“…Mice were given a subcutaneous injection of 10, 40, or 400 U/kg of Acthar (Mallinckrodt Pharmaceuticals, Bridgewater, NJ, USA), 0.6 or 1.2 mg/kg of synthetic ACTH 1–24 depot (Bachem Americas, Inc., Torrence, CA, USA), or saline (control), or a 5-mg/kg oral dose of prednisolone (Sigma-Aldrich, St. Louis, MO, USA). These doses were selected due to their established activity in rodent studies (Fiorucci et al, 2002 ; Huang et al, 2021 ; Lord et al, 2022 ). One hour later, mice received an intraperitoneal injection of 10 μg human anti-mouse anti-CD3 monoclonal antibody (HαM CD3 MAb; Clone#145-2C11; BioLegend) in saline to activate T cells.…”