Quantitative measurement of IgE antibodies to purified allergens using streptavidin linked to a high-capacity solid phase

Erwin, Elizabeth A.; Custis, N.J.; Satinover, S.M.; Perzanowski, Matthew S.; Woodfolk, Judith A.; Crane, Julian; Wickens, Kristin; Platts-Mills, Thomas A.E.

doi:10.1016/j.jaci.2004.12.1131

Cited by 94 publications

(89 citation statements)

References 29 publications

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“…In other words, the use of 'customized', properly balanced selection of allergenic molecules in IgE assays will be a valuable compromise between 'tradition' and 'modernity' in serological in vitro allergy testing and the best strategy to harmonize 'Precision Medicine' with a 'Choosing Wisely' approach in the field of allergology. The levels of IgE to an extract of D. pteronyssinus are underestimated in highly sensitized patients, due to a limited binding capacity of the extract [20] …”

Section: Discussionmentioning

confidence: 99%

Molecular Allergology between Precision Medicine and the Choosing Wisely initiative

Matricardi

Kleine-Tebbe²

2016

Clin Experimental Allergy

115

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Section: Discussionmentioning

confidence: 99%

Molecular Allergology between Precision Medicine and the Choosing Wisely initiative

Matricardi

Kleine-Tebbe²

2016

Clin Experimental Allergy

115

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“…They could have a significant impact on the efficacy of immunotherapy with Fel d 1 alone and may need to be considered for use in patient selection for therapy. This consideration has become increasingly relevant since it is now evident that most cat-allergic adults with rhinoconjunctivitis have low titers of anti-Fel d 1 antibody, so even small IgE responses to other specificities could constitute a significant proportion of their allergy [5,6,7,8,9,10,11]. This study describes two newly identified cat allergens of potential importance.…”

Section: Introductionmentioning

confidence: 99%

Two Newly Identified Cat Allergens: The von Ebner Gland Protein Fel d 7 and the Latherin-Like Protein Fel d 8

Smith

O’Neil

Hales

et al. 2011

Int Arch Allergy Immunol

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Introduction: Characterization of the complete IgE binding spectrum of cat allergens is important for the development of improved diagnosis and effective immunotherapeutics. While Fel d 1 remains unchallenged as the major cat allergen, we now report the isolation of two new allergens capable of binding similar concentrations of IgE in the allergic sera of some individuals. Materials and Methods: Cat tongue and submandibular salivary gland cDNA libraries were screened by DNA hybridisation and IgE immunoassay. The isolated DNA fragments were sub-cloned into an E. coli expression system and the IgE reactivity was examined with human cat-allergic sera using a DELFIA IgE quantitation assay. Results: Fel d 7, an 18 kDa von Ebner gland protein Can f 1 homologue, was isolated from the tongue library. Fel d 8, a 24-kDa latherin-like protein with homology to Equ c 5, was isolated from the submandibular library. The frequency of IgE binding of cat-allergic sera to recombinant Fel d 1, 7 and 8 was 60.5, 37.6 and 19.3%, respectively. Inhibition studies indicated some IgE binding cross-reactivity between Fel d 7 and dog dander extracts. Discussion: The study reports the isolation and characterization of two new cat allergens. The isolation of these allergens provides the opportunity to determine the role that IgE binding proteins other than Fel d 1 play in cat-allergic disease. For cat-allergic individuals with moderate to mild rhinoconjunctivitis these allergens may play a more important role in the manifestation of their allergic disease.

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“…A similar assay was also developed (adapting the method of Erwin et al 2005) in order to compare the reaction of the above subset of 73 individuals, having raised IgE to wheat, against in-house prepared antigens from Australian wheat and spelt (GWF spelt). The analysis of the Australian derived antigens was optimized by coupling the soluble antigens with streptavidin beads and assayed using the Phadia ImmunoCAP 100 machine.…”

Section: Determination Of Ige Levels By Phadia Immunocap 100mentioning

confidence: 99%

The prevalence of wheat and spelt sensitivity in a randomly selected Australian population

Vu¹,

Chin²,

Pasco³

et al. 2015

Cereal Research Communications

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To determine the prevalence of wheat sensitivity in a randomly selected Australian population, a study was carried out on sera samples of the Geelong Osteoporosis Study (GOS) age-stratified cohorts of men and women who were randomly selected from electoral rolls for the Barwon Statistical Division (n = 1145). The human sera were analysed by ELISA-based method using the ImmunoCAP 100 instrument (Phadia, Sweden). IgE from human sera were bound to the wheat (f4) and milk (f2) allergens that have been previously coated on the ImmunoCAPs. The number of IgE wheat and milk RAST positive individuals was determined. In order to relate the increased IgE immuno-reactivity to allergen symptoms, a questionnaire was established and sent to the blood donors and 974 individuals responded. Of these (n = 974) 147 individuals (15.1%) reported symptoms caused by wheat consumption, 179 (15.6%) and 112 (9.8%) sera showed RAST positive results (IgE > 60 response units) in wheat and milk RAST tests, respectively. However, only 2.5% of those participants with symptoms related to wheat had positive IgE values indicating that the relationship is complex: a large proportion (12.7%) of the investigated population might suffer from other wheat related disorders (i.e. not IgE mediated), such as celiac disease, non-celiac reaction to gluten, reaction to fructans for those with irritable bowel syndrome as well as other factors. For the 13.2% who showed raised IgE antibody levels without symptoms we postulate that these individuals have latent wheat sensitivity with the potential of developing symptoms sooner or later.A comparative study to investigate the immune reactivity of human IgE against wheat and spelt antigens was carried out using the sera of 73 patients found to be RAST positive for wheat. Of these 63% (n = 50) showed a higher IgE immune reactivity against wheat, while 30% (n = 24) showed higher IgE response against spelt antigens, the remaining 7% have indifferent responses against both antigens. Since the provided Phadia wheat and spelt antigens used in this study originated from Europe, Australian wheat and spelt varieties were also used to prepare antigens in order to investigate the response of Australian sera to local wheats. It was found that the immune reactivity of IgE wheat positive sera from a normal Australian population is lower for spelts compared to wheats regardless of their origin but much lower against an Australian spelt containing a mutation in its expansin gene. A clinical feeding trial would be

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Quantitative measurement of IgE antibodies to purified allergens using streptavidin linked to a high-capacity solid phase

Cited by 94 publications

References 29 publications

Molecular Allergology between Precision Medicine and the Choosing Wisely initiative

Molecular Allergology between Precision Medicine and the Choosing Wisely initiative

Two Newly Identified Cat Allergens: The von Ebner Gland Protein Fel d 7 and the Latherin-Like Protein Fel d 8

The prevalence of wheat and spelt sensitivity in a randomly selected Australian population

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