Quantitative determination of osteopontin in bovine, buffalo, yak, sheep and goat milk by Ultra-high performance liquid chromatography-tandem mass spectrometry and stable isotope dimethyl labeling

Hu, Bei; Zhang, Jingshun; Jiang, Yifan; Tong, Wenfeng; Lai, Shiyun; Ren, Yiping

doi:10.1016/j.foodchem.2020.128489

Cited by 7 publications

(7 citation statements)

References 32 publications

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“…However, data acquired from different commercially ELISA kits showed variable and non-comparable absolute concentrations (30). Moreover, the ELISA method is of concern since cross-reactivity or non-specific antibody recognition have been noticed in complex sample matrix and lead to false negative or false positive (31). Such problems have been observed in the article (28) mentioned that a study (5) detected LPN levels using an ELISA that was not validated for use in human milk, the detection levels of LPN was shown to overestimate up to 10-fold from the actual levels.…”

Section: Discussionmentioning

confidence: 99%

Longitudinal changes of lactopontin (milk osteopontin) in term and preterm human milk

Zhu

Bai

et al. 2022

Front. Nutr.

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BackgroundLactopontin (LPN) in breast milk, also known as milk osteopontin is thought to play a myriad of important roles in infants when they are immature. The purpose of the present study was to examine the longitudinal changes in LPN concentrations in term and preterm milk, and elucidate the links between maternal characteristics, LPN levels, and child growth in a birth cohort.Methods131 mothers who delivered term, moderate-late preterm (MPT), very preterm (VPT), and extremely preterm (EPT) infants were included, milk samples were collected at 7, 14, 28, and 120 days postpartum. LPN concentration was determined by multiple reaction monitoring (MRM) using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).ResultsOur results indicated that LPN change over time of VPT (P = 0.024) and EPT (P = 0.003) were significantly different from term milk, although they all gradually decreased with lactation. In terms of LPN-related factors, maternal age was a significant contributor in late mature milk and pre-pregnancy BMI a significant contributor to colostrum and transitional milk. We further investigated relationships between LPN levels and infant weight and our results suggested that high levels of LPN in breast milk might be useful for the catch-up growth of infants.ConclusionLPN levels in breast milk are related to maternal factors, and differences in LPN levels may affect the growth of infants. As milk is a critical part in the mother–breastmilk–infant “triad,” the association between maternal-infant factors and milk LPN levels warrants further study.

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Section: Discussionmentioning

confidence: 99%

Longitudinal changes of lactopontin (milk osteopontin) in term and preterm human milk

Zhu

Bai

et al. 2022

Front. Nutr.

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“…A study using UHPLC-MS/MS (ultra-high-performance liquid chromatography tandem mass spectrometry) estimated the OPN concentrations in milk from cow (Bos taurus), buffalos (Bison bison), sheep (Ovis aries), goats (Capra hircus), and yaks (Bos grunniens). Two measurements were reported for all species: cows (51.4 mg/L and 56.4 mg/L), buffalos (68.5 mg/L and 51.8 mg/L), yaks (78.6 mg/L and 76.8 mg/L), sheep (41.06 mg/L and 29.8 mg/L), and goats (44.3 mg/L and 12.7 mg/L) [27]. A comprehensive proteomic study of camel milk showed great seasonal changes in the concentration of OPN, with a 50% increase in OPN levels from winter to summer [28].…”

Section: Osteopontin In Milkmentioning

confidence: 99%

Milk Osteopontin and Human Health

Sørensen

Christensen

2023

Nutrients

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Osteopontin (OPN) is a multifunctional protein found in all vertebrates. OPN is expressed in many different cell types, and is consequently found in most tissues and physiological secretions. OPN is involved in a multitude of biological processes, such as activation and regulation of the immune system; biomineralization; tissue-transformative processes, including growth and development of the gut and brain; interaction with bacteria; and many more. OPN is found in the highest concentrations in milk, where it is believed to initiate and regulate developmental, immunological and physiological processes in infants who consume milk. Processes for the isolation of bovine OPN for use in infant formula have been developed, and in recent years, many studies have investigated the effects of the intake of milk OPN. The purpose of this article is to review and compare existing knowledge about the structure and function of milk OPN, with a particular focus on the effects of milk OPN on human health and disease.

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“…Enzyme-linked immunosorbent assay (ELISA) , and liquid chromatography coupled with mass spectrometry (LC-MS) , are the two analytical approaches to determine the OPN concentration in dairy products. ELISA has been mostly applied in the detection of human OPN in breast milk because of its easy procedures.…”

Section: Introductionmentioning

confidence: 99%

Analytical Ultracentrifugation-Calibrated Anion-Exchange Chromatography for Sensitive and Intact Determination of Osteopontin in Infant Formula and Dairy Products

Wang,

Cui,

et al. 2023

J. Agric. Food Chem.

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Osteopontin is a crucial protein ingredient that has been applied in fortified dairy products and infant formula. It has great significance to infant gut health and brain development. However, current techniques including enzyme-linked immunosorbent assay and liquid chromatography coupled with mass spectrometry are still facing the bottleneck of low sensitivity and indirect quantification. Moreover, the unavailable certified commercial OPN standard hinders its accurate quantification. Herein, a novel method of anion-exchange chromatography was established to determine OPN concentration in several dairy matrices. The polarity-reversed capillary isoelectric focusing was utilized to measure the exact isoelectric point (pI) to support method development for OPN separation. Analytical ultracentrifugation was used to calibrate the purity of intact OPN to develop an in-house reference standard. The method showed the merits of limits of detection to 0.04 mg/100 g, relative standard deviation of reproducibility <5% for 13 out of 14 tested matrices, and an average recovery rate of 101.3%. This method has shown the potential to be adopted as an international standard method for the quantification of intact OPN in infant formula and dairy products.

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Quantitative determination of osteopontin in bovine, buffalo, yak, sheep and goat milk by Ultra-high performance liquid chromatography-tandem mass spectrometry and stable isotope dimethyl labeling

Cited by 7 publications

References 32 publications

Longitudinal changes of lactopontin (milk osteopontin) in term and preterm human milk

Longitudinal changes of lactopontin (milk osteopontin) in term and preterm human milk

Milk Osteopontin and Human Health

Analytical Ultracentrifugation-Calibrated Anion-Exchange Chromatography for Sensitive and Intact Determination of Osteopontin in Infant Formula and Dairy Products

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