Quantitative HPLC analysis of sunscreens and caffeine during in vitro percutaneous penetration studies

Potard, G; Laugel, Cécile; Baillet, A.; Schaefer, H.; Marty, Jean‐Paul

doi:10.1016/s0378-5173(99)00258-6

Cited by 88 publications

(52 citation statements)

References 9 publications

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“…Hot air (hair dryer: 65°C) was applied for 45 s on the human epidermis which was then immediately separated from the dermis with forceps. If the epidermis was not easily separated from the dermis, the contact time of the hot air was increased to 75 s. The dermis was cut into small pieces with a scalpel and then ground in the appropriate solvent [5] with a cutting machine, specially designed in our laboratory.…”

Section: Separation Of Epidermis From Dermismentioning

confidence: 99%

“…Isopropanol and sodium hydroxide 1 N were obtained from Fisher Scientific, acetic acid and orthophosphoric acid (H 3 PO 4 ) from Prolabo, Pic A reagent from Waters Corp., 1-heptane sulfonic acid from Sig- ma France. The complete validation of the analytical technique has recently been published [5].…”

Section: Hplc Analysesmentioning

confidence: 99%

“…These results indicate that it is not necessary to make many strippings in order to classify products according to their penetration in the horny layer. In addition, the quantification of the last tapes is difficult because of the detection limit [5].…”

Section: Distribution In the Sc According To The Strips Removedmentioning

confidence: 99%

“…This result is important, because it enabled us to reduce the exposure time of the experiment, in particular when the chemical substance (e.g. UV filter OMC) was unstable in daylight [5,11].…”

Section: Correlationmentioning

confidence: 99%

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The Stripping Technique: In vitro Absorption and Penetration of Five UV Filters on Excised Fresh Human Skin

Potard

Laugel

Schaefer

et al. 2000

Skin Pharmacol Physiol

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This article gives the results of a study whose aim was to compare the compartmental distribution and absorption of 5 UV filters, in vitro, by fresh human skin, after exposure times of 30 min and 16 h. These UV filters from BASF (octyl methoxycinnamate, benzophenone 4, benzophenone 3, octyl triazone and octocrylene) were incorporated separately in a simple oil-in-water emulsion. The composition of the emulsions was designed in order to obtain a sun protection factor of 5. Therefore the UV filters were introduced into the emulsions at different concentrations. We show that the affinity for each skin level [stratum corneum (SC), viable epidermis, dermis and receptor fluid] is different according to the test substance used. Some substances accumulated in the SC, whereas others passed through the skin very quickly and were quantified in the receptor fluid. The stripping technique allowed us to see that more than 94% of the chemical compound in the SC was in the first eight tapes. The problem of individual values below the limit of detection was raised, a correlation between the two exposure times was found (y = 1.702x – 0.105; R = 0.94) and a classification of products according to their affinity for the SC was determined.

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Section: Separation Of Epidermis From Dermismentioning

confidence: 99%

Section: Hplc Analysesmentioning

confidence: 99%

Section: Distribution In the Sc According To The Strips Removedmentioning

confidence: 99%

Section: Correlationmentioning

confidence: 99%

See 2 more Smart Citations

The Stripping Technique: In vitro Absorption and Penetration of Five UV Filters on Excised Fresh Human Skin

Potard

Laugel

Schaefer

et al. 2000

Skin Pharmacol Physiol

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“…B7 and B8: aqueous extract of tea dust at 330 nm and 275 nm respectively. method for the simultaneous determination of chlorogenic acid, caffeic acid and caffeine proved highly sensitive, the limits of detection and quantitation being lower than in similar methods published elsewhere (Pellati et al, 2005, Li et al, 2004, Potard et al, 1999 and the retention time was about 5 minutes.…”

Section: Discussionmentioning

confidence: 62%

Simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis by HPLC and correlation with antioxidant capacity of the extracts by DPPH· reduction

Rivelli

Silva

Röpke

et al. 2007

Rev. Bras. Cienc. Farm.

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A new high performance liquid chromatographic method has been established for simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts ofIlex paraguariensis. Analytical curves showed good linear regression in the concentration ranges 0.49-7.8 μg/mL for chlorogenic acid, 0.25-3.9 μg/mL for caffeic acid and 0.244-7.8 μg/mL for caffeine. Reduction of the DPPH· radical was used to determine the antioxidant capacity of the extracts. Our method for the simultaneous determination of chlorogenic acid, caffeic acid and caffeine was highly sensitive, having lower detection and quantitation limits than other papers that used similar methodology. INTRODUCTIONA free radical is any chemical species capable of independent existence that contains one or more unpaired electrons (Halliwel, Gutteridge, 1999). The role of freeradical reactions in biology has become an area of intense interest. It is generally accepted that free radicals play an important role in the development of tissue damage and pathological events in living organisms (De Groot, Noll, 1987;Schinella et al., 2000). The consumption of plant foods is associated with a reduced risk of chronic diseases (cancer and cardiovascular), in part because of substances with antioxidant capacity, and those that modulate enzyme activity and gene expression (Day et al., 2004).Antioxidative properties have been found in leaf infusions of yerba mate (Ilex paraguariensis) (Chandra, Mejia, 2004). Mate is a popular tea in South America, where it is frequently drunk instead of coffee as a stimulating drink. It is greenish in color and contains caffeine, tannins and several vitamins such as B1 (thiamine), B2 (riboflavin), B5 (pantothenic acid), C, E, β-carotene, sucrose, fructose, folic acid, trigonelline, choline and many polyphenolic compounds (flavonoids and chlorogenic acid) (Gugliucci, Stahl, 1995). Much experimental work (in vivo and in vitro) has been performed to evaluate the antioxidant capacity of yerba mate. The leaf extract of I. paraguariensis was shown to be absorbed and to be active in vivo when healthy patients drank it, and one hour later blood was collected and the LDL content analysed in relation to lipid peroxidation (Gugliucci, 1996). The Ilex paraguariensis aqueous / ascorbate system and that induced by the CCl 4 /NADPH system, in both cases detected by the thiobarbituric acid assay (Schinella et al., 2000) For the simultaneous determination of CGA (chlorogenic acid) and CFA (caffeic acid) (Figure 1) by HPLC, various sets of chromatographic conditions have been used in recent work: in Li et al. (2004), the mobile phase was a mixture of methanol and 0.2 mol/L acetate buffer (pH 3.6) (15:85, v/v) flowing at 1.0 mL/min, the detection wavelength (λ) being set at 300 nm; in Li et al. (2005), methanol:water:acetic acid (19:81:1.5, v/v) was used, flowing at 1.0 mL/min, with λ at 240 nm, and in Pellati et al. (2005), aqueous phosphoric acid solution (0.1%) and acetonitrile (90:10 v/v), flowing at 1.5 mL/min, with the...

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Lifestyle and Over‐the‐Counter Drugs

Abu‐Izza

Li²,

Parr³

2003

Burger's Medicinal Chemistry and Drug Discovery

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This chapter attempts to show how lifestyle drugs and over‐the counter (OTC) drugs relate to each other and the challenges that they face. It also traces the history of OTC drugs and regulations, shows how the OTC Review process works, and shows how the United States, Europe, and Japan classify OTC drugs. It briefly explains the three major criteria for Rx‐to‐OTC switch—safety, labeling, and effectiveness. The chapter also provides an overview and a generalized definition of lifestyle drugs. Four examples of lifestyle drugs are provided: hair growth drugs, smoking cessation drugs, sexual disorder drugs, and sunscreens. The selected examples cover both prescription only and OTC lifestyle drugs. The chapter ends with a look into the future for OTC and lifestyle drugs.

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Quantitative HPLC analysis of sunscreens and caffeine during in vitro percutaneous penetration studies

Cited by 88 publications

References 9 publications

The Stripping Technique: In vitro Absorption and Penetration of Five UV Filters on Excised Fresh Human Skin

The Stripping Technique: In vitro Absorption and Penetration of Five UV Filters on Excised Fresh Human Skin

Simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis by HPLC and correlation with antioxidant capacity of the extracts by DPPH· reduction

Lifestyle and Over‐the‐Counter Drugs

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Quantitative HPLC analysis of sunscreens and caffeine during in vitro percutaneous penetration studies

Cited by 88 publications

References 9 publications

The Stripping Technique: In vitro Absorption and Penetration of Five UV Filters on Excised Fresh Human Skin

The Stripping Technique: In vitro Absorption and Penetration of Five UV Filters on Excised Fresh Human Skin

Simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis by HPLC and correlation with antioxidant capacity of the extracts by DPPH&middot; reduction

Lifestyle and Over‐the‐Counter Drugs

Contact Info

Product

Resources

About

Simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis by HPLC and correlation with antioxidant capacity of the extracts by DPPH· reduction