Quantitative Determination of Mast Cell Fragmentation by Compound 48/80

Abstract

Compound 48/80 as reported by Baltzly, Buck, de Beer, and Webb (1949) All solutions were at room temperature. Usually two pieces of mesentery were used in each concentration of drug. From these pieces five microscope fields were selected at random under 100 x magnification from widely separated areas of the mesentery. In each field, using 430 x magnification, the first 10 mast cells were examined, starting from the lefthand side of the field and proceeding clockwise. For each drug concentration 50 cells were counted and the percentage of disrupted cells was obtained. Each cell was considered either "disrupted" or ".not disrupted." The term " disrupted " was selected instead of " fragmented," since granules were found around many cells which did not appear to be in fragments. Fawcett (1954) has also reported this phenomenon. The sole criterion for calling a cell " disrupted " was the presence of granules outside the cell. Many cells which did not show extrusion of granules appeared swollen at low concentrations of 48/80.For evaluating the percentage disruption the labels of the slides were covered and the slides were counted in random sequence. RESULTS Comparison

Evaluation of antihistaminic Activity of herbal drug isolated from Cuscuta reflexa Roxb.

“…After an incubation period of 10 minutes, the tissues were removed, trimmed and stained with 1.0% toluidine blue solution and examined microscopically for the number of intact and de-granulated mast cells. A mast cell was considered disrupted if four or five granules were observed around the mast cells as reported by Norton (1954).…”

In vitro studies on anti asthmatic, analgesic and anti convulsant activities of the medicinal plant Bryonia laciniosa.Linn

“…The tissue was first immersed in 0.1% toludine blue (in 4% aqueous saline) for 10 minutes [12]. 10.The tissue was then treated with xylene for 5-10 minutes.…”

Peritoneal Mast Cell Stabilization Potential of Ziziphus Xylopyrus (Retz) Wild Extract in Rat Mesenteric Model

“…[19] In the present investigation, the authors have tried to assess the in vitro mast cell stabilization studies of the extracts ZXE and ZXA observed a significant dose dependant reduction (p<0.001) in mast cell degranulation when compared to C48/80. ZXE extracts (68.8 ± 2.7) demonstrated dose dependent highest mast cell stabilization ability in rat mesentery preparation.…”

“…A mast cell was considered disrupted if 4 or 5 granules were found around the mast cells. The percentage of mast cell fragmented or disrupted and of intact mast cells was calculated [18,19].…”