QUANTITATIVE DETERMINATION OF STARCH AND GLYCOGEN AND THEIR METABOLISM IN LEAVES OF <i>TUSSILAGO FARFARA</i> DURING INFECTION BY <i>PUCCINI A POARUM</i>

Holligan, P. M.; McGEE, E. E. M.

doi:10.1111/j.1469-8137.1974.tb01316.x

Cited by 19 publications

(11 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Starch was assayed by grinding the residue after ethanol extraction with amyloglucosidase and measuring glucose released with glucose oxidase by methods essentially similar to those of HoUigan, McGee and Lewis (1974).…”

Section: Experimental Methodsmentioning

confidence: 99%

Stimulation of Photosynthetic Starch Formation by Sequestration of Cytoplasmic Orthophosphate

1975

Self Cite

View full text Add to dashboard Cite

SUMMARYPhotosynthetic starch formation in leaf discs of spinach beet and related species can be increased more than ten fold by the presence of exogenous mannose at io~^ M. In these species, carbon from mannose is not incorporated into starch to a significant extent and the stimulation is believed to be an indirect effect resulting from the sequestration of cytoplasmic orthophosphate as mannose phosphate. In species such as tobacco, there is substantial incorporation of carbon from mannose into starch and into soluble compounds other than mannose-6-phosphate.

show abstract

Section: Experimental Methodsmentioning

confidence: 99%

Stimulation of Photosynthetic Starch Formation by Sequestration of Cytoplasmic Orthophosphate

1975

Self Cite

View full text Add to dashboard Cite

show abstract

“…Aspects of the carbohydrate metabolism of this association have already been reported (Holligan, Chen and Lewis, 1973;HoUigan e< a/., 1974a;Holligan, McGee and Lewis, 1974b).…”

Section: Introductionmentioning

confidence: 65%

LIPID METABOLISM IN LEAVES OF TUSSILAGO FARFARA DURING INFECTION BY PUCCINIA POARUM

Lösel¹,

Lewis²

1974

New Phytologist

Self Cite

View full text Add to dashboard Cite

SUMMARYFollowing infection by Puccinia poarum, there is an accumulation of lipid in leaf tissue of Tussilago farfara. Most of this increase is in the fungus, which accounts for nearly half of the dry weight of mature aecial pustules. While the lipid content of infected tissue rises to more than three times that of healthy leaves, that of the uninfected parts of diseased leaves is reduced by one-third. Aecial pustules accumulate a high proportion of neutral lipid, particularly triglycerides and fatty acids. As infection progresses, a loss of chloroplast glycolipids and phosphatidyl glycerol is accompanied hy increases in phosphatidyl ethanolamine and phosphatidyl choline and the formation of phosphatidyl serine, which is not readily detectable in healthy leaf tissue. The lipid changes following rust infection have been compared with those of senescing Tussilago leaves.Both neutral lipids and phospholipids incorporated more photosynthetically assimilated '*C in rust-infected tissue than in healthy leaves. Greatest differences were in free fatty acids, triglycerides, phosphatidyl ethanolamine and phosphatidyl choline with significant increases also in sterols, phosphatidyl inositol and phosphatidyl serine. Whereas three-quarters of the activity incorporated from ''*CO2 into lipid appears in the neutral lipids of diseased tissue, in healthy tissue these contain only one-quarter.

show abstract

“…Instead, sucrose and starch, which is labile in the dark but stable in the light, become most heavily labelled. This starch, present at low levels compared with the fructans and extractable from leaves by oxalic acid and amyloglucosidase, is characterized and discussed further by Holligan et al (1974)The retention of radioactivity in starch in the light is shown by both autoradiography and chemical analysis (Plate i and Table 2) and appears to account for the smaller amount of radioactivity which moves from host to parasite in the light. Under these conditions, unlabelled sugars from continuing photosynthesis are available to the fungus.…”

Section: Discussionmentioning

confidence: 99%

“…Recently synthesized photosynthate, particularly in the dark when starch is utilized, passes rapidly to the fungus where it is transformed to polyols (Table 3), lipids (Table 2), a glucomannan and glycogen (see Holligan et al, 1974), together with the products of intermediary metabolism and insoluble compounds not analysed by the methods used (see Plate 2, No. 8c).…”

Section: Discussionmentioning

confidence: 99%

“…All samples were normally extracted in methanol-chloroform-water (12:5:3 v/v) or aqueous ethanol followed by water and 1.5 N H2SO4 as described by Holligan et al (1973). However, in one experiment, instead of the aqueous extraction, the tissue was homogenized and treated with amyloglucosidase (Sigma type HI, 0.5 mg/ml in water) at 45° C for 2 h. This procedure extracts a-glucans, which are hydrolysed to glucose, and water-soluble polysaccharides (see Holligan, McGee and Lewis (1974) for details). In another experiment, intact discs, after extraction in aqueous ethanol were further extracted with 0.1 N oxalic acid for i h before treatment with 1.5 N H2SO4.…”

Section: Treatment Of Leaf Tissue After Feeding With âmentioning

confidence: 99%

See 1 more Smart Citation

Carbohydrate Metabolism in Healthy and Rusted Leaves of Coltsfoot

1974

Self Cite

View full text Add to dashboard Cite

Summary Infection of leaves of Tussilago by Puccinia poarum results in decreased export of photosynthetic products and, when infection is severe, in increased import. Photosynthetic products move to infection sites where host metabolites are converted to specific fungal products, including mannitol, arabitol, trehalose, glycogen, glucomannan and lipids. More previously fixed 14C is translocated to the fungus in the dark than in the light since, in the dark, degradation of starch contributes to net movement of 14C.

show abstract

QUANTITATIVE DETERMINATION OF STARCH AND GLYCOGEN AND THEIR METABOLISM IN LEAVES OF TUSSILAGO FARFARA DURING INFECTION BY PUCCINI A POARUM

Cited by 19 publications

References 10 publications

Stimulation of Photosynthetic Starch Formation by Sequestration of Cytoplasmic Orthophosphate

Stimulation of Photosynthetic Starch Formation by Sequestration of Cytoplasmic Orthophosphate

LIPID METABOLISM IN LEAVES OF TUSSILAGO FARFARA DURING INFECTION BY PUCCINIA POARUM

Carbohydrate Metabolism in Healthy and Rusted Leaves of Coltsfoot

Contact Info

Product

Resources

About