Quantitative Evaluation of Bacteria Adherent and in Biofilm on Single-Wall Carbon Nanotube-Coated Surfaces

Pantanella, Fabrizio; Berlutti, Francesca; Passeri, Daniele; Sordi, Daniela; Frioni, Alessandra; Natalizi, Tiziana; Terranova, Maria Letizia; Rossi, M.; Valenti, Piera

doi:10.1155/2011/291513

Cited by 18 publications

(14 citation statements)

References 31 publications

(45 reference statements)

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“…reuteri CRL 1324. Pantanella et al (2011) demonstrated that bacterial concentrations of the inoculum affected the number of adherent bacteria of Streptococcus mutans and Ps. aeruginosa (determined after an incubation for 3 h), but did not influence the biofilm development (determined after 24 h).…”

Section: Discussionmentioning

confidence: 99%

Screening of biofilm formation by beneficial vaginal lactobacilli and influence of culture media components

et al. 2012

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Aims: To assess the ability of vaginal lactobacilli to form biofilm under different culture conditions and to determine the relationship between their growth and the capability of biofilm formation by selected strains. Methods and Results: Fifteen Lactobacillus strains from human vagina were tested for biofilm formation by crystal violet staining. Only Lactobacillus rhamnosus Centro de Referencia para Lactobacilos Culture Collection (CRL) 1332, Lact. reuteri CRL 1324 and Lact. delbrueckii CRL 1510 were able to grow and form biofilm in culture media without Tween 80. However, Lact. gasseri CRL 1263 (a non-biofilm-forming strain) did not grow in these media. Scanning electron microscopy showed that Lact. rhamnosus CRL 1332 and Lact. reuteri CRL 1324 formed a highly structured biofilm, but only Lact. reuteri CRL 1324 showed a high amount of extracellular material in medium without Tween. Conclusions: Biofilm formation was significantly influenced by the strain, culture medium, inoculum concentration, microbial growth and chemical nature of the support used for the assay. Significance and Impacts of the Study: The results allow the selection of biofilm-forming vaginal Lactobacillus strains and the conditions and factors that affect this phenomenon.

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Section: Discussionmentioning

confidence: 99%

Screening of biofilm formation by beneficial vaginal lactobacilli and influence of culture media components

et al. 2012

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“…To date, only a limited number of studies on the effect of SWCNTs on bacterial biofilm formation have been reported. For example, Rodrigues and Elimelech (2010) reported that SWCNTs showed inhibitory effects on biofilm formation by E. coli K12 cells during incubation for 48 h. Other studies used surfaces coated with SWCNTs to test the effects on biofilm formation, but controversial results were observed under different reaction conditions (Pantanella et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of single-walled carbon nanotubes on biofilm formation fromBacillus anthracisspores

Dong

Yang

2014

Biofouling

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This study reports the inhibitory effect of single walled carbon nanotubes (SWCNTs) on biofilm formation from Bacillus anthracis spores. Although the presence of 50 to 100 μg ml(-1) of SWCNTs in the suspension increased spore attachment in the wells of 96-well plates, the presence of 200 μg ml(-1) of SWCNTs in the germination solution decreased the germination percentage of the attached spores by 93.14%, completely inhibiting subsequent biofilm formation. The inhibition kinetics of 50 μg ml(-1) SWCNTs on biofilm formation showed that this concentration inhibited biofilm formation by 81.2% after incubation for 48 h. SWCNT treatment in the earlier stages of biofilm formation was more effective compared to treatment at later stages. Mature biofilms were highly resistant to SWCNT treatment.

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“…The use of in situ models to evaluate the effect of topical antimicrobial agents in oral biofilms is a reliable experimental model 23 . These models can simulate biofilms that form on the surface of teeth and are exposed to different host protective factors present in the oral cavity.…”

Section: Discussionmentioning

confidence: 99%

Oxygen tension during biofilm growth influences the efficacy antimicrobial agents

Antoniazzi

Trojahn

Casarin³

et al. 2016

Rev. odontol. UNESP

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ResumoObjetivo: Comparar a eficácia antimicrobiana de clorexidina 0,12% (CHX) e solução de chá verde (CV) (Camellia sinensis) em biofilmes formados em diferentes tensões de oxigênio em um modelo in situ. Método: Vinte e cinco estudantes de odontologia foram elegíveis. Um dispositivo com espécimes de esmalte (EE) padronizados, voltado para o palato e para vestibular foram inseridos nos voluntários por 7 dias. Durante os primeiros quatro dias nenhum agente foi aplicado. A partir do quinto dia, ambos os agentes foram aplicados nos EEs no grupo teste e nenhum agente foi aplicado no grupo EE controle. Após 7 dias, os fragmentos EEs foram removidos dos dispositivos, sonicados, plaqueados em ágar, e incubado durante 24 h a 37 °C para determinar unidades formadoras de colonias (UFCs). Resultado: CHX teve significativamente melhor eficácia em comparação com CV nos EEs para vestibular (1.330 vs. 2.170 UFC/mL) e EEs voltados para o palatal (2.250 vs. 2.520 UFC/mL). Comparações intragrupos mostraram maior eficácia em EEs vestibulares do que as EEs voltadas para o palato (1.330 vs. 2.250 UFC/mL para CHX e 2170 vs, 2520 UFC/mL para CV) em ambas as soluções. Análise dos controles, mostrou a formação de biofilme significativamente maior em comparação com EEs palatinas e bucais. Conclusão: CHX tem uma eficácia maior do que o chá verde em biofilmes formados por 4 dias. A eficácia dos agentes foi reduzida para os biofilmes em ambiente com baixa tensão de oxigênio. A tensão de oxigênio parece influenciar na eficácia dos agentes testados.Descritores: Camellia sinensis; clorexidina; placa dentária. AbstractObjective: To compare the antimicrobial efficacy of a 0.12% chlorhexidine (CHX) and herbal green tea (Camellia sinensis) solution on established biofilms formed at different oxygen tensions in an in situ model. Method: Twenty-five dental students were eligible for the study. In situ devices with standardized enamel specimens (ES) facing the palatal and buccal sides were inserted in the mouths of volunteers for a 7 day period. No agent was applied during the first four days. From the fifth day onward, both agents were applied to the test ES group and no agent was applied to the control ES group. After 7 days the ES fragments were removed from the devices, sonicated, plated on agar, and incubated for 24 h at 37 °C to determine and quantify the colony forming units (CFUs). Result: CHX had significantly higher efficacy compared to green tea on the buccal (1330 vs. 2170 CFU/µL) and palatal (2250 vs. 2520 CFU/µL) ES. In addition, intragroup comparisons showed significantly higher efficacy in buccal ES over palatal ES (1330 vs. 2250 CFU/µL for CHX and 2170 vs, 2520 CFU/µL for CV) for both solutions. Analysis of the ES controls showed significantly higher biofilm formation in palatal ES compared to buccal ES. Conclusion: CHX has higher efficacy than green tea on 4-day biofilms. The efficacy of both agents was reduced for biofilms grown in a low oxygen tension environment. Therefore, the oxygen tension environment seems to influence the eff...

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Quantitative Evaluation of Bacteria Adherent and in Biofilm on Single-Wall Carbon Nanotube-Coated Surfaces

Cited by 18 publications

References 31 publications

Screening of biofilm formation by beneficial vaginal lactobacilli and influence of culture media components

Screening of biofilm formation by beneficial vaginal lactobacilli and influence of culture media components

Inhibitory effects of single-walled carbon nanotubes on biofilm formation fromBacillus anthracisspores

Oxygen tension during biofilm growth influences the efficacy antimicrobial agents

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