Quantitative Measurement of 17β-Estradiol and Estriol in River Water by Time-Resolved Fluoroimmunoassay

Majima, Keisuke; Fukui, Takashi; Yuan, Jingli; Wang, Guilan; Matsumoto, Kazuko

doi:10.2116/analsci.18.869

Cited by 36 publications

(10 citation statements)

References 26 publications

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“…21 The 50 nM level of detection places the assay sensitivity needed for the concentration range commonly encountered in environmental and water effluent samples. 48,49 We examined the sensor in a more challenging fluid of spiked river water samples. Figure 3 shows that the sensor produces a similar response toward E2 spiked into river water samples as was found for double distilled water in Figure 2b.…”

Section: E2 Lateral Flow Aptasensingmentioning

confidence: 99%

Lateral Flow Aptasensor for Small Molecule Targets Exploiting Adsorption and Desorption Interactions on Gold Nanoparticles

2017

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A lateral flow assay (LFA) can provide a rapid and cost-effective means to detect targets in situ; however, existing LFA formats (predominantly sandwich assays) are not suitable for small molecule targets. We present a new LFA design that probes the dissociation of aptamers from the surface of gold nanoparticles upon recognition of small targets. The target-induced removal of aptamer molecules from the surface of the colored particles results in the particles being captured on a test line comprised of the protein bovine serum albumin immobilized on nitrocellulose. On the other hand, in the absence of target, aptamer coated particles are protected from capture on the test line and are instead captured at a control line comprised of the protein lysozyme. This protein is strongly positively charged under measurement conditions and therefore captures all gold nanoparticles regardless of the presence of aptamers. The effectiveness and operation mechanism of this simply fabricated sensor was demonstrated by using a previously reported 35-mer aptamer for a small molecule, 17β-estradiol. The sensor exhibited nanomolar level of detection, excellent selectivity against potential interfering molecules, and robust operation in natural river water samples. The simplicity and performance of the sensor platform renders it applicable to a wide range of other aptamers targeting small molecules, as we demonstrated with a novel bisphenol A aptamer. Additionally, we show that our LFA design is not confined to the specific proteins used as test and control lines, provided that their charge is appropriate to modulate the interaction with aptamer-coated or bare nanoparticles.

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Section: E2 Lateral Flow Aptasensingmentioning

confidence: 99%

Lateral Flow Aptasensor for Small Molecule Targets Exploiting Adsorption and Desorption Interactions on Gold Nanoparticles

2017

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“…Time-resolved uoroimmunoassay (TRFIA) using lanthanide chelates as uorescent labels has high sensitivity and good precision. 13,14 The most important advantage of TRFIA is the wide linear range, because the uorescence signal can be amplied million times by adding enhancement solution. Due to these benets, TRFIA is now one of the most successful quantitative immunoassays and has numerous applications in medical detection, food safety, environmental monitoring, etc.…”

Section: Introductionmentioning

confidence: 99%

A direct competitive inhibition time-resolved fluoroimmunoassay for the detection of unconjugated estriol in serum of pregnant women

Tang

Zhao

et al. 2013

Anal. Methods

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Unconjugated estriol (uE 3 ) is one of the most important serum markers for prenatal screening. The abnormally low content of uE 3 is used as an indicator of fetal DS (Down syndrome) during the second trimester in pregnant women. In the present study, we developed a time-resolved fluoroimmunoassay to detect uE 3 by employing microtiter plates with pre-captured primary antibodies. E 3 -3-CME-BSA (estriol-3-carboxymethyl ether-bovine serum albumin) conjugates served as labels and Eu 3+ (europium) as the probe for signal detection. The detection limit of this assay was 0.35 nmol L À1 . The within-run and between-run imprecision values for serum control detection were less than 5.0% and 6.0% respectively. The mean recovery was 102.6%. The long-term stability (2-8 C, 15 months) and thermostability (37 C, 10 days) were excellent. The uE 3 concentrations measured by the present assay in 1168 Chinese maternal serum samples correlated well with those obtained by the chemiluminescence immunoassay assay (r ¼ 0.948). The reference range in normal singleton pregnancies in Southern China was established which provided reference data to adjust the uE 3 medians for biochemical screening.

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“…In contrast to the GC method, HPLC chromatographic approaches enable direct separation and quantification of many compounds in aqueous samples without sample pretreatments, and has gained in popularity [24][25][26]. However, most of the reported HPLC analytical methods for estrogens focus on the synthetic estrogens such as 17a-ethynylestradiol (EE2) [27,28]. This could be due to the recently increasing contribution of anthropogenic endocrine-disrupting chemicals to environmental pollution.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of three naturally occurring estrogens in environmental waters by high‐performance liquid chromatography

Wang

Fan

et al. 2011

J of Separation Science

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A simple, sensitive and accurate reversed-phase high-performance liquid chromatographic (HPLC) method for simultaneous determination of three naturally occurring estrogenic steroids including estrone (E1), 17β-estradiol (E2) and estriol (E3) in environmental water samples was developed. Analytes were extracted with ethyl acetate solvents and preconcentrated prior to HPLC analysis. Separations were accomplished in <20 min using a reversed-phase C(18) column (4.6×250 mm id, 5 μm) with a gradient elution of mobile phase containing 3.0 mM ammonium acetate/acetonitrile mixtures (flow rate, 1.0 mL/min). UV light absorption responses at 205 nm were linear over a wide concentration range from 100,000 μg/L to the detection limits of 0.96 μg/L E1, 0.64 μg/L E2 and 0.78 μg/L E3. Quantitation was carried out by the peak area method. The relative standard deviation for the analysis of three estrogens was <3.0%. This method was applied for the simultaneous determination of estrogens in environmental water samples collected in Zhejiang, China. The higher concentrations of both E2 and E3 were found in Tang River and West Lake waters, and E1 was detected in lake water only. All three estrogens were below the detection limits in rain waters.

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Quantitative Measurement of 17β-Estradiol and Estriol in River Water by Time-Resolved Fluoroimmunoassay

Cited by 36 publications

References 26 publications

Lateral Flow Aptasensor for Small Molecule Targets Exploiting Adsorption and Desorption Interactions on Gold Nanoparticles

Lateral Flow Aptasensor for Small Molecule Targets Exploiting Adsorption and Desorption Interactions on Gold Nanoparticles

A direct competitive inhibition time-resolved fluoroimmunoassay for the detection of unconjugated estriol in serum of pregnant women

Simultaneous determination of three naturally occurring estrogens in environmental waters by high‐performance liquid chromatography

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