Simple Spectrophotometric Method for Estimation of Drugs and Pharmaceuticals by using Chloramine-T and Safranin-O Dye Couple

Abstract

Simple, sensitive and selective methods are developed for the indirect and oxidative spectrophotometric determination of drugs, viz., Phenylephrine Hydrochloride, Ramipril Hydrochloride, Sumatriptan Succinate, Tramadol hydrochloride and Trimetazidine is based on their reactivity towards Chloramine-T (ChL-T). The method is based on the oxidation of drugs by ChL-T (Excess quantity) and estimating the amount of un-reacted ChL-T by Safranin-O dye at λmax 524 nm. The concentration reacted Chloramine-T is equal to the Drug concentration in this method. The concentration of Safranin-O and Chloramine-T is 10 µg mL-1. The calibration curves obeyed Beer's law over the concentration range of 10-70 µg mL-1 (PHP), 5-35 µg mL-1 (RAM), 5-35 µg mL-1 (SUM), 10-70 µg mL-1 (TRA) & 12-84 µg mL-1 (TMT). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. The method has been validated in terms of guidelines of International Conference on Harmonization.