Quantitative Fundus Autofluorescence in Healthy Eyes

Abstract

Normative qAF data are a reference tool essential to the interpretation of qAF measurements in ocular disease.

Clinical relevance of quantified fundus autofluorescence in diabetic macular oedema

“…Indeed, we did not find the differences of the relative FAF intensities between phakic and pseudophakic eyes with DMO (data not shown). However, we could not evaluate the absolute value of the FAF levels, compared with the methods reported by Greenberg et al 33 Despite the clinical relevance of FAF in the current study, we consider OCT to be the golden standard for diagnosing and treating DMO. 7,34 OCT provides qualitative and quantitative parameters of importance regarding DMO, although it takes longer to obtain higherquality SD-OCT images.…”

“…33 In addition, the cornea or crystalline lens might emit autofluorescent signals in patients with diabetes. 16,17 This prompted us to investigate the relative autofluorescence levels in the macular subfields compared with the signals outside the ETDRS grid, although a recent publication described a method for quantifying FAF signals using a scanning laser ophthalmoscope equipped with an internal autofluorescent reference.…”

“…16,17 This prompted us to investigate the relative autofluorescence levels in the macular subfields compared with the signals outside the ETDRS grid, although a recent publication described a method for quantifying FAF signals using a scanning laser ophthalmoscope equipped with an internal autofluorescent reference. 33 The advantage of the current method is that the FAF signal levels were adjusted by an internal control within individual images, and the relative autofluorescence levels were, to some extent, independent of the autofluorescence of the optical media. Indeed, we did not find the differences of the relative FAF intensities between phakic and pseudophakic eyes with DMO (data not shown).…”

Multimodal Imaging of Nonneovascular Age-Related Macular Degeneration

“…[26][27][28][29][30][31] This approach requires high-quality images to derive reliable quantitative measurements and may be limited by media opacities. Imaging of healthy eyes with this novel methodology has demonstrated that quantitative FAF parameters increase with age and are higher in women and may vary with ethnicity.…”

“…Imaging of healthy eyes with this novel methodology has demonstrated that quantitative FAF parameters increase with age and are higher in women and may vary with ethnicity. This modality has increasingly been used to study eyes with retinal dystrophies, such as Stargardt disease [27][28][29] and may be useful for studying areas of advanced and nascent atrophy in AMD, 31 although further efforts are needed to validate its utility for this disease.…”