Quantitative Determination of Gallic Acid in Tannic Acid by Thin Layer Chromatography

Franiau, R.; Mussche, R.

doi:10.1002/j.2050-0416.1972.tb03479.x

Cited by 9 publications

(2 citation statements)

References 13 publications

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“…The nature of the main tannin contained in commercial extracts was determined after acid hydrolysis in a water bath. Proanthocyanidins were identified by appearance of an anthocyanidin after hydrolysis in butanol/HCl containing ferrous sulphate (Govindaragan and Mathew 1965), a red colour resulting from free anthocyanidins was recorded at 530 nm on a spectrophotometer (Fuleki and Francis 1968); gallotannins were determined under the conditions described by Franiau and Mussche (1972), gallic acid resulting from the hydrolysis of gallotannins in 1 M sulphuric acid was estimated by HPLC; ellagitannins were determined by methanol-HC1 degradation, and detection of ellagic acid by HPLC . The previously obtained values have been corrected to take account of the presence of free gallic acid and ellagic acid.…”

Section: Determination Of Tannin Naturementioning

confidence: 99%

Determination of the Composition of Commercial Tannin Extracts by Liquid Secondary Ion Mass Spectrometry (LSIMS)

Vivas¹,

Bourgeois

Vitry

et al. 1996

J. Sci. Food Agric.

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The composition of various commercial tannin extracts were determined by liquid secondary ion mass spectrometry (LSIMS). Spectra were obtained directly from tannin extracts without any pre‐separation. Eight different tannin powders were analysed: three gallotannins (Chinese, Turkish, tara), three ellagitannins (sweet chestnut, pendunculata oak, sessile oak), one mixed hydrolysable tannin (myrabolans) and one proanthocyanidin (grape seeds). This method enabled the main molecules in these powders to be identified.

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Section: Determination Of Tannin Naturementioning

confidence: 99%

Determination of the Composition of Commercial Tannin Extracts by Liquid Secondary Ion Mass Spectrometry (LSIMS)

Vivas¹,

Bourgeois

Vitry

et al. 1996

J. Sci. Food Agric.

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“…TA in solution can also be detected at 310 nm 34 . When bacteria were incubated in a 1000 µM TA solution, a decrease of absorption (about 50%) could be detected after 24 hours of incubation with Sgg (Fig.…”

Section: S Gallolyticus Ucn34 But Notmentioning

confidence: 99%

Streptococcus gallolyticus abrogates anti-carcinogenic properties of tannic acid on low-passage colorectal carcinomas

Oehmcke-Hecht

Mandl

Naatz

et al. 2020

Sci Rep

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the tannase-producing Gram-positive bacterial species Streptococcus gallolyticus subsp. gallolyticus (Sgg) is an opportunistic pathogen of the human gut and strongly associated with colorectal cancer (cRc). A unique feature of Sgg is its ability to degrade tannic acids (tA). tA constitute an important part of the human diet with known anti-tumorigenic properties. Here, we examined whether Sgg is able to protect tumor cells from the toxic effect of TA and thus drive tumorigenesis indirectly. Human CRC cell lines (n = 8) were treated with increasing concentrations of TA. We confirmed the cytotoxic activity of TA in a dose-dependent manner. In virtually all cell lines, viability decreased significantly (>60% inhibition). Moreover, pyrogallol, the degradation product of TA, had no effect on the tested cell lines. This suggests a specific effect of TA. Cytotoxicity was due to necrosis and induction of senescence in residual cells. finally, when tA was degraded by Sgg, the cytotoxic effect could be abolished. Tumor cells even responded with boosted cell proliferation, highlighting the impact of Sgg on cRc progression. We here provide another piece of evidence for the active interplay between Sgg and cancer preventive components. these data will help to move forward in designing concepts for therapeutic and eventually also prophylactic approaches to combat gastrointestinal malignancies.

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Digallic Acid

Verzele¹,

Delahaye²,

Dijck³

1983

Bulletin des Soc Chimique

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SUMMARYDigallic acid was prepared by partial acid hydrolysis of tannic acid and purified by Counter Current Distribution. In water and methanol solution digallic acid is present in two isomeric equilibrium forms which were named meta and para digallic acid. Meta digallic acid, the main component, is stable in ether and acetone solution. The above was proved by HPLC, GC-MS and NMR experiments on digallic acid and on its permethylation product.

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Quantitative Determination of Gallic Acid in Tannic Acid by Thin Layer Chromatography

Abstract: A method is described which allows the separation of compounds present in pyrogallol tannic acid by thin layer chromatography on previously purified silica gel.This method can be used to determine gallic acid in presence of gallotannin.

Cited by 9 publications

References 13 publications

Determination of the Composition of Commercial Tannin Extracts by Liquid Secondary Ion Mass Spectrometry (LSIMS)

Determination of the Composition of Commercial Tannin Extracts by Liquid Secondary Ion Mass Spectrometry (LSIMS)

Streptococcus gallolyticus abrogates anti-carcinogenic properties of tannic acid on low-passage colorectal carcinomas

Digallic Acid

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