Quantitative Measurement of Cell Surface Expression Via Magnetophoretic Cytometry

The Origins and the Current Applications of Microfluidics-Based Magnetic Cell Separation Technologies

“…In this work, immunomagnetically labeled cells were continuously deflected under an external magnetic field, and the cells in the outlets were quantified by multiplexed electrical sensors [99] as they were being sorted. Civelekoglu et al [100,101] also demonstrated high-dynamic-range operation for their magnetophoretic system, with an interesting inspiration from digital photography. By modulating the flow rate during operation, they expanded their dynamic range to cover cases with high cell heterogeneity.…”

“…By modulating the flow rate during operation, they expanded their dynamic range to cover cases with high cell heterogeneity. Later, the group presented an integrated magnetophoretic sorter (Figure 12) with computational measurements to estimate the number of magnetic beads present on the surface of the cells from their sensor reading and made a direct comparison to conventional fluorescent measurements [102,103]. Furthermore, the group showed an integrated platform that coupled their differential sorting technique with an inline magnetic positive enrichment stage profile the surface markers in blood samples directly [104], a capability that commercial flow cytometers do not possess.…”

“…They demonstrated up to 96% efficiency for their enrichment stage and an optimal processing speed of 1.5 mL/h with a throughput of up to 960 cells/s. and made a direct comparison to conventional fluorescent measurements [102,103]. Furthermore, the group showed an integrated platform that coupled their differential sorting technique with an inline magnetic positive enrichment stage profile the surface markers in blood samples directly [104], a capability that commercial flow cytometers do not possess.…”