Fast, ultra-trace detection of juvenile hormone III from mosquitoes using mass spectrometry

Ramírez, César E.; Nouzová, Marcela; Benigni, Paolo; Quirke, J. Martin E.; Noriega, Fernando G.; Fernandez-Lima, Francisco

doi:10.1016/j.talanta.2016.06.041

Cited by 16 publications

(18 citation statements)

References 35 publications

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“…A 10 μl aliquot of deuterated JHIII analog (6.25 ppb) in acetonitrile was added to each sample before extraction with 600 μl hexane. The organic phase was dried under a stream of nitrogen and resuspended in 50 μl of acetonitrile and subjected to analysis by HPLC-MS/MS analyses [39].…”

Section: Juvenile Hormone III and Triglyceride Quantification In Wt Amentioning

confidence: 99%

A mosquito juvenile hormone binding protein (mJHBP) regulates the activation of innate immune defenses and hemocyte development

et al. 2020

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Section: Juvenile Hormone III and Triglyceride Quantification In Wt Amentioning

confidence: 99%

A mosquito juvenile hormone binding protein (mJHBP) regulates the activation of innate immune defenses and hemocyte development

et al. 2020

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“…We have previously described the potential of mass spectrometry (MS) based protocols for structural identification and quantification of JH homologues from insect samples 11,12 . More recently, we reported on a liquid chromatography-mass spectrometry (LC-MS/MS) protocol for the identification and quantification of the five most common JH homologs 13 .…”

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confidence: 99%

The juvenile hormone described in Rhodnius prolixus by Wigglesworth is juvenile hormone III skipped bisepoxide

Villalobos-Sambucaro

Nouzová

Ramírez

et al. 2020

Sci Rep

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Juvenile hormones (JHs) are sesquiterpenoids synthesized by the corpora allata (CA). They play critical roles during insect development and reproduction. The first JH was described in 1934 as a "metamorphosis inhibitory hormone" in Rhodnius prolixus by Sir Vincent B. Wigglesworth. Remarkably, in spite of the importance of R. prolixus as vectors of Chagas disease and model organisms in insect physiology, the original JH that Wigglesworth described for the kissing-bug R. prolixus remained unidentified. We employed liquid chromatography mass spectrometry to search for the JH homologs present in the hemolymph of fourth instar nymphs of R. prolixus. Wigglesworth's original JH is the JH III skipped bisepoxide (JHSB3), a homolog identified in other heteropteran species. Changes in the titer of JHSB3 were studied during the 10-day long molting cycle of 4 th instar nymph, between a blood meal and the ecdysis to 5 th instar. In addition we measured the changes of mRNA levels in the CA for the 13 enzymes of the JH biosynthetic pathway during the molting cycle of 4 th instar. Almost 90 years after the first descriptions of the role of JH in insects, this study finally reveals that the specific JH homolog responsible for Wigglesworth's original observations is JHSB3.Sir Vincent B. Wigglesworth, the famous British insect endocrinologist, published a series of three classical manuscripts in the years 1934, 1936 and 1940 that described the existence of an "inhibitory hormone" that prevented metamorphosis in the early nymphal stages of the blood-sucking bug Rhodnius prolixus 1-3 . The manuscripts contained a series of elegant studies, including a parabiosis techniques that allowed the mixing of the hemolymph of fourth and fifth instar nymphs, as well as corpora allata (CA) transplantation experiments. These studies permitted Wigglesworth to demonstrate for the first time the existence of an "inhibitory hormone" secreted by the CA and delivered to target tissues by the hemolymph. In his latest article, Wigglesworth proposed for the first time to name the "inhibitory hormone" as "juvenile hormone" (JH) 3 . Wigglesworth also used transplantation and parabiosis experiments to reveal that the CA of the 4 th instar nymphs of R. prolixus was an equally good source of a "yolk-forming hormone", whereas the CA of a 5 th instar nymph was ineffective. He concluded that only a single hormone, the "Juvenile Hormone", was responsible for both actions, the inhibition of metamorphosis, as well as the control of development of the reproductive organs in the adult 4 .After the pioneering studies by Wigglesworth and others, the chemical nature of JH remained unidentified until the late 1960's. The first two JHs, named JH I and II, were isolated from the moth Hyalophora cecropia. They were identified as sesquiterpenes, with a methyl ester (α, β-unsaturated) at the C1 position and an epoxide ring at the C10-C11 position 5,6 . Juvenile hormone III, the homolog found in most insects, was described in 1973 from the moth Manduca sexta 7 . Finally, two dou...

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“…Hemolymph was obtained by perfusion 13 , and JH III titers were measured using a high performance liquid chromatography coupled to electrospray tandem mass spectrometry approach (HPLC-MS/MS) 14 . The changes in the titers of JH detected in hemolymph of sugar-fed females after adult emergence, as well as following a blood meal are presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

Juvenile hormone controls ovarian development in female Anopheles albimanus mosquitoes

Hernández‐Martínez

Cardoso-Jaime

Nouzová

et al. 2019

Sci Rep

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Anophelinae mosquitoes are vectors of human malaria, a disease that infects hundreds of millions of people and causes almost 600,000 fatalities annually. Despite their medical importance, laboratory studies on key aspects of Anophelinae reproductive biology have been limited, and in particular, relatively little is known about the role of juvenile hormone (JH) in the control of female reproduction. The study presented here attempts to fill a gap of knowledge in our understanding of the JH control of ovarian development in female Anophelinae mosquitoes, using Anopheles albimanus as a model. Our studies revealed that JH controls the tempo of maturation of primary follicles in An. albimanus in a similar manner to that previously described in Aedes aegypti. At adult eclosion JH hemolymph titer was low, increased in 1-day old sugar-fed insects, and decreased in blood fed individuals. JH titers decreased if An. albimanus females were starved, and were reduced if insects emerged with low teneral reserves, precluding previtellogenic ovarian development. However, absolute hemolymph titers were lower than Ae. aegypti. Decapitation experiments suggested that if teneral reserves are sufficient, factors from the head activate JH synthesis by the corpora allata (CA) during the first 9–12 h after adult emergence. In conclusion, our studies support the hypothesis that JH controls previtellogenic ovarian development in female An. albimanus mosquitoes, in a similar manner that have been described in Culicinae.

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Fast, ultra-trace detection of juvenile hormone III from mosquitoes using mass spectrometry

Cited by 16 publications

References 35 publications

A mosquito juvenile hormone binding protein (mJHBP) regulates the activation of innate immune defenses and hemocyte development

A mosquito juvenile hormone binding protein (mJHBP) regulates the activation of innate immune defenses and hemocyte development

The juvenile hormone described in Rhodnius prolixus by Wigglesworth is juvenile hormone III skipped bisepoxide

Juvenile hormone controls ovarian development in female Anopheles albimanus mosquitoes

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