Quantitative determination of dopamine in single rat pheochromocytoma cells by microchip electrophoresis with only one high‐voltage power supply

Abstract

We developed a method for the direct identification of dopamine in single cultured rat pheochromocytoma cells by capillary electrophoresis using an end-channel carbon fiber nanoelectrode amperometric detector. The operation mode was designed to achieve single-cell injection and lysis in microfluidic chip electrophoresis with only one high-voltage power supply. The separation and detection conditions were optimized. Four catecholamines were baseline-separated and determined with this system, and the cell density and liquid height of the reservoirs were accommodated for single cell loading, docking and analysis. The microchip capillary electrophoresis system was successfully applied to determine dopamine in single cultured rat pheochromocytoma cells.

Facile Fluorescence Dopamine Detection Strategy Based on Acid Phosphatase (ACP) Enzymatic Oxidation Dopamine to Polydopamine

“…To date, a variety of analytical methods, such as electrochemistry [9][10] , capillary electrophoresis [11][12] , chemiluminescence 13) , and high-performance liquid chromatography (HPLC) [14][15] , have been developed for DA detection. Although the abovementioned strategies offer efficient and sensitive DA sensing technologies, they face certain constraints of complex procedure, consuming time, and special equipment, thus further development is needed to achieve the practical requirements of convenient and accurate determination for DA.…”

Efficient strategy for the selective determination of dopamine in human urine by molecularly imprinted solid‐phase extraction

“…Recently, several original papers showed the great diversity of novel methodologies of DA determination . Nevertheless, their usefulness is hampered by the high costs of analysis and the physicochemical properties of DA, an aliphatic amine which can be easily protonated and in consequence can be soluble in polar solvents .…”