Quantitative enantiomeric analysis of chlorcyclizine, hydroxyzine, and meclizine by capillary electrophoresis

Abstract

A simple capillary zone electrophoresis method was developed for the quantitative enantiomeric analysis of piperazine antihistamines with teratogenic suspicion in animals. Enantioseparation of chlorcyclizine, hydroxyzine, and meclizine was performed in glycine buffer (0.6 mol L(-1); pH 3.00) with sulfated beta-cyclodextrin (5 mg mL(-1)) as a chiral selector; and the separated drugs were monitored by ultra-violet detector. The lower quantitation of the individual enantiomer is attainable at 10 micro mol L(-1), using an achiral piperazine drug (cyclizine) as internal standard. The method is simple and rapid with a short run time (<5 min) for the analysis of chlorcyclizine, hydroxyzine or meclizine enantiomers.

Sensitive Detection of Haloperidol and Hydroxyzine at Multi-Walled Carbon Nanotubes-Modified Glassy Carbon Electrodes

“…Based on their importance, some analytical methods have been applied to investigate their characteristics, such as spectrophotometry [3], high-performance liquid chromatography [4, 5], capillary electrophoresis [6], mass spectrometry, etc. [7].…”

Quantification of meclizine in human plasma by high performance liquid chromatography–mass spectrometry

“…A gas chromatography-mass spectrometry method was developed in 1977, but it was not well validated and the sensitivity was low and not suitable for the clinical pharmacokinetic study [5]. Other assay methods, such as HPLC-UV and capillary zone electrophoresis, were also developed and validated to detect meclizine in pharmaceutical preparation as well as in bio-samples, but the sensitivity is too low with LLOQ more than 500 ng/ml [6][7][8]. To facilitate clinical pharmacokinetic study of meclizine, a sensitive and specific high performance liquid chromatography-mass spectrometric method was developed and validated in terms of the accuracy, precision, reproducibility, and recovery as well.…”

Chiral separation and quantitation of cetirizine and hydroxyzine by maltodextrin‐mediated CE in human plasma: Effect of zwitterionic property of cetirizine on enantioseparation

“…A literature survey reveals that some methods have been reported for the enantioseparation of HZ by affinity electrokinetic chromatography (AEKC) 6, NMR spectroscopy 7, CEC 8, normal and reversed‐phase nano‐HPLC 9 and CE 10, 11. In some of these methods, human serum albumin 6, and native 7 and anionic CDs 10, 11 were used as chiral selectors.…”

“…A literature survey reveals that some methods have been reported for the enantioseparation of HZ by affinity electrokinetic chromatography (AEKC) 6, NMR spectroscopy 7, CEC 8, normal and reversed‐phase nano‐HPLC 9 and CE 10, 11. In some of these methods, human serum albumin 6, and native 7 and anionic CDs 10, 11 were used as chiral selectors. Also, monolithic silica capillary column modified by coating of cellulose tris(3,5‐dimethylphenylcarbamate) 8 and fused‐silica capillaries packed with silica gel, which was modified by covalent attachment of poly‐ N ‐acryloyl‐l‐phenylalanine ethyl ester 9 as chiral columns, were used for enantioseparation of HZ by CE or nano‐HPLC.…”