Papaver bracteatum was established as cell suspension culture, as suspension culture with callus grown roots, and as suspension culture containing embryoids and shoots. Alkaloid analysis using thin layer chromatography and reverse phase high pressure liquid chromatography detected the presence of thebaine (0.007%) in shoot cultures. Papaver somniferum cell suspension cultures and suspension cultures containing callus grown roots were established also. Cryptopine was isolated and identified by its mass spectrum.