Quantitative determination of perfluorooctanoic acid in serum and plasma by liquid chromatography tandem mass spectrometry

Flaherty, John M.; Connolly, Paul; Decker, Emily; Kennedy, Scott; Ellefson, Mark; Reagen, William K.; Szostek, Bogdan

doi:10.1016/j.jchromb.2005.03.002

Cited by 92 publications

(64 citation statements)

References 14 publications

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“…Several examples of the use of surrogate matrices for quantitation of exogenous and endogenous molecules have been reported. [28][29][30][31][32][33] We chose rabbit serum because initial observations indicated no interferences with either human or mouse hepcidin detection. Due to the difficulty of synthesizing human and mouse hepcidin, we were not able to use a stablelabeled internal standard that would have been preferred for development of this mass spectrometry-based assay.…”

Section: Resultsmentioning

confidence: 99%

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Murphy¹,

Witcher

Luan

et al. 2007

Blood

149

131

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The hepatic peptide hormone hepcidin is considered the central regulator of iron metabolism. Characterizing the circulating levels of this peptide is critical to understanding its role in the development of clinically relevant syndromes, such as anemia of inflammation/chronic disease, and may provide insight into potential clinical interventions. While quantitative methods have been published for the determination of urinary hepcidin and serum prohepcidin, no definitive methods have been published for the determination of hepcidin in serum. In this report, we describe a quantitative method for the determination of both human and mouse hepcidin in serum and plasma. The method employs protein precipitation and solid-phase extraction followed by liquid chromatographic separation and tandem mass spectrometry detection. The method has a quantitative range of 0.25 ng/mL to 500 ng/mL serum for mouse hepcidin and 1 ng/mL to 500 ng/mL serum for human hepcidin. The method uses small sample volumes (50 L for mice and 100 L for humans) and 96-well formats for rapid sample processing. The method was used to establish baseline serum and plasma concentrations of hepcidin in normal C57Bl/6 mice and healthy human volunteers. (Blood.

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Section: Resultsmentioning

confidence: 99%

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Murphy¹,

Witcher

Luan

et al. 2007

Blood

149

131

View full text Add to dashboard Cite

show abstract

“…The ion-pairing extraction using tetrabutylammonium salt and methyl tert-butyl ether was initially developed by Ylinen et al in 1985 [4]. Later, published methods for human matrices typically involve protein precipitation, using acid or acetonitrile followed by direct analysis [5] or additional clean-up with solid-phase extraction (SPE) [6][7][8], or dispersive carbon clean-up [9].…”

Section: Analytical Methodologymentioning

confidence: 99%

Accuracy and precision in the determination of perfluorinated chemicals in human blood verified by interlaboratory comparisons

Lindström

Kärrman

Bavel

2009

Journal of Chromatography A

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Perfluorinated chemicals, PFCs, are analyzed in laboratories worldwide to determine human blood levels and exposure pathways. The development of the analytical technique has been rapid in the last ten years, and prerequisites for accurate and precise determination of PFCs in human blood at low ng/g concentrations are today readily available. The main contributing factors are the improved LC-MS instrumentations, the increased availability of native and mass labeled PFC standards, and new column materials available for chromatographic separations. The results of the first international interlaboratory study (ILS) in 2005 on PFCs revealed relatively better analytical results for human blood analyses when compared to analyses of a number of environmental matrices. The representative accuracy for the analyses of PFCs in human matrixes reported in recent years was established in the second human serum ILS in 2006. Interlaboratory standard deviations for the two human serum samples one low level concentration and one medium level concentration were found to be 12% and 16% for PFOS, respectively, and 47% and 21% for PFOA, respectively. Reported detections for all PFCs followed a frequency of PFOS>PFOA>PFHxS>PFNA>PFDA>>PFDoA>> PFDS>>PFHxA. Due to the small number of reported values for the other perfluorosulfonates and perfluorocarboxylates, standard deviations were not established.

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“…Chromatographic and Mass spectrometric conditions-PFOA was analyzed through HPLC/tandem mass spectrometry by a slight modification of the method of Flaherty et al (22).…”

Section: Standards and Chemicals-thementioning

confidence: 99%

Community Exposure to Perfluorooctanoate: Relationships Between Serum Concentrations and Exposure Sources

Emmett

Shofer

Zhang

et al. 2006

Journal of Occupational and Environmental Medicine

318

300

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Objective-To determine serum [PFOA] in residents near a fluoropolymer production facility: the contributions from air, water and occupational exposures, personal and dietary habits, and relationships to age and gender.Methods-Questionnaire and serum PFOA measurements in a stratified random sample and volunteers residing in locations with the same residential water supply but with higher and lower potential air PFOA exposure. [PFOA] greatly exceeded general population medians. Occupational exposure from production processes using PFOA and residential water had additive effects, no other occupations contributed. Serum [PFOA] depended on the source of residential drinking water, and not potential air exposure. For public water users the best-fit model included age, tap water drinks per day, servings of home-grown fruit and vegetables, and carbon filter use. Results-SerumConclusions-Residential water source was the primary determinant of serum [PFOA].

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Quantitative determination of perfluorooctanoic acid in serum and plasma by liquid chromatography tandem mass spectrometry

Cited by 92 publications

References 14 publications

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Accuracy and precision in the determination of perfluorinated chemicals in human blood verified by interlaboratory comparisons

Community Exposure to Perfluorooctanoate: Relationships Between Serum Concentrations and Exposure Sources

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