Quantitative Determination of drugs &amp; pharnaceuticalsby using iodine as analytical reagent: A spectrophotometric study

Kumar, T. Santhosh; Seethamma, M.; Venkateshwarlu, G.

doi:10.9790/5736-07520715

Cited by 7 publications

(4 citation statements)

References 38 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Iodine works as an electron acceptor and forms molecular complexes having a wide variety of compounds with electron-donating properties [48][49][50][51][52][53]. These studies demonstrated that iodine is a highly reactive σ-acceptor by virtue of its immediate reactions at ambient temperature.…”

Section: Strategy and Design Of The Studymentioning

confidence: 99%

Spectrophotometric Investigations of Charge Transfer Complexes of Tyrosine Kinase Inhibitors with Iodine as a σ-Electron Acceptor: Application to Development of Universal High-Throughput Microwell Assay for Their Determination in Pharmaceutical Formulations

Darwish

Ali

et al. 2023

Medicina

View full text Add to dashboard Cite

Background and Objective: Tyrosine kinase inhibitors (TKIs) are used for the treatment of different types of cancers. The current study describes, for the first time, the ultraviolet-visible spectrophotometric investigation of charge transfer complexes (CTCs) of seven TKIs, as electron donors, and iodine, as σ-electron. Materials and Methods: The formation of CTCs was promoted in dichloromethane, among the other solvents used in the investigation. The molar absorptivity values, association constants, and free energy changes of the CTCs were determined. Stoichiometric ratio of TKI: iodine as well as TKIs site(s) of interaction were addressed. Reaction was the basis for constructing a novel simple and accurate 96-microwell spectrophotometric assay (MW-SPA) with high-throughput property for the quantitative determination of TKIs in their pharmaceutical formulations. Results: Beer’s law, which relates CTC absorbances to TKI concentrations, was followed within the optimal range of 2 to 100 µg/well (r ranged from 0.9991 to 0.9998). Detection and quantification limits ranged from 0.91 to 3.60 and 2.76 to 10.92 g µmL−1, respectively. Relative standard deviations values for the intra- and inter-assay precisions of the proposed MW-SPA did not exceed 2.13 and 2.34%, respectively. Studies of recovery demonstrated MW-SPA accuracy, with results ranging from 98.9% to 102.4%. All TKIs, both in bulk form and in pharmaceutical formulations (tablets), were effectively determined using the suggested MW-SPA. Conclusions: The current MW-SPA involved a simple procedure and it was convenient as it could analyse all proposed TKIs utilizing a single assay system at once measuring wavelengths for all TKIs. In addition, the proposed MW-SPA has high throughput which enables the processing of a batch of huge samples’ number in very short reasonable time period. In conclusion, TKIs can be routinely analysed in their dosage forms in quality control laboratories, and the assay can be highly valuable and helpful in this regard.

show abstract

Section: Strategy and Design Of The Studymentioning

confidence: 99%

Spectrophotometric Investigations of Charge Transfer Complexes of Tyrosine Kinase Inhibitors with Iodine as a σ-Electron Acceptor: Application to Development of Universal High-Throughput Microwell Assay for Their Determination in Pharmaceutical Formulations

Darwish

Ali

et al. 2023

Medicina

View full text Add to dashboard Cite

show abstract

“…Moxifloxacin is chemically known as [1-Cyclopropyl-b-fluoro-1, 4-dihydro-8-methoxy-7-[(4aS, 7aS)-octahydro-6H-pyrrolo [3,[4][5][6] pyridine-6-yl]-4-oxo-3-quinoline carboxylic acid, is a fluoroquinolone broad-spectrum antibiotic agent and used in conjunctivitis. A few analytical techniques have been constructed for quantification of several drugs based on literature survey such as UV Spectrophotometry [33][34][35][36] RP-HPLC [37][38][39][40] , HPLC [41][42] , HPLC-MS [43][44] , LC-MS 45 , LC 46 , chemiluminescence 47 , Tanden mass spectrometry 48 methods for determination of Moxifloxacin have been reported.…”

Section: Gemifloxacin Chemically Is [{Rs}7-[{4z}-3-{aminomethyl}-mentioning

confidence: 99%

Untitled

2019

IJPSR

View full text Add to dashboard Cite

Simple, sensitive and selective methods are developed for the spectrophotometric determination of drugs, viz., Cetirizine hydrochloride, Duloxetine hydrochloride, Esmolol hydrochloride, Gemifloxacin, Moxifloxacin and Phenylephrine Hydrochloride based on their reactivity towards Chloramine-T (ChL-T). The method is based on the oxidation of drugs by ChL-T (Excess amount) in presence of acidic medium and estimating the amount of un-reacted ChL-T by Safranin-O (SO) dye at λmax 524 nm. The amount of ChL-T reacted is equal to the drug concentration in this method. The concentration of ChL-T is 10 µgmL-1 and Safranin-O Dye is 10 µgmL-1. The calibration curves obeyed Beer's law over the concentration range of 2.5-17.5 µgmL-1 (CET), 5-35 µgmL-1 (DUL), 12-84 µgmL-1 (ESM), 10-70 µgmL-1 (GEM) & 2.5-17.5 µgmL-1 (MOX). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. The method has been validated in terms of guidelines of International Conference on Harmonization. INTRODUCTION: Cetirizine dihydrochloride chemically is [2-[4-[(4-chlorophenyl) phenyl methyl]-1 piperazinyl] ethoxy] acetic acid di HCl. Cetirizine di HCl is belonged to the group of the second-generation h1 antagonist, inhibits allergic reaction mediated by histamine.

show abstract

“…The methods which were reported in the literature for the determination of RAM includes Spectrophotometry [9][10][11][12], derivative Spectrophotometry [13] Extractive Spectrophotometry [14], Chromatography [15], High Performance Liquid Chromatography(HPLC) [16], conductometry [17]. A few techniques, including RP-HPLC [18][19][20][21][22], UV-Spectrophotometry [23][24][25][26][27][28], LC-Mass spectrometry [LC-MS] [ [29][30],…”

Section: Ramipril (Figure1b) Is Known As -1-[2-1-(ethoxycarbonyl)-3-phenylpropyl] Amino]mentioning

confidence: 99%

Simple Spectrophotometric Method for Estimation of Drugs and Pharmaceuticals by using Chloramine-T and Safranin-O Dye Couple

2018

AJBPR

View full text Add to dashboard Cite

Simple, sensitive and selective methods are developed for the indirect and oxidative spectrophotometric determination of drugs, viz., Phenylephrine Hydrochloride, Ramipril Hydrochloride, Sumatriptan Succinate, Tramadol hydrochloride and Trimetazidine is based on their reactivity towards Chloramine-T (ChL-T). The method is based on the oxidation of drugs by ChL-T (Excess quantity) and estimating the amount of un-reacted ChL-T by Safranin-O dye at λmax 524 nm. The concentration reacted Chloramine-T is equal to the Drug concentration in this method. The concentration of Safranin-O and Chloramine-T is 10 µg mL-1. The calibration curves obeyed Beer's law over the concentration range of 10-70 µg mL-1 (PHP), 5-35 µg mL-1 (RAM), 5-35 µg mL-1 (SUM), 10-70 µg mL-1 (TRA) & 12-84 µg mL-1 (TMT). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. The method has been validated in terms of guidelines of International Conference on Harmonization.

show abstract

Quantitative Determination of drugs & pharnaceuticalsby using iodine as analytical reagent: A spectrophotometric study

Cited by 7 publications

References 38 publications

Spectrophotometric Investigations of Charge Transfer Complexes of Tyrosine Kinase Inhibitors with Iodine as a σ-Electron Acceptor: Application to Development of Universal High-Throughput Microwell Assay for Their Determination in Pharmaceutical Formulations

Spectrophotometric Investigations of Charge Transfer Complexes of Tyrosine Kinase Inhibitors with Iodine as a σ-Electron Acceptor: Application to Development of Universal High-Throughput Microwell Assay for Their Determination in Pharmaceutical Formulations

Untitled

Simple Spectrophotometric Method for Estimation of Drugs and Pharmaceuticals by using Chloramine-T and Safranin-O Dye Couple

Contact Info

Product

Resources

About