Quantitative liquid chromatographic–tandem mass spectrometric determination of orlistat in plasma with a quadrupole ion trap

Wieboldt, Ray; Campbell, D A; Henion, Jack D.

doi:10.1016/s0378-4347(97)00653-1

Cited by 42 publications

(34 citation statements)

References 14 publications

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“…The high degree of specificity provided by selected reaction monitoring (SRM) is a recognized strength of triple-quadrupolebased instruments, but it is also a limitation. Several investigators have described the potential advantages of using full-scan-based acquisition for quantitative analysis using a variety of mass analyzers [3][4][5][6][7][8]. High among these advantages is the ability to acquire information without the need for specific methods to be developed beforehand.…”

mentioning

confidence: 99%

Quantitative-qualitative data acquisition using a benchtop orbitrap mass spectrometer

Bateman

Kellmann

Muenster

et al. 2009

J. Am. Soc. Mass Spectrom.

178

103

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Current approaches to discovery-stage drug metabolism studies (pharmacokinetics, microsomal stability, etc.) typically use triple-quadrupole-based approaches for quantitative analysis. This necessitates the optimization of parameters such as Q1 and Q3 m/z values, collision energy, and interface voltages. These studies detect only the specified compound and information about other components, such as metabolites, is lost. The ability to perform full-scan acquisition for quantitative analysis would eliminate the need for compound optimization while enabling the detection of metabolites and other non-drug-related endogenous components. Such an instrument would have to provide sensitivity, selectivity, dynamic range, and scan speed suitable for discovery-stage quantitative studies. In this study, a prototype benchtop Orbitrap-based mass analyzer was used to collect both quantitative and qualitative data from human microsomal incubation samples as well as rat plasma from pharmacokinetic studies. Instrumental parameters such as scan speed, resolution, and mass accuracy are discussed in relation to the requirements for a quantitative-qualitative workflow. The ability to perform highly selective quantitative analysis while simultaneously characterizing metabolites from both in vitro and in vivo studies is discussed.

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mentioning

confidence: 99%

Quantitative-qualitative data acquisition using a benchtop orbitrap mass spectrometer

Bateman

Kellmann

Muenster

et al. 2009

J. Am. Soc. Mass Spectrom.

178

103

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“…The analyte/endogenous ratio is strongly sample-dependent, and affects precision and accuracy. Wieboldt, Campbell, & Henion (1998) have compared the analysis of orlistat in human plasma with a TSQ and a QIT. The assay was first developed and validated on the TSQ.…”

Section: A Mass Analyzermentioning

confidence: 99%

Quantitative high‐throughput analysis of drugs in biological matrices by mass spectrometry

Hopfgartner

Bourgogne

2003

Mass Spectrometry Reviews

254

190

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To support pharmacokinetic and drug metabolism studies, LC-MS/MS plays more and more an essential role for the quantitation of drugs and their metabolites in biological matrices. With the new challenges encountered in drug discovery and drug development, new strategies are put in place to achieve high-throughput analysis, using serial and parallel approaches. To speed-up method development and validation, generic approaches with the direct injection of biological fluids is highly desirable. Column-switching, using various packing materials for the extraction columns, is widely applied. Improvement of mass spectrometers performance, and in particular triple quadrupoles, also strongly influences sample preparation strategies, which remain a key element in the bioanalytical process.

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“…Since the late 1980s, the introduction of atmospheric pressure ionization (API) sources coupled with mass spectrometers has broadened the use of mass spectrometry (MS) in drug analysis. 10,11 They have been used to develop very specific and sensitive assays of drugs in biological samples using triple-quadrupole 12,13 or quadrupole ion traps 14 as the analyzer, and are now the method of choice as the bioanalytical tool for pharmacokinetic and metabolism studies. The use of miniaturized HPLC (i.e.…”

Section: Introductionmentioning

confidence: 99%

Fully automated determination of eserineN-oxide in human plasma using on-line solid-phase extraction with liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Pruvost

Ragueneau

Ferry³

et al. 2000

J. Mass Spectrom.

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Quantitative liquid chromatographic–tandem mass spectrometric determination of orlistat in plasma with a quadrupole ion trap

Cited by 42 publications

References 14 publications

Quantitative-qualitative data acquisition using a benchtop orbitrap mass spectrometer

Quantitative-qualitative data acquisition using a benchtop orbitrap mass spectrometer

Quantitative high‐throughput analysis of drugs in biological matrices by mass spectrometry

Fully automated determination of eserineN-oxide in human plasma using on-line solid-phase extraction with liquid chromatography coupled with electrospray ionization tandem mass spectrometry

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