Quantitative determination of gibberellins by high performance liquid chromatography from various gibberellins producing Fusarium strains

Abstract

High performance liquid chromatographic (HPLC) method was developed for analysis of seven gibberellins, i.e., GA3, GA4, GA7, GA3 methyl ester, GA7 methyl ester 3,13 diacetate, GA7 methyl ester, and fusaric acid, using an isocratic system. Method was used for estimation of gibberellins from different Fusarium strains. Gibberellins were extracted from 28 strains of Fusarium, out of which six strains of Fusarium were isolated from soil of different parts of India and 22 strains were procured from the Indian Type Culture Collection, Indian Agricultural Research Institute, New Delhi. Extracts were analyzed for qualitative and quantitative estimation of gibberellins by thin layer chromatography and HPLC, respectively. On the basis of quantitative analysis of produced gibberellins by HPLC, they were categorized as low, moderate, and high gibberellin producing strain. For the first time, Fusarium solani was also reported as high GA3 producing strain.

Light-Dependent Functions of the Fusarium fujikuroi CryD DASH Cryptochrome in Development and Secondary Metabolism

“…Gibberellins (GAs) were extracted from 0.5 ml of acidified filtered medium and run in parallel to a GA3 standard on thin-layer chromatography (TLC) as described previously (39). The samples were dried, dissolved in 6 l acetonitrile, loaded on silica gel 60 TLC plastic sheets (Merck, Darmstadt, Germany), and run in benzene-n-butyl alcoholacetic acid (60:30:10) as the mobile phase.…”

ESTANDARIZACIÓN DE UN MÉTODO CROMATOGRÁFICO PARA LA IDENTIFICACIÓN DEL ÁCIDO GIBERÉLICO EN SEMILLAS DE MAÍZ (Zea mays L.)

“…hasta 0.8 mL min -1 . Para determinar la fase móvil óptima, se realizaron pruebas basadas en los trabajos de Bhalla et al, [13], Xie et al, [21] y Stinemetz y Roberts [6], los cuales realizaron corridas cromatográficas en modo isocrático, empleando mezclas de acetonitrilo-ácido fórmico 0.01% y metanolácido fórmico 0.01%, a flujos de 0.5 mL min -1 . y 0.6 mL min-1.…”

“…Bhalla et al, [13] y Xie et al, [21], ya habían estudiado un tipo similar de fases móviles para separar el GA 3 en muestras vegetales, logrando buena separación y obteniendo el analíto en estudio con tiempo de retención de 3.651 min. Sin embargo, en este estudio no se logró una apropiada separación del GA 3 bajo estas condiciones, esto tal vez debido a la complejidad de la matriz de estudio; evidenciada en el amplio intervalo de compuestos con diferente polaridad (Figura 1); por lo que fue necesario evaluar fases móviles en gradiente.…”

“…Bajo estas condiciones se encontró al patrón de ácido giberélico (GA 3 ) a una longitud de onda máxima de absorción de 195 nm. ; por debajo de lo reportado por Macías et al, [14], Bhalla et al, [13] La curva de Van Deemter (Figura 2) muestra la relación entre la velocidad lineal (u) de la fase móvil y la altura equivalente de plato teórico (HETP). En esta se observa que la menor altura de equivalente de plato teórico (0.0025 mm.)…”

New 3-<i>O</i>-Alkyl-4a,10a-dihydrofusarubins Produced by <i>Fusarium</i> sp. Mj-2

“…1,2) The Fusarium fungus is a well-known soil-borne saprophytic and parasitic fungus that produces diverse bioactive secondary metabolites; for example, fumonisins, trichothecenes and zearalenone are mycotoxins, 3) fusaric acid and moniliformin are phytotoxins, 4) and gibberellins are phytohormones. 5) A number of Fusarium fungi also produce naphthoquinones and related metabolites as fungal pigments; they include javanicin, fusarubin, anhydrofusarubin, 4a,10a-dihydrofusarubin, and marticin. 6) Among them, 4a,10a-dihydrofusarubins A and B were first isolated in 1978 from F. solani as true metabolites before non-enzymatic oxidation to give fusarubin, 7) and the 3-O-ethyl ether of 4a,10a-dihydrofusarubin A was later isolated from F. solani, 8,9) and the 3-O-methyl ether of 4a,10a-dihydrofusarubin A together with the 3-O-ethyl ether was isolated from F. martii.…”