Quantitative Determination of Organic Nitrogen in Water, Sewage, and Industrial Wastes

Morgan, G. B.; Lackey, James B.; Gilcreas, F. W.

doi:10.1021/ac60125a029

Cited by 27 publications

(6 citation statements)

References 2 publications

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“…Nitrogen was determined according to the Kjeldahl method (Morgan et al 1957). Total nitrogen concentration and C/N ratio were calculated according to the published equations (Gong et al 2016b).…”

Section: Methodsmentioning

confidence: 99%

Feasibility of Lipid Production from Waste Paper by the Oleaginous Yeast Cryptococcus curvatus

et al. 2017

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Waste paper was studied as a potential source for lipid production using the oleaginous yeast Cryptococcus curvatus for the first time. Three common types of waste paper, office paper, newspaper, and cardboard, were directly hydrolyzed by an enzyme cocktail to generate sugar-rich and nitrogen-limited hydrolysates. When these hydrolysates were used without any auxiliary nutrients by C. curvatus, the lipid content and lipid yield were higher than 50% and 200 mg/g, respectively. The nitrogen-rich enzyme cocktail exerted no negative effects on lipid production. Moreover, the integrated processes of enzymatic hydrolysis and lipid fermentation achieved comparable lipid yield to the separate hydrolysis and lipid production process. The resulting lipid samples had similar fatty acid compositional profiles to those of vegetable oils, which suggested their potential for biodiesel production. These findings strongly supported waste paper as appealing substrates for lipid production via oleaginous yeast, which provided cost-effective waste paper-to-lipids routes for sustainable biodiesel production.

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Section: Methodsmentioning

confidence: 99%

Feasibility of Lipid Production from Waste Paper by the Oleaginous Yeast Cryptococcus curvatus

et al. 2017

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“…Total nitrogen was quantified by estimation of the ammonia formed with a KDN-2C type nitrogen installation (Shanghai, China) according to a standard method [19].…”

Section: Analytic Methodsmentioning

confidence: 99%

Production of lipid from N‐acetylglucosamine by Cryptococcus curvatus

Zhao

et al. 2010

Euro J Lipid Sci & Tech

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N-Acetylglucosamine (GlcNAc), the monomeric constituent of chitin, is rarely used as a carbon source for fermentation technology. In this study, we demonstrate that the oleaginous yeast Cryptococcus curvatus ATCC 20509 can produce intracellular lipid during the cultivation process and total lipid content can reach 54% on a GlcNAc-based medium. Culture of C. curvatus under various conditions indicated that lipid accumulation also occurred at a relatively broad range of temperatures as well as relatively high initial GlcNAc concentrations. Fatty acid analysis indicated that the product was rich in palmitic acid, stearic acid, and oleic acid, closely resembling the composition of palm oil. More importantly, the lipid sample produced at 22 8C had a total saturated fatty acid content of 54.2 wt%, suggesting that it may be explored as cocoa-butter equivalent. Our data suggested that GlcNAc could be used as a feedstock for industrial biotechnology and that C. curvatus ATCC 20509 is a strain capable of accumulating high intracellular lipid using this nitrogen-rich renewable material.Practical applications: Microbial lipid is a versatile material, especially for biodiesel production. Stable and abundant renewable raw substrates remain to be explored for large-scale production of microbial lipid. The present work reports lipid production using N-acetylglucosamine (GlcNAc) by the oleaginous yeast Cryptococcus curvatus ATCC 20509 to yield up to 54% intracellular lipid content. More significantly, the lipid sample produced at 22 8C had a total saturated fatty acid content of 54.2 wt%, suggesting that it may be explored as cocoa-butter equivalent. Our technology provides the opportunity to effectively convert GlcNAc, available from one of the most abundant renewable materials chitin, into lipid. This procedure should prove valuable in terms of renewable energy production as well as environmental pollution control.

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“…29 Total nitrogen was analyzed using the modified Kjeldahl-mercuric sulfate method, 30 while ammonia nitrogen was determined by the Nessler's reagent colorimetric method. 31 To analyze total carbohydrates, the method of centrifugation with formaldehyde was applied to extract carbohydrates in the sludge, 32 and subsequently the phenol-sulfuric acid method was carried out with glucose as a standard in the range 10-70 mg L −1 .…”

Section: Analysesmentioning

confidence: 99%

Effects of organic matter and initial carbon–nitrogen ratio on the bioconversion of volatile fatty acids from sewage sludge

Liu

Chen

et al. 2008

J of Chemical Tech & Biotech

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Quantitative Determination of Organic Nitrogen in Water, Sewage, and Industrial Wastes

Cited by 27 publications

References 2 publications

Feasibility of Lipid Production from Waste Paper by the Oleaginous Yeast Cryptococcus curvatus

Feasibility of Lipid Production from Waste Paper by the Oleaginous Yeast Cryptococcus curvatus

Production of lipid from N‐acetylglucosamine by Cryptococcus curvatus

Effects of organic matter and initial carbon–nitrogen ratio on the bioconversion of volatile fatty acids from sewage sludge

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