Molecular Allergology between Precision Medicine and the Choosing Wisely initiative

Matricardi, Paolo Maria; Kleine-Tebbe, J.

doi:10.1111/cea.12679

Cited by 52 publications

(127 citation statements)

References 20 publications

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“…d) suggests a close relationship, suitable for individual prediction with the collected data. However, results of sensitization tests like the allergen‐specific IgE assays cannot yet (and presumably never will) reliably predict clinical reactions (or their non‐appearance) . Therefore, future studies on the diagnostic value of allergenic molecules should establish as their primarily goal, the improvement of well‐defined methodological variables (Table , left column) that are linked to the analytical performance characteristics of the assay.…”

Section: Clinical Evaluation: Diagnostic Sensitivity and Specificitymentioning

confidence: 99%

“…Even in this era with the availability of molecular allergens, the basic rule still applies. Namely, a positive allergen‐specific IgE result represents a state of allergic sensitization (risk for allergic disease), but not proof of allergic disease . A positive IgE antibody response is only clinically relevant in the case that there are objectively defined corresponding allergic symptoms that are temporally associated with a known allergen exposure.…”

Section: Determination Of the Clinical Relevance Of An Ige Antibody Amentioning

confidence: 99%

“…In conclusion, the clinical relevance of an allergic sensitization (i.e., presence of allergen‐specific IgE) independent of the use of allergen extracts or molecules for diagnostic purposes can ultimately only be determined by the physician and not by the test . Therefore, the complete diagnostic results of sensitizations tests, including allergen‐specific IgE assays that have been improved by the use of allergenic molecules, will always have to be interpreted in the clinical context and on the basis of the individual's case history.…”

Section: Determination Of the Clinical Relevance Of An Ige Antibody Amentioning

confidence: 99%

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EAACI Molecular Allergology User's Guide

Matricardi

Kleine-Tebbe²,

Hoffmann

et al. 2016

Pediatric Allergy Immunology

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724

870

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The availability of allergen molecules ('components') from several protein families has advanced our understanding of immunoglobulin E (IgE)-mediated responses and enabled 'component-resolved diagnosis' (CRD). The European Academy of Allergy and Clinical Immunology (EAACI) Molecular Allergology User's Guide (MAUG) provides comprehensive information on important allergens and describes the diagnostic options using CRD. Part A of the EAACI MAUG introduces allergen molecules, families, composition of extracts, databases, and diagnostic IgE, skin, and basophil tests. Singleplex and multiplex IgE assays with components improve both sensitivity for low-abundance allergens and analytical specificity; IgE to individual allergens can yield information on clinical risks and distinguish cross-reactivity from true primary sensitization. Part B discusses the clinical and molecular aspects of IgE-mediated allergies to foods (including nuts, seeds, legumes, fruits, vegetables, cereal grains, milk, egg, meat, fish, and shellfish), inhalants (pollen, mold spores, mites, and animal dander), and Hymenoptera venom. Diagnostic algorithms and short case histories provide useful information for the clinical workup of allergic individuals targeted for CRD. Part C covers protein families containing ubiquitous, highly cross-reactive panallergens from plant (lipid transfer proteins, polcalcins, PR-10, profilins) and animal sources (lipocalins, parvalbumins, serum albumins, tropomyosins) and explains their diagnostic and clinical utility. Part D lists 100 important allergen molecules. In conclusion, IgE-mediated reactions and allergic diseases, including allergic rhinoconjunctivitis, asthma, food reactions, and insect sting reactions, are discussed from a novel molecular perspective. The EAACI MAUG documents the rapid progression of molecular allergology from basic research to its integration into clinical practice, a quantum leap in the management of allergic patients.

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Section: Clinical Evaluation: Diagnostic Sensitivity and Specificitymentioning

confidence: 99%

Section: Determination Of the Clinical Relevance Of An Ige Antibody Amentioning

confidence: 99%

Section: Determination Of the Clinical Relevance Of An Ige Antibody Amentioning

confidence: 99%

See 1 more Smart Citation

EAACI Molecular Allergology User's Guide

Matricardi

Kleine-Tebbe²,

Hoffmann

et al. 2016

Pediatric Allergy Immunology

Self Cite

724

870

View full text Add to dashboard Cite

show abstract

“…Therefore, only the cross-reactive antigens/ allergens shared not only with other helminths but also with mites or crustaceans should be taken into account when establishing the relationship between parasitism by ascarids and urticaria. In recent years, with the use of isolated components, the concept of cross-reactivity has been reviewed, and the concept of species-specific components and cross-reactive antigens/ allergens is becoming more accurate and much better delimited [51,71,72].…”

Section: Discussionmentioning

confidence: 99%

Urticaria and silent parasitism by Ascaridoidea: Component-resolved diagnosis reinforces the significance of this association

et al. 2020

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Urticaria remains a major problem in terms of aetiology, investigation, and management, and although parasitic diseases are considered potential causes, the absence of a consistent link between parasitic infections and skin allergy symptoms leads to the need for a deeper study of parameters that support this association. The objectives of this study were to analyse a possible relationship between parasitism by Ascarididae (Toxocara canis and Anisakis simplex) and the clinical expression of urticaria and to identify possible parasitic molecular markers for improving the diagnosis of unknown urticaria aetiology. The prevalence of Toxocara and Anisakis infestations was evaluated by measuring the levels of specific IgG (sIgG) and IgE (sIgE) antibodies against crude extracts and isolated components from whole larvae of Anisakis simplex (Ani s 1, Ani s 3 and Ani s 7) and Toxocara canis (TES-120, TES-70, TES-32 and TES-26) using immunologic and molecular diagnostic methods. A cross-sectional study was performed in a group of 400 individuals. The study group consisted of 95 patients diagnosed with urticaria (55 with chronic urticaria and 40 with acute urticaria). A control group consisted of 305 subjects without urticaria (182 diagnosed with respiratory allergy and 123 without allergy). Statistically significant differences were demonstrated in the seroprevalence of specific IgG and IgE antibodies between the urticaria patients and the healthy general population when isolated ascarid antigens were evaluated. The prevalence of IgG antibodies against Ani s 1, IgE antibodies against TES-120 and IgE antibodies against TES-70 were significantly different between the control individuals (healthy general population) and patients with urticaria. Moreover, the urticaria patient group demonstrated a higher seroprevalence of antibodies (sIgE and sIgG) against Anisakis simplex larva whole extract than the control group but just with statistically diferences when sIgE was evaluated. The presence of IgE and/or IgG antibodies against Ani s 3 (tropomyosin) can help to discriminate between patients with and without urticaria. Both ascarids seem to be associated with urticaria, although in our region, Anisakis seems to have greater involvement than Toxocara in this relationship. Molecular diagnostics can be used to associate urticaria with parasite infestations. Tropomyosin and Ani s 1 were the most relevant PLOS NEGLECTED TROPICAL DISEASES

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“…Tener un padre o hermano con rinitis, asma, DA y/o AA tiene alto riesgo de alergia. 132 Existen diferencias inmunológicas en quienes serán alérgicos desde la semana 22 de gestación. 133,134 La predisposición genética no es suficiente para explicar el aumento de la incidencia de AA.…”

Section: Prevenciónunclassified

Alergia alimentaria en pediatría: recomendaciones para su diagnóstico y tratamiento. Texto completo

Alergia¹

2018

Arch Argent Pediatr

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Molecular Allergology between Precision Medicine and the Choosing Wisely initiative

Cited by 52 publications

References 20 publications

EAACI Molecular Allergology User's Guide

EAACI Molecular Allergology User's Guide

Urticaria and silent parasitism by Ascaridoidea: Component-resolved diagnosis reinforces the significance of this association

Alergia alimentaria en pediatría: recomendaciones para su diagnóstico y tratamiento. Texto completo

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