Chiral separation and quantitation of cetirizine and hydroxyzine by maltodextrin‐mediated CE in human plasma: Effect of zwitterionic property of cetirizine on enantioseparation

Abstract

In the present study, a very simple CE method for chiral separation and quantitation of zwitterionic cetirizine (CTZ), as the main metabolite of hydroxyzine (HZ), and HZ has been developed. In addition, the effect of zwitterionic property of CTZ on enantioseparation was investigated. Maltodextrin, a linear polysaccharide, as a chiral selector was used and several parameters affecting the separation such as pH of BGE, concentration of chiral selector and applied voltage were studied. The best BGE conditions for CTZ and HZ enantiomers were optimized as 75 mM sodium phosphate solution at pH of 2.0, containing 5% w/v maltodextrin. Results showed that, compared to HZ, pH of BGE was an effective parameter in enantioseparation of CTZ due to the zwitterionic property of CTZ. The linear range of the method was over 30-1200 ng/mL for all enantiomers of CTZ and HZ. The quantification and detection limits (S/N=3) of all enantiomers were 30 and 10 ng/mL, respectively. The method was used to quantitative enantioseparation of CTZ and HZ in spiked human plasma.

A Dispersive Liquid–Liquid Microextraction and Chiral Separation of Carvedilol in Human Plasma Using Capillary Electrophoresis

“…Electroosmotic flow (EOF), Joule heating and the adsorption of analyte on the inner surface of capillary column, are the phenomena which were influenced by buffer concentration [35]. The 0.6% (w/v) CM-β-CD and 30% (v/v) methanol at pH 2.5 BGE were applied for the optimization of phosphoric acid-TEA buffer (30-100 mM) concentration.…”

“…Temperature variations affect enantioseparation with different mechanisms, for example, buffer viscosity, Joule heating, migration times and complex formation equilibria between enantiomers and chiral selector [35,36]. Several capillary column temperatures (15, 20 and 25°C) were checked.…”

New approaches in sensitive chiral CE

“…Thus, this sample treatment continues being one of the most employed. Table shows the use of LLE for the enantiomeric determination of drugs in biological samples as plasma or microsomal fraction of liver homogenates , and of fungicides in environmental samples as slurries . After the sample treatment, LODs from 7 × 10 −8 to 5 × 10 −6 M were reported when UV detection was used.…”

Recent advances in electrodriven enantioseparations

“…Several methods for the assay of β‐blockers , trimipramine , warfarin , cetirizine and hydroxyzine , L ‐methotrexate and cimaterol, clenbuterol, and terbutaline in spiked biological matrices were developed. Details of the experimental settings are listed in Table .…”