Quantitative Determination of Proteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Nelson, Randall W.; McLean, Matthew A.; Hutchens, T. William

doi:10.1021/ac00081a008

Cited by 130 publications

(107 citation statements)

References 14 publications

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“…The main characteristics and applications of MALDI TOF-MS have been presented in some review articles. [27][28][29][30][31][32][33][34][35] Recent works dealing with the use of the technique to the identification and structural analysis of various compounds can be mentioned. [36][37][38][39][40][41][42][43][44][45][46][47][48] Coupling of MALDI with liquid chromatography, capillary electrophoresis (CE) and gel chromatography is a way to extend its applicability.…”

Section: An Overview Of Analytical Applications Of Tof-ms Analyzersmentioning

confidence: 99%

An Overview of Analytical Applications of Time of Flight-Mass Spectrometric (TOF-MS) Analyzers and an Inductively Coupled Plasma-TOF-MS Technique

Balcerzak

2003

ANAL. SCI.

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Section: An Overview Of Analytical Applications Of Tof-ms Analyzersmentioning

confidence: 99%

An Overview of Analytical Applications of Time of Flight-Mass Spectrometric (TOF-MS) Analyzers and an Inductively Coupled Plasma-TOF-MS Technique

Balcerzak

2003

ANAL. SCI.

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“…It relies on co-crystallizations of trace analytes with matrix compounds, but the typical sample morphology and spatial distribution of analytes are highly irregular. The heterogeneous analyte distributions result in poor data reproducibility [2,6,7] and make quantification analysis with MALDI mass spectrometry (MS) difficult [8]. The reasons for a heterogeneous spatial distribution of molecules, or heterogeneity defined hereafter, may involve multiple factors during droplet drying processes, such as physical properties of solutes [9], viscosity of solutions [10], and hydrodynamic flows inside droplets [11].…”

Section: Introductionmentioning

confidence: 99%

Reducing Spatial Heterogeneity of MALDI Samples with Marangoni Flows During Sample Preparation

Lai

Cai

Lee

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. This work demonstrates a method to prepare homogeneous distributions of analytes to improve data reproducibility in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). Natural-air drying processes normally result in unwanted heterogeneous spatial distributions of analytes in MALDI crystals and make quantitative analysis difficult. This study demonstrates that inducing Marangoni flows within drying droplets can significantly reduce the heterogeneity problem. The Marangoni flows are accelerated by changing substrate temperatures to create temperature gradients across droplets. Such hydrodynamic flows are analyzed semi-empirically. Using imaging mass spectrometry, changes of heterogeneity of molecules with the change of substrate temperature during drying processes are demonstrated. The observed heterogeneities of the biomolecules reduce as predicted Marangoni velocities increase. In comparison to conventional methods, drying droplets on a 5°C substrate while keeping the surroundings at ambient conditions typically reduces the heterogeneity of biomolecular ions by 65%-80%. The observation suggests that decreasing substrate temperature during droplet drying processes is a simple and effective means to reduce analyte heterogeneity for quantitative applications.

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“…In the work of Nelson et al [1], where MALDI-TOF was used for protein quantification, their results demonstrated a linear relationship of molecular ion signals to the concentration of protein in solution. This method used the normalization of analyte molecular ion signals to the signals of internal reference molecular ions.…”

mentioning

confidence: 98%

“…The principal activity in this area has been reported by Nelson et al [1] as well as by Hercules et al [2][3][4][5][6]. In their studies, proteins and peptides were analyzed and the intensities of single peaks were considered.…”

mentioning

confidence: 99%

Quantitative analysis of technical polymer mixtures by matrix assisted laser desorption/ionization time of flight mass spectrometry

Yan¹,

Gardella

Wood

2002

J. Am. Soc. Mass Spectrom.

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We report quantitative MALDI-TOF measurements for polydimethylsiloxane (PDMS) of two different molecular weights using the relative ratio of the signal intensities of integrated oligomer distributions for these two molecular weight distributions. By reporting the ratio of intensities of the integrals of two oligomer distributions, we assume that the ionization and desorption efficiencies, crystallization conditions and other factors affecting intensity are similar. Poly(methyl methacrylate) (PMMA-33,000) was mixed with PDMS samples to show whether the presence of another material might affect the desorption efficiency. Quantitative values for the number-average molecular weight (M n ), weight-average molecular weight (M w ) and polydispersities (D) were calculated using the oligomer distributions. The results show a linear relationship between the analyte concentrations and the signal intensities in the range from 1,000 Da to 10,000 Da, and the desorption efficiency of these two PDMS materials was the same even in the presence of PMMA. (J Am Soc Mass Spectrom 2002, 13, 914 -920)

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Quantitative Determination of Proteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Cited by 130 publications

References 14 publications

An Overview of Analytical Applications of Time of Flight-Mass Spectrometric (TOF-MS) Analyzers and an Inductively Coupled Plasma-TOF-MS Technique

An Overview of Analytical Applications of Time of Flight-Mass Spectrometric (TOF-MS) Analyzers and an Inductively Coupled Plasma-TOF-MS Technique

Reducing Spatial Heterogeneity of MALDI Samples with Marangoni Flows During Sample Preparation

Quantitative analysis of technical polymer mixtures by matrix assisted laser desorption/ionization time of flight mass spectrometry

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