Quantitative determination of sulpyrid in biological samples from rats by gas-liquid chromatography and chemical ionization-mass fragmentography

Liquid chromatography–tandem mass spectrometry quantification of levosulpiride in human plasma and its application to bioequivalence study

“…This procedure proved much simpler than previous LC-MS [20,21] and chromatographic methods [7][8][9][10][11][12][13][14][15]. The amount of plasma used (100 L) was significantly lower than that in previous studies [5][6][7][8][9][10][11][12][13][14][15][16]21].…”

“…High-performance liquid chromatography (HPLC) is a well-established technique, but is limited by poor specificity and long analysis times. Gas chromatographic methods suffer from laborious sample derivatization procedures [15,16]. Improvements in sensitivity, analysis time, and specificity have been achieved using liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods [17][18][19].…”

Determination of Sulpiride in Human Urine Using Excitation-Emission Matrix Fluorescence Coupled with Second-order Calibration

“…A series of highly sensitive analytical methods have been developed for the determination of sulpiride, including gas chromatography (GC) with mass spectrometric 5,6 or oscillopolarographic detection, 7 high-performance liquid chromatography (HPLC) with ultraviolet, [8][9][10] fluorescence 11 or mass spectrometric detection, 12 capillary electrophoresis with electrochemiluminescence (CE-ECL), 2 cyclodextrin additives 3 or ultraviolet. 13 Unfortunately, all of these measurement techniques are time-consuming and may be complicated by the need for tedious pretreatment procedures, i.e., sample extraction in the routinely used HPLC methods [8][9][10][11][12] or CE-ECL analysis.…”

Improvement and validation of a liquid chromatographic method for the determination of levosulpiride in human serum and urine

“…The earlier methods of analysis for levosulpiride were based on spectrofluorometric method [6], gas chromatography (GC) [7,8], high-performance liquid chromatography (HPLC) with UV detector [4,9,10] and fluorescence detector [11][12][13][14]. Most of these methods involve tedious [7,8], time-consuming extraction procedures using a relatively large sample volume [9,11] and do not provide good assay sensitivity for pharmacokinetic studies of levosulpiride [6][7][8][9][10][11][12][13][14] at the single oral dose of 25 mg. A simple, rapid, sensitive and reliable method for the determination of levosulpiride in human serum and urine was required for further investigations of its pharmacokinetics.…”

“…Most of these methods involve tedious [7,8], time-consuming extraction procedures using a relatively large sample volume [9,11] and do not provide good assay sensitivity for pharmacokinetic studies of levosulpiride [6][7][8][9][10][11][12][13][14] at the single oral dose of 25 mg. A simple, rapid, sensitive and reliable method for the determination of levosulpiride in human serum and urine was required for further investigations of its pharmacokinetics.…”