Quantitative Determination of Flavonoids and Chlorogenic Acid in the Leaves of<i>Arbutus unedo</i>L. Using Thin Layer Chromatography

Maleš, Željan; Šarić, Darija; Bojić, Mirza

doi:10.1155/2013/385473

Cited by 29 publications

(19 citation statements)

References 12 publications

(9 reference statements)

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“…In the cinnamic group of phenolics, chlorogenic acid was the major phenolic acid. However, chlorogenic acid had previously been reported only in the leaves of the strawberry tree, but not in its fruit (Maleš et al, 2013).…”

Section: Resultsmentioning

confidence: 94%

Diversity in phenolic compounds, biochemical and pomological characteristics of Arbutus unedo fruits

Gündoğdu

Erċışlı

Canan

et al. 2018

Folia Horticulturae

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Strawberry trees (Arbutus unedoL.) are naturally grown in particular Black Sea and Mediterranean regions of Turkey with great diversity due to continuous seed propagation for centuries. The trees differ in terms of most of the horticultural characteristics. We investigated the phenolic compounds and the biochemical and pomological characteristics of the fruits of eight strawberry tree selections naturally grown in the western part of Turkey. Significant differences were found among the genotypes in terms of their phenolic compounds and their biochemical and pomological characteristics. Among soluble sugars, fructose (11.63 g 100 g−1) was the dominant sugar, followed by glucose (6.10 g 100 g−1) and sucrose (1.44 g 100 g−1) for all the genotypes. Positive correlation was found between fruit weight and soluble sugar content. Malic acid was the major organic acid (0.67-2.33 g 100 g−1), and the second major organic acid in strawberry tree fruits was citric acid (0.25-0.87 g 100 g−1). Vitamin C content was an average of 56.22 g 100 g−1for the eight genotypes. Among phenolic compounds, gallic acid was dominant (1.62-7.29 mg 100 g−1), followed by chlorogenic acid (1.23-3.14 mg 100 g−1), on an average basis.

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Section: Resultsmentioning

confidence: 94%

Diversity in phenolic compounds, biochemical and pomological characteristics of Arbutus unedo fruits

Gündoğdu

Erċışlı

Canan

et al. 2018

Folia Horticulturae

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“…The plate was put into the jar that contains mobile phase composed of toluene:ethyl acetate:methanol (5:3:2). The plate was put at the borderline and was observed by UV lamp at the wavelength 254 nm, and the Rf values were then measured (Males et al, 2013;Preethi et al, 2017;Rao & Ahmed, 2013).…”

Section: Methodsmentioning

confidence: 99%

Toxicity of Bioactive Compound from Endophytic Fungi Isolated from Red Ginger (Zingiber officinale var. rubrum) Utilizing Brine Shrimp Lethality Assay

Prasetyo

Hartini

Sidharta³

2019

bio

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Red ginger (Zingiber officinale var. rubrum) has been proven to show anticancer activity. Direct use bioactive compound from red ginger has many obstacles such as large amount of red ginger’s rhizome needed, limitation of planting area, and very long time of harvesting. Utilization of endophytic fungi from red ginger’s rhizome could be an alternative to the problems. The aims of this study were to determine bioactive compound produced by endophytic fungi and toxicity activity based on LC50. Endophytic fungi were isolated from red ginger and were identified macroscopically and microscopically. The bioactive compounds were extracted using ethanol 96%. Flavonoid test was done qualitatively, bioactive compounds were analyzed by Thin Layer Chromatography (TLC), and the toxicity test was done using Brine Shrimp Lethality Assay (BSLA). The present research found two endophytic fungi isolated from red ginger rhizome. Isolate 1 was similar to Mucor sp. and isolate 2 was similar to Trichoderma sp. Phytochemical test revealed bioactive compound extracted from the isolates were contained flavonoid. TLC analysis did not detect quercetin from the bioactive compound extracted from the isolates. LC50 values of the bioactive compound from the isolates were 2.300 and 1.747 µg/ml, respectively. The toxicological results suggest that both isolates produce non-toxic compound to Artemia salina.

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“…Arbutin was reported as being present in leaf extracts of A. unedo collected in Montenegro, along with hydroquinone derivatives [46]. Quercitrin, isoquercitrin, hyperoside, and chlorogenic acid are other phenolic compounds identified and quantified by [5,47] in extracts of leaves of A. unedo from Croatia. In this work, the authors also reported that the concentrations of these compounds changed over the year.…”

Section: Chemical Composition Of a Unedomentioning

confidence: 99%

Arbutus unedo L.: Chemical and Biological Properties

et al. 2014

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Arbutus unedo L. (strawberry tree) has a circum-Mediterranean distribution, being found in western, central and southern Europe, north-eastern Africa (excluding Egypt and Libya) and the Canary Islands and western Asia. Fruits of the strawberry tree are generally used for preparing alcoholic drinks (wines, liqueurs and brandies), jams, jellies and marmalades, and less frequently eaten as fresh fruit, despite their pleasing appearance. An overview of the chemical composition of different parts of the plant, strawberry tree honey and strawberry tree brandy will be presented. The biological properties of the different parts of A. unedo and strawberry tree honey will be also overviewed.

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Quantitative Determination of Flavonoids and Chlorogenic Acid in the Leaves ofArbutus unedoL. Using Thin Layer Chromatography

Cited by 29 publications

References 12 publications

Diversity in phenolic compounds, biochemical and pomological characteristics of Arbutus unedo fruits

Diversity in phenolic compounds, biochemical and pomological characteristics of Arbutus unedo fruits

Toxicity of Bioactive Compound from Endophytic Fungi Isolated from Red Ginger (Zingiber officinale var. rubrum) Utilizing Brine Shrimp Lethality Assay

Arbutus unedo L.: Chemical and Biological Properties

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