Reducing Spatial Heterogeneity of MALDI Samples with Marangoni Flows During Sample Preparation

Lai, Yin‐Hung; Cai, Yi-Hong; Lee, Hsun; Ou, Yumeng; Hsiao, Chih-Hao; Tsao, Chien-Wei; Wang, Yi-Sheng

doi:10.1007/s13361-016-1406-0

Cited by 32 publications

(25 citation statements)

References 43 publications

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“…Understanding and controlling the process of solute deposition in the presence of coffee-ring effect (CRE) is important in manufacturing processes involving evaporation on surfaces including printing [6][7][8][9] and fabrication of ordered structures [24], functional nanomaterials [25,26] and colloidal crystals [27,28]. CRE also hampers the performance of commercial applications including fluorescent microarrays [10,11], matrix assisted laser desorption ionization (MALDI) spectrometry [12][13][14][15], and surface enhanced Raman spectroscopy (SERS) [16,17]. CRE has also implications in plasmonics [18], diagnostics [19][20][21], solute separation [22] and electronics applications [23].…”

Section: Introductionmentioning

confidence: 99%

A review on suppression and utilization of the coffee-ring effect

Mampallil

Eral

2018

Advances in Colloid and Interface Science

494

388

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Evaporation of sessile droplets containing non-volatile solutes dispersed in a volatile solvent leaves behind ring-like solid stains. As the volatile species evaporates, pinning of the contact line gives rise to capillary flows that transport non-volatile solutes to the contact line. This phenomenon, called the coffee-ring effect, compromises the overall performance of industrially relevant manufacturing processes involving evaporation such as printing, biochemical analysis, manufacturing of nano-structured materials through colloidal and macromolecular patterning. Various approaches have been developed to suppress this phenomenon, which is otherwise difficult to avoid. The coffee-ring effect has also been leveraged to prepare new materials through convection induced assembly. This review underlines not only the strategies developed to suppress the coffee-ring effect but also sheds light on approaches to arrive at novel processes and materials. Working principles and applicability of these strategies are discussed together with a critical comparison.

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Section: Introductionmentioning

confidence: 99%

A review on suppression and utilization of the coffee-ring effect

Mampallil

Eral

2018

Advances in Colloid and Interface Science

494

388

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“…For example, improved α-cyclodextrin (α-CD) signal homogeneity with THAP and α-cyano-4-hydroxycinnamic acid (CHCA) as the matrix under lowtemperature sample drying conditions has been reported recently. 14 The disadvantage with changing sample plate temperature is that the method is currently unsuitable for high-throughput analysis due to the long sample drying time in low-temperature conditions.…”

Section: Discussionmentioning

confidence: 99%

Preparation of Homogeneous MALDI Samples for Quantitative Applications

Tsao

Lai

et al. 2016

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This protocol demonstrates a simple sample preparation to reduce spatial heterogeneity in ion signals during matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The heterogeneity of ion signals is a severe problem in MALDI, which results in poor data reproducibility and makes MALDI unsuitable for quantitative analysis. By regulating sample plate temperature during sample preparation, thermal-induced hydrodynamic flows inside droplets of sample solution are able to reduce the heterogeneity problem. A room-temperature sample preparation chamber equipped with a temperature-regulated copper base block that holds MALDI sample plates facilitates precise control of the sample drying condition. After drying of sample droplets, the temperature of sample plates is returned to room temperature and removed from the chamber for subsequent mass spectrometric analysis. The areas of samples are examined with MALDI-imaging mass spectrometry to obtain the spatial distribution of all components in the sample. In comparison with the conventional dried-droplet method that prepares samples under ambient conditions without temperature control, the samples prepared with the method demonstrated herein show significantly better spatial distribution and signal intensity. According to observations using carbohydrate and peptide samples, decreasing substrate temperature while maintaining the surroundings at ambient temperature during the drying process can effectively reduce the heterogeneity of ion signals. This method is generally applicable to various combinations of samples and matrices.

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“…Substrate (i.e., where the sample is placed) versus its surrounding temperatures can also affect heterogeneity, but this has been only applied to MALDI spots. 37 Automated sprayers have allowed reproducible application methods across individuals and laboratories, and as such, their popularity has grown in the past few years. 38 Several application notes from different vendors exist, but researchers should take time to optimize their application methods to their specific systems.…”

Section: Sample Preparationmentioning

confidence: 99%