparently successful in the hands of several investigators (5,21,23). This method is based upon the assumption that pentoses are not destroyed by yeast.…”

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The Determination of Polysaccharides

Appleman¹,

Loomis²,

Phillips³

et al. 1927

Plant Physiol.

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Determination of Starch in Plant Tissues

Shriner

1932

Plant Physiol.

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IntroductionDuring a study of the carbohydrate distribution in grape canes, it became necessary to have a method for the estimation of small amounts of starch. A fairly rapid, accurate method yielding easily reproducible results was desired in order that the analysis of a great number of samples could be completed in a short time.An excellent survey of the literature dealing with analytical methods for starch is given by ANTOLTON (11). Of all the methods listed, one described by THOMAS (9) for estimating starch in apple spurs seemed most suitable. The fundamental idea of THOMAS'S method is the direct determination of the combined reducing power of glucose and maltose produced by the action of diastase on starch under prescribed conditions. This method avoids the destruction of sugars which always takes place if complete hydrolysis with acids is attempted or if acid hydrolysis after diastatic conversion of the starch is used according to the Official Methods (6). This fact was shown by THOMAS and confirmed in the present work. THOMAS determined the reducing sugars by means of the calorimetric method of BENEDICT and OSTER-BERG (1), which involved the reduction of a picrate-picric acid solution to picramic acid. When this method was tested out on pure starch it always yielded high results (table I). The use of mercuric nitrate as a clarifying agent was also unsatisfactory since it yielded a precipitate which was slow in settling and difficult to filter. Moreover, mercuric nitrate exerts an oxidizing influence on sugar solutions (5). It is evident that the clarifying agent must be chosen to suit the particular type of substrate obtained from the different plants.The procedure which was found most satisfactory is as follows:

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Quantitative Methods for the Analysis of Hemicellulose in Apple Wood.

Cited by 3 publications

References 0 publications

Effect of Acid and Alkaline Hydrolysis on the Estimation of Hemicellulose and Associated Groups in Young Apple Wood

Effect of Acid and Alkaline Hydrolysis on the Estimation of Hemicellulose and Associated Groups in Young Apple Wood

The Determination of Polysaccharides

Determination of Starch in Plant Tissues

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