Quantitative determination of juvenile hormone III and 20-hydroxyecdysone in queen larvae and drone pupae of Apis mellifera by ultrasonic-assisted extraction and liquid chromatography with electrospray ionization tandem mass spectrometry

Zhou, Jinhui; Qi, Yitao; Hou, Yu; Zhao, Jing; Li, Yi; Xue, Xiaofeng; Wu, Liming; Zhang, Jinzhen; Chen, Fang

doi:10.1016/j.jchromb.2011.07.006

Cited by 49 publications

(35 citation statements)

References 26 publications

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“…Further activation of m/z = 235 and 249 generates m/z = 217 [M+H-CH 3 OH-H 2 O] + and m/z = 207 [M+H-CH 3 OH-CO] + , corresponding to the combined loss of one methanol group and water, and to the loss of a CO group, respectively. In contrast to previous reports(25), our data support the generation of m/z = 217 as a product of 267->235->217 and 267->249->217. The activation of m/z = 217 produces m/z = 189 [M+H-CH 3 OH-H 2 O-CO] + , which further fragments into m/z = 147 [M+H-CH 3 OH-H 2 O-CO-CH 3 CH=CH 2 ] + .…”

Section: Resultscontrasting

confidence: 99%

“…That is, the most abundant fragmentation transitions utilized were 267->235 (primary) and 267-> 147 (secondary). Previous reports have also utilized 267-> 235 as a primary and 267-> 217 as secondary MRM transitions for LC-MS/MS analysis(25, 27). …”

Section: Resultsmentioning

confidence: 99%

“…There were no significant differences in terms of recovery relative to the initial measured concentration (see supplemental Tables S1 and S2) as a function of storage time, suggesting that dried biological samples can be correctly quantified after a month of dry storage. Previous work has shown that JH III is stable for up to one month and up to six 6 months in methanol if stored at 4°C and −18°C, respectively(25). Similar stabilities were detected when JH III was stored in hexane and acetonitrile.…”

Section: Resultsmentioning

confidence: 99%

“…A variety of ionization sources have been utilized prior to the MS analysis, such as electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), atmospheric pressure photoionization (APPI) and atmospheric pressure thermospray ionization (APTSI); allowing the detection of the JH III molecular ion in the protonated and sodiated forms(33). While some studies showed the advantages of using the sodiated species for quantification in single ion monitoring mode (SIM), only the protonated species allowed for multiple reaction monitoring (MRM)(25). …”

Section: Introductionmentioning

confidence: 99%

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Fast, ultra-trace detection of juvenile hormone III from mosquitoes using mass spectrometry

Ramírez

Nouzová

Benigni

et al. 2016

Talanta

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In the present work, a new protocol for fast separation and quantification of JH III from biological samples using liquid chromatography coupled to electrospray tandem mass spectrometry is described. In particular, the proposed protocol improves existing methodologies by combining a limited number of sample preparation steps with fast LC-MS/MS detection, providing lower limits of detection and demonstrated matrix effect control, together with high inter and intraday reproducibility. A limit of detection of 8 pg/mL (0.32 pg on column) was achieved, representing a 15-fold gain in sensitivity with respect to previous LC-MS based protocols. The performance of the LC-MS/MS protocol is comparable to previously described JH III quantitation protocol based on fluorescence detection, with the added advantage that quantification is independent of the availability of fluorescent tags that are often unavailable or show quite diverse responses on a batch-to-batch basis. Additionally, a detailed description of the JH III fragmentation pathway is provided for the first time, based on isolation of the molecular ion and their intermediate fragments using in-source MS/MS, MS/MSn and FT-ICR MS/MS measurements. The JH III workflow was evaluated as a function of developmental changes, sugar feeding and farnesoic acid stimulation in mosquitoes and can be applied to the detection of other juvenile hormones.

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Section: Resultscontrasting

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fast, ultra-trace detection of juvenile hormone III from mosquitoes using mass spectrometry

Ramírez

Nouzová

Benigni

et al. 2016

Talanta

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“…The liquid chromatography mass spectrometry ecdysone analyses were performed with the liquid chromatography instrument UHPLC Agilent Technologies 1290 Infinity (Santa Clara, California, USA) in combination with an Agilent triple quadruple 6430 mass spectrometer with electrospray ionization (Santa Clara, California, USA) (adapted from [31,32]). The system operation was controlled and processed with the software MassHunter B06.00.…”

Section: Methodsmentioning

confidence: 99%

Proximate mechanism of behavioral manipulation of an orb-weaver spider host by a parasitoid wasp

et al. 2017

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Some ichneumonid wasps induce modifications in the web building behavior of their spider hosts to produce resistant “cocoon” webs. These structures hold and protect the wasp’s cocoon during pupa development. The mechanism responsible for host manipulation probably involves the inoculation of psychotropic chemicals by the parasitoid larva during a specific developmental period. Recent studies indicate that some spiders build cocoon webs similar to those normally built immediately before ecdysis, suggesting that this substance might be a molting hormone or a precursor chemical of this hormone. Here, we report that Cyclosa spider species exhibiting modified behavior presented higher 20-OH-ecdysone levels than parasitized spiders acting normally or unparasitized individuals. We suggest that the lack of control that spiders have when constructing modified webs can be triggered by anachronic activation of ecdysis.

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IDENTIFICATION AND HORMONE INDUCTION OF PUTATIVE CHITIN SYNTHASE GENES AND SPLICE VARIANTS IN Leptinotarsa decemlineata (SAY)

Shi

Guo

et al. 2016

Arch Insect Biochem Physiol

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Chitin synthase (ChS) plays a critical role in chitin synthesis and excretion. In this study, two ChS genes (LdChSA and LdChSB) were identified in Leptinotarsa decemlineata. LdChSA contains two splicing variants, LdChSAa and LdChSAb. Within the first, second, and third larval instars, the mRNA levels of LdChSAa, LdChSAb, and LdChSB coincide with the peaks of circulating 20-hydroxyecdysone (20E) and juvenile hormone (JH). In vitro culture of midguts and an in vivo bioassay revealed that 20E and an ecdysteroid agonist halofenozide stimulated the expression of the three LdChSs. Conversely, a reduction of 20E by RNA interference (RNAi) of an ecdysteroidogenesis gene LdSHD repressed the expression of these LdChSs, and ingestion of halofenozide by LdSHD RNAi larvae rescued the repression. Moreover, disruption of 20E signaling by RNAi of LdEcR, LdE75, LdHR3, and LdFTZ-F1 reduced the expression levels of these genes. Similarly, in vitro culture and an in vivo bioassay showed that exogenous JH and a JH analog methoprene activated the expression of the three LdChSs, whereas a decrease in JH by RNAi of a JH biosynthesis gene LdJHAMT downregulated these LdChSs. It seems that JH upregulates LdChSs at the early stage of each instar, whereas a 20E pulse triggers the transcription of LdChSs during molting in L. decemlineata.

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Quantitative determination of juvenile hormone III and 20-hydroxyecdysone in queen larvae and drone pupae of Apis mellifera by ultrasonic-assisted extraction and liquid chromatography with electrospray ionization tandem mass spectrometry

Cited by 49 publications

References 26 publications

Fast, ultra-trace detection of juvenile hormone III from mosquitoes using mass spectrometry

Fast, ultra-trace detection of juvenile hormone III from mosquitoes using mass spectrometry

Proximate mechanism of behavioral manipulation of an orb-weaver spider host by a parasitoid wasp

IDENTIFICATION AND HORMONE INDUCTION OF PUTATIVE CHITIN SYNTHASE GENES AND SPLICE VARIANTS IN Leptinotarsa decemlineata (SAY)

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