Quantitative in situ fluorometry of thin-layer chromatograms

Jänchen, D.; Pataki, György

doi:10.1016/s0021-9673(00)98665-5

Cited by 43 publications

(2 citation statements)

References 10 publications

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“…The application of fluorescence quantitation to thin-layer chromatography was first reported by Seiler, Werner, and Wiechmann (25) in 1963 and described more fully in an excellent paper by R. Klaus (26) in 1964. The accuracy and precision of tic-fluorescence techniques is treated in a very good review paper by Janchen and Pataki (27).…”

Section: Instrumentationmentioning

confidence: 99%

Development of luminescence spectrometry as an analytical tool

O’Haver¹

1978

J. Chem. Educ.

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Section: Instrumentationmentioning

confidence: 99%

Development of luminescence spectrometry as an analytical tool

O’Haver¹

1978

J. Chem. Educ.

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“…Several methods allow for direct fluorescence scanning of the chromatogram. Janchen and Pataki (1968) described the Camag-Turner TLC-Scanner and demonstrated reproducibilities of 3-5% for the fluorescence method and 5 4 % for the quenching technique for results on the same chromatogram. A reproducibility of l0-15% was obtained for results compared from different chromatograms.…”

Section: A Instrumentationmentioning

confidence: 99%

Fluorescence Analysis on Solid Surfaces*

Guilbault¹

1977

Photochem & Photobiology

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Abstract-One of the recent uses of fluorescence has been in the assay of substances directly on solid surfaces such as paper chromatograms, thin-layer chromatography (TLC) plates, potassium bromide disks, electrophoresis strips, and silicone-rubber pads. Chromatography and electrophoresis are widely used analytical techniques and it is not surprising that fluorometric methods are used in conjunction with them. Advantages are increased sensitivity and rapidity of analysis.In this paper the use of fluorescence in the assay of substances after separation on paper and TLC chromatograms, as well as the direct assay of substances on a solid surface placed directly in a commercial fluorometer, are discussed. Particular emphasis is placed on the direct analysis of enzymatic reactions. ANALYSIS OF PAPER CHROMATOCRAMSDifferent grades of filter paper vary in their content of fluorescent materials, which may interfere with attempts to measure fluorescence emission from the paper. Also problems with the purity of filter paper arise; the seriousness of interference depends on the compound under analysis. Van Horst et al. (1959) 4, and S and S No. 597 were best suited for the fluorometric assay of reducing oligosaccharides on paper chromatograms.Any one of the commercially available fluorometers or fluorescence spectrometers can be used (with appropriate modification) to measure fluorescence emitted from paper chromatograms. Fluorescence can be excited by passing the exciting light through the paper, as was done by Wadman et al. (1954). Semm and Fried (1952) used a similar arrangement, although complete details were not given. The paper 403 404 GEORGE G. GUILBAULT strip can also be placed at a 45-degree angle across the cell compartment, and the fluorescence emitted from the surface can be measured.If the fluorescence from paper is measured in a spectrophotometer (reflectance) or in a fluorescence spectrometer, it should be possible to determine the entire fluorescence spectrum of a given compound. Korte and Weitkamp (1958) report procedures for measuring the fluorescence spectra of berberine, fluorescein, xanthopterin, and thiochrome on paper chromatograms. Van Horst et al. (1959) carried out a quantitative study of amino acids on paper with a Photovolt Model 525 densitometer with a phototube detector connected to a recorder. A mercury lamp was used for excitation. ANALYSIS OF TLC PLATES A. instrumentationEarly applications of fluorescence in quantitative measurements of solid surface analysis consisted principally of scraping off the substance from the solid TLC plate, or cutting the substance out of the paper chromatogram, dissolving the substance in an appropriate organic solvent, and then measuring the fluorescence in solution with a commercial fluorometer. This technique was time consuming and had limited accuracy. Hence much attention was paid to the development of instrumentation capable of direct measurement of the solid surface. As a result several attachments to commercial instruments, capable of direct measurem...

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In Vitro Metabolism of Certain Nornuciferine Derivatives

Smith

Sood

1971

Journal of Pharmaceutical Sciences

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Quantitative in situ fluorometry of thin-layer chromatograms

Cited by 43 publications

References 10 publications

Development of luminescence spectrometry as an analytical tool

Development of luminescence spectrometry as an analytical tool

Fluorescence Analysis on Solid Surfaces*

In Vitro Metabolism of Certain Nornuciferine Derivatives

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