Quantitative Estimation of Lactylated Glycerides and Polyglycerol Esters in Shortenings Containing Mono-and Diglycerides

Abstract

A column chromatographic method has been developed for the quantitative estimation of lactylated glycerides and polyglycerol esters in shortenings. The components are eluted successively with benzene, benzene with 10% ethyl ether, ethyl ether, and ethyl alcohol. Lactylated glycerides eluted along with the corresponding mono- and diglycerides are estimated as lacto-palmitates from their lactic acid content. Polyglycerol esters are quantitatively eluted with ethyl alcohol. Results of analyses of several combinations of emulsifiers in lard and a vegetable shortening yielded recoveries of 94 to 102%.

Lipid composition of oats (Avena sativa L.)

“…A one g sample of the purified lipid was fractionated on a 29 g silicic acid column described by Sahasrubudhe et al (13). The various lipid classes were eluted in 10.5 ml fractions in 170 x 25 ml tubes on a LKB fraction collector by successive development with solvents of increasing polarity.…”

Derivative Formation in the Chromatographic Analysis of Food Additives

“…This allowed the emulsifier to be analyzed intact. Sahasrabudhe's (and co-workers) (17) work in this area deserves special mention. Employing an initial fractionation into lipid classes by silicic acid column chromatography, Sahasrabudhe and co-workers developed GLC procedures for the characterization and analysis of the TMS derivatives of mono-and diglycerides (18), polyglycerol fatty acid esters (19), propylene glycol fatty acid esters (20), and the sorbitan fatty acid esters (21).…”

Gas‐liquid chromatographic analysis of mono‐and diglycerides

“…fl-Monoglycerides were prepared from simple triglycerides by selective splitting with pancreatic lipase, followed with the separation of the lipid classes on a silieic acid column (12). In some instances, where more than one g of material was available, the chromatographic procedure of Quinlin and Weiser (13), as described by these authors (14), was employed.…”

“…Commercial mono-and diglycerides and shortening compositions prepared in the laboratory, containing various proportions of mono-and diglycerides, were analyzed as follows. The sample was fractionated into lipid classes by silicie acid column chromatography as described earlier (14), and the percentage distribution of total mono-and diglycerides was calculated from actual weights in each fraction. Then fractions containing monoglycerides and diglycerides were converted to TMS derivatives and analyzed by GLC for individual ester distribution.…”