Alkaloid Production From Papaver Tissue Cultures

Staba, E. John; Zito, S. William; Amin, Muhammad Nurul

doi:10.1021/np50021a004

Cited by 45 publications

(17 citation statements)

References 10 publications

(12 reference statements)

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“…This discrepancy has several different explanations: the use of different analytical methods with varied sensibilities, the use of different Papaver somniferum cultivars [7] and the analysis of somatic tissue cultures at various stages of differentiation. It is known that organogenesis or embryogenesis are accompanied by morphinan alkaloid accumulation [24,25]. We have also established Papaver somniferum tissue cultures showing different degrees of differentiation controlled by various hormonal conditions.…”

Section: Correlation Between Organogenesis Somatic Embryogenesis Andmentioning

confidence: 99%

Production of Alkaloids in Plant Cell and Tissue Cultures

Laurain-Mattar¹

2008

Bioactive Molecules and Medicinal Plants

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A low or no productivity of alkaloids in plant cell cultures can be explained by an insufficient level of cell differentiation. The first strategy described in this chapter for improving isoquinoline alkaloid accumulation is organogenesis and somatic embryogenesis induced by the addition of exogenous growth regulators in Papaver somniferum and Leucojum aestivum cell cultures. The second strategy described is the transformation of medicinal plants (Atropa belladonna, Papaver somniferum, and Leucojum aestivum) using Agrobacterium rhizogenes to form hairy root cultures, which carry with them with the benefits of fast growth and rates of alkaloid production equal to or greater than that found for the intact plant.

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Section: Correlation Between Organogenesis Somatic Embryogenesis Andmentioning

confidence: 99%

Production of Alkaloids in Plant Cell and Tissue Cultures

Laurain-Mattar¹

2008

Bioactive Molecules and Medicinal Plants

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“…Although other HPLC methods for the separation of one or several alkaloids of Pupauer spp. have been reported (Hodges and Rapoport, 1982;Kamo et a/., 1982;Staba et al, 1982;Khanna and Shukla, 1986;Roberts ef al., 1987;Tyler et al, 1988), these have often involved buffered mobile phases, two solvent systems and band asymmetry. To our knowledge, a rapid and simple HPLC method for the qualitative and quantitative evaluation of several major alkaloids of P. sorntziferurn and the alkaloid precursor L-tyrosine has not been previously described.…”

Section: Resultsmentioning

confidence: 90%

A rapid high performance liquid chromatographic method for the separation of the alkaloid precursor L‐tyrosine and six tetrahydroisoquinoline alkaloids of Papaver somniferum

Kraml

DiCosmo

1993

Phytochemical Analysis

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A high performance liquid chromatographic (HPLC) method was developed for the qualitative and quantitative determination of L-tyrosine and six common tetrahydroisoquinoline alkaloids (papaverine, noscapine, sanguinarine, morphine, codeine and thebaine) of Pupuuer somniferum. The reversed phase HPLC method yields baseline separation of the alkaloids in 20 min and is achieved using a simple H,O: MeOH linear gradient. Silanol effects commonly associated with the separation of such strongly basic compounds were minimized by the addition of the amine modifier, triethylamine, to the mobile phase.

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“…61 MS/MS spectra from both the TCD-IMS/MS system and Orbitrap identify 370 m/z as cryptopine (C 21 H 23 NO 5 ), as described before. 62 The relationship between opium poppy and the alkaloids N,N-dimethylnarcotine, reticuline, and cryptopine has been previously established and is not the result of chemical interferences in the seized samples. 63 The peak at 268 m/z occurs as a loss of water from morphine, 55 and water loss from codeine accounts for the ion at 282 m/z.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Unambiguous Characterization of Analytical Markers in Complex, Seized Opiate Samples Using an Enhanced Ion Mobility Trace Detector-Mass Spectrometer

et al. 2014

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Ion mobility spectroscopy (IMS)-based trace-compound detectors (TCDs) are powerful and widely implemented tools for the detection of illicit substances. They combine high sensitivity, reproducibility, rapid analysis time, and resistance to dirt with an acceptable false alarm rate. The analytical specificity of TCD-IMS instruments for a given analyte depends strongly on a detailed knowledge of the ion chemistry involved, as well as the ability to translate this knowledge into field-robust analytical methods. In this work, we introduce an enhanced hybrid TCD-IMS/mass spectrometer (TCD-IMS/MS) that combines the strengths of ion-mobility-based target compound detection with unambiguous identification by tandem MS. Building on earlier efforts along these lines (Kozole et al., Anal. Chem. 2011, 83, 8596-8603), the current instrument is capable of positive and negative-mode analyses with tightly controlled gating between the IMS and MS modules and direct measurement of ion mobility profiles. We demonstrate the unique capabilities of this instrument using four samples of opium seized by the Canada Border Services Agency (CBSA), consisting of a mixture of opioid alkaloids and other naturally occurring compounds typically found in these samples. Although many analytical methods have been developed for analyzing naturally occurring opiates, this is the first detailed ion mobility study on seized opium samples. This work demonstrates all available analytical modes for the new IMS-MS system including "single-gate", "dual-gate", MS/MS, and precursor ion scan methods. Using a combination of these modes, we unambiguously identify all signals in the IMS spectra, including previously uncharacterized minor peaks arising from compounds that are common in raw opium.

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Alkaloid Production From Papaver Tissue Cultures

Cited by 45 publications

References 10 publications

Production of Alkaloids in Plant Cell and Tissue Cultures

Production of Alkaloids in Plant Cell and Tissue Cultures

A rapid high performance liquid chromatographic method for the separation of the alkaloid precursor L‐tyrosine and six tetrahydroisoquinoline alkaloids of Papaver somniferum

Unambiguous Characterization of Analytical Markers in Complex, Seized Opiate Samples Using an Enhanced Ion Mobility Trace Detector-Mass Spectrometer

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