Quantitative imaging of element spatial distribution in the brain section of a mouse model of Alzheimer's disease using synchrotron radiation X-ray fluorescence analysis

Wang, Hua Jian; Wang, Meng; Wang, Bing; Meng, Xiang Yu; Wang, Yun; Li, Ming; Feng, Weiyue; Zhao, Yu; Chai, Zhifang

doi:10.1039/b921201a

Cited by 57 publications

(44 citation statements)

References 26 publications

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“…Our study has demonstrated that normalizing the average Zn concentration in each cortical layer to that of the corpus callosum in the same brain section reduces inter- and intra-animal variability between tissue sections (see Supplementary information). Most importantly, the Zn concentration determined for each tissue layer of the cortex in this study is consistent with some previous studies (Chwiej et al, 2011; Frederickson et al, 2000; Hare et al, 2010; Lee et al, 2011; Wang et al, 2010) and falls within the normal physiological range. Likewise, the ratio of bulk Zn concentration in the cortex and corpus callosum is also in general agreement with previous findings (Chwiej et al, 2011; Sergeant et al, 2005).…”

Section: Discussionsupporting

confidence: 92%

Laminar-specific distribution of zinc: Evidence for presence of layer IV in forelimb motor cortex in the rat

et al. 2014

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The rat is the most widely studied pre-clinical model system of various neurological and neurodegenerative disorders affecting hand function. Although brain injury to the forelimb region of the motor cortex in rats mostly induces behavioral abnormalities in motor control of hand movements, behavioral deficits in the sensory-motor domain are also observed. This questions the prevailing view that cortical layer IV, a recipient of sensory information from the thalamus, is absent in rat motor cortex. Because zinc-containing neurons are generally not found in pathways that run from the thalamus, an absence of zinc (Zn) in a cortical layer would be suggestive of sensory input from the thalamus. To test this hypothesis, we used synchrotron micro X-ray fluorescence imaging to measure Zn distribution across cortical layers. Zn maps revealed a heterogeneous layered Zn distribution in primary and secondary motor cortices of the forelimb region in the adult rat. Two wider bands with elevated Zn content were separated by a narrow band having reduced Zn content, and this was evident in two rat strains. The Zn distribution pattern was comparable to that in sensorimotor cortex, which is known to contain a well demarcated layer IV. Juxtaposition of Zn maps and the images of brain stained for Nissl bodies revealed a “Zn valley” in primary motor cortex, apparently starting at the ventral border of pyramidal layer III and ending at the close vicinity of layer V. This finding indicates the presence of a conspicuous cortical layer between layers III and V, i.e. layer IV, the presence of which previously has been disputed. The results have implications for the use of rat models to investigate human brain function and neuropathology, such as after stroke. The presence of layer IV in the forelimb region of the motor cortex suggests that therapeutic interventions used in rat models of motor cortex injury should target functional abnormalities in both motor and sensory domains. The finding is also critical for future investigation of the biochemical mechanisms through which therapeutic interventions can enhance neural plasticity, particularly through Zn dependent pathways.

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Section: Discussionsupporting

confidence: 92%

Laminar-specific distribution of zinc: Evidence for presence of layer IV in forelimb motor cortex in the rat

et al. 2014

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“…The distribution of Ce and La in cucumber roots and leaves were assessed using l-XRF on BL15U beamline at Shanghai Synchrotron Radiation Facility (Shanghai, China). The experimental conditions were consistent with those of a previous report (Wang et al, 2010), except that the cross-section of the beam irradiating on the samples was about 5 Â 7 mm 2 , and the sample platform was moved at an interval of 5 lm for each step. The fluorescence intensities of Ce, La and Compton scattering were collected up to 3 s for each point.…”

Section: Elemental Mapping By Synchrotron-based Lxrfmentioning

confidence: 96%

“…The fluorescence intensities of Ce, La and Compton scattering were collected up to 3 s for each point. The fluorescence intensity was normalized to the incident X-ray intensity, and Compton scattering was used as an internal standard to compensate the differences in thickness and density of the samples (Wang et al, 2010). The corrected fluorescence intensity was used to estimate the relevant elemental content.…”

Section: Elemental Mapping By Synchrotron-based Lxrfmentioning

confidence: 99%

Origin of the different phytotoxicity and biotransformation of cerium and lanthanum oxide nanoparticles in cucumber

Zhang

et al. 2014

Nanotoxicology

131

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To investigate how the physicochemical properties of nanoparticles (NPs) affect their biological and toxicological effects, we evaluated the phytotoxicity of CeO2 and La2O3 NPs to cucumber (Cucumis sativus) plants and tried to clarify the relation between physicochemical properties of NPs and their behaviors. CeO2 NPs had no phytotoxicity to cucumber at all tested concentrations, while La2O3 NPs showed significant inhibition on root elongation (≥ 2 mg/L), shoot elongation (at 2000 mg/L), root biomass (≥ 2 mg/L), and shoot biomass (≥ 20 mg/L), as well as induced more reactive oxygen species and cell death in roots (2000 mg/L). The different distribution and speciation of Ce and La in plants were determined by synchrotron-based micro X-ray fluorescence microscopy and X-ray absorption spectroscopy. In the aerial parts, all of La was combined with phosphate or carboxylic group, while a fraction of Ce was changed to Ce(III)-carboxyl complexes, implying that La2O3 acted as its ionic form, while CeO2 displayed the behavior of particles or particle-ion mixtures. The higher dissolution of La2O3 than CeO2 NPs might be the reason for their significant difference in phytotoxicity and transporting behaviors in cucumbers. To our knowledge, this is the first detailed study of the relation between the level of dissolution of NPs and their behaviors in plant systems.

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“…For example this technique has been used to investigate the etiology and pathogenesis of disease states arising from imbalances in metal homeostasis. The links between imbalances of transition metals, such as Fe, Zn, Cu and Mn, have been investigated in brain tissue as markers for Alzheimer's disease [15][16][17] and Parkinson's disease 18,19 , in retinal tissue as a factor in Age related Macular Degeneration 20 and in liver to study Wilson's disease 21 . To differentiate regions of interest, high-spatial resolution is required on the micron scale.…”

Section: Introductionmentioning

confidence: 99%

High-Speed, Integrated Ablation Cell and Dual Concentric Injector Plasma Torch for Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

et al. 2015

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In recent years, laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) has gained increasing importance for biological analysis, where ultratrace imaging at micrometer resolution is required. However, while undoubtedly a valuable research tool, the washout times and sensitivity of current technology have restricted its routine and clinical application. Long periods between sampling points are required to maintain adequate spatial resolution. Additionally, temporal signal dispersion reduces the signal-to-noise ratio, which is a particular concern when analyzing discrete samples, such as individual particles or cells. This paper describes a novel, two-volume laser ablation cell and integrated ICP torch designed to minimize aerosol dispersion for fast, efficient sample transport. The holistic design utilizes a short, continuous diameter fused silica conduit, which extends from the point of ablation, through the ICP torch, and into the base of the plasma. This arrangement removes the requirement for a dispersive component for argon addition, and helps to keep the sample on axis with the ICP cone orifice. Hence, deposition of sample on the cones is theoretically reduced with a resulting improvement in the absolute sensitivity (counts per unit mole). The system described here achieved washouts of 1.5, 3.2, and 4.9 ms for NIST 612 glass, at full width half, 10%, and 1% maximum, respectively, with an 8–14-fold improvement in absolute sensitivity, compared to a single volume ablation cell. To illustrate the benefits of this performance, the system was applied to a contemporary bioanalytical challenge, specifically the analysis of individual biological cells, demonstrating similar improvements in performance.

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Quantitative imaging of element spatial distribution in the brain section of a mouse model of Alzheimer's disease using synchrotron radiation X-ray fluorescence analysis

Cited by 57 publications

References 26 publications

Laminar-specific distribution of zinc: Evidence for presence of layer IV in forelimb motor cortex in the rat

Laminar-specific distribution of zinc: Evidence for presence of layer IV in forelimb motor cortex in the rat

Origin of the different phytotoxicity and biotransformation of cerium and lanthanum oxide nanoparticles in cucumber

High-Speed, Integrated Ablation Cell and Dual Concentric Injector Plasma Torch for Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

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