Quantitative Determination of Milnacipran HCL in Rabbit Plasma by HPLC and Its Application to Pharmacokinetics Study

Parejiya, Punit B.; Movaliya, Vinit; Barot, Bhavesh S.; Modi, Darshana K.; Shelat, Pragna K.; Shukla, Arunkumar

doi:10.1080/10826076.2012.734002

Cited by 12 publications

(7 citation statements)

References 15 publications

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“…The validation of an analytical method authenticates the parameters of the method for application (Parejiya et al, 2014). The method used for the determination of venlafaxine HCl was validated for precision, accuracy, linearity, sensitivity and robustness.…”

Section: Methods Validationmentioning

confidence: 89%

“…The linearity was evaluated by linear regression analysis. Quantitative analytical results are greatly affected by the quality of the calibration curve (Parejiya et al, 2014). The calibration curve was obtained with seven concentrations of reference standard venlafaxine HCl solution (10, 50, 100, 200, 300, 400 and 500 μg mL −1 ) of the target analyte concentrations for the chromatographic method.…”

Section: Linearitymentioning

confidence: 99%

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Development and validation of an HPLC-UV method for accelerated stability study and pharmacokinetic analysis of venlafaxine

Sher

Ahmad

Hassan

et al. 2020

Braz. J. Pharm. Sci.

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A reverse phase high performance liquid chromatography method has been developed and validated for accelerated stability study and determination of pharmacokinetic parameters of venlafaxine HCl. The chromatographic separation was carried out using ODS analytical column (250 × 4.6 mm i.d., 5 μm particle size). The mobile phase included acetonitrile, methanol and potassium dihydrogen phosphate buffer (30:30:40; pH 6.1) at a flow rate 1.5 mL min −1 . UV-Visible detector was used at wavelength of 227 nm to monitor elutions. Retention time observed was 2.745 min. The method was validated for linearity, accuracy, precision, sensitivity and robustness. Accelerated stability study of venlafaxine HCl capsules was carried out at 40 and 50 °C under 75% RH level. Suggested method was successfully applied for the pharmacokinetic analysis of venlafaxine hydrochloride tablets. Each of ten albino rabbits (≈ 1.2 kg each) was orally administered with 5 mg dose of venlafaxine HCl. The method was proved to be linear (R 2 >0.998), accurate (98.25-99.27%), sensitive (LOD: 35ngmL −1 ; LOQ: 105 ng mL −1 ) and robust (RSD<1%). The drug showed stability at accelerated conditions of temperature and humidity. The main pharmacokinetic parameters of tested products were as follows: t max was 2.5h, C max was 56.5 μg mL −1 , t 1/2 was 8.2 h, AUC 0-36 was 845.9 μg h mL −1 . The developed method is suitable to apply for quality control analysis and pharmacokinetic studies.

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Section: Methods Validationmentioning

confidence: 89%

Section: Linearitymentioning

confidence: 99%

Development and validation of an HPLC-UV method for accelerated stability study and pharmacokinetic analysis of venlafaxine

Sher

Ahmad

Hassan

et al. 2020

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

show abstract

“…4 It acts by unique and equal inhibition of noradrenaline and serotonin neurotransmitters reuptake. 5 In the previous analytical literature, MLC was assayed by various techniques which include GC-MS, 6 liquid chromatography with uorescence or UV detection, 7,8 spectrouorimetry by derivatization with ninhydrin 9 or NBD-Cl, 10 UVspectrophotometric detection at 223 nm 11,12 and colorimetry using ninhydrin 13 or ion pair complex. 14 The approach target was simple development for accurate assay of MLC in the spiked biological uid, and tablets.…”

Section: Introductionmentioning

confidence: 99%

A new approach based on isoindole formation reaction for sensitive fluorimetric assay of milnacipran in tablets and biological fluids (plasma/urine)

2020

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“…[ 6 ] However, the developed method still suffered from low sensitivity (LOQ = 1.67 μg ml −1 ) and tedious procedures as it required heating at high temperature to 120°C using a glycerol bath. Spectrofluorimetric, [ 7–9 ] chromatographic [ 10–19 ] and capillary electrophoretic methods [ 20 ] were reported for assay of MCP. The reported spectrofluorimetric methods [ 7,8 ] used an expensive fluorogenic reagent that has a high fluorescence background and required acidification with high concentrations of hydrochloric acid to decrease reagent interference.…”

Section: Introductionmentioning

confidence: 99%

Use of Hantzsch reaction for quantitation of milnacipran as a magic treatment for fibromyalgia syndrome in human plasma and urine

et al. 2020

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A new fluorimetric procedure is described for analysis of milnacipran in its bulk, tablet dosage forms, as well as in biological human samples such as plasma and urine. The suggested method relies on the construction of a derivative with strong fluorescence called dihydropyridine derivative. This derivative resulted from the interaction of the primary amino group in the studied drug and acetylacetone/formaldehyde in McIlvaine buffer (pH 5). The fluorescent dihydropyridine derivative was measured at 470 nm. Influences of experimental variables namely pH, reagent concentration and temperature were examined and optimized. The calibration curve showed linearity over the range of 0.15–1.25 μg ml−1 of milnacipran with an R2 value of 0.9998. The detection limit was 0.02 μg ml−1 and the determination limit was 0.07 μg ml−1. The developed procedure was successfully used in the assay of the studied drug in Avermilan® tablets with excellent selectivity. In addition, the reaction was applied to estimate the drug in spiked human plasma and urine with mean percentage recoveries of 100.04 ± 1.61 and 99.78 ± 0.81% for urine and plasma, respectively.

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Quantitative Determination of Milnacipran HCL in Rabbit Plasma by HPLC and Its Application to Pharmacokinetics Study

Cited by 12 publications

References 15 publications

Development and validation of an HPLC-UV method for accelerated stability study and pharmacokinetic analysis of venlafaxine

Development and validation of an HPLC-UV method for accelerated stability study and pharmacokinetic analysis of venlafaxine

A new approach based on isoindole formation reaction for sensitive fluorimetric assay of milnacipran in tablets and biological fluids (plasma/urine)

Use of Hantzsch reaction for quantitation of milnacipran as a magic treatment for fibromyalgia syndrome in human plasma and urine

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